Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KFU83_RS03835 Genome accession   NZ_CP073939
Coordinates   770018..770998 (+) Length   326 a.a.
NCBI ID   WP_072034998.1    Uniprot ID   -
Organism   Escherichia coli strain MB53     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 765018..775998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU83_RS03805 (KFU83_03750) yggM 765446..766453 (+) 1008 WP_040062534.1 DUF1202 family protein -
  KFU83_RS03810 (KFU83_03755) hemW 766695..767831 (-) 1137 WP_000239939.1 radical SAM family heme chaperone HemW -
  KFU83_RS03815 (KFU83_03760) rdgB 767824..768417 (-) 594 WP_001174752.1 XTP/dITP diphosphatase -
  KFU83_RS03820 (KFU83_03765) yggU 768425..768715 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  KFU83_RS03825 (KFU83_03770) yggT 768712..769278 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  KFU83_RS03830 (KFU83_03775) yggS 769296..770000 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  KFU83_RS03835 (KFU83_03780) pilT 770018..770998 (+) 981 WP_072034998.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KFU83_RS03840 (KFU83_03785) ruvX 771174..771590 (-) 417 WP_077137658.1 Holliday junction resolvase RuvX -
  KFU83_RS03845 (KFU83_03790) yqgE 771590..772153 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  KFU83_RS03850 (KFU83_03795) gshB 772262..773212 (-) 951 WP_040062533.1 glutathione synthase -
  KFU83_RS03855 (KFU83_03800) rsmE 773225..773956 (-) 732 WP_257188253.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  KFU83_RS03860 (KFU83_03805) endA 774036..774743 (-) 708 WP_000286500.1 deoxyribonuclease I -
  KFU83_RS03865 (KFU83_03810) yggI 774838..775335 (-) 498 WP_257188254.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35939.06 Da        Isoelectric Point: 5.9554

>NTDB_id=488423 KFU83_RS03835 WP_072034998.1 770018..770998(+) (pilT) [Escherichia coli strain MB53]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHSPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=488423 KFU83_RS03835 WP_072034998.1 770018..770998(+) (pilT) [Escherichia coli strain MB53]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACAGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGTTATCTCAATCAACATGCTGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAGGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCGGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTACTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(61-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilB Legionella pneumophila strain ERS1305867

31.152

100

0.365