Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KEW59_RS15340 Genome accession   NZ_CP073621
Coordinates   3230744..3231724 (+) Length   326 a.a.
NCBI ID   WP_001596138.1    Uniprot ID   -
Organism   Escherichia coli strain CE2050     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3225744..3236724
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KEW59_RS15310 (KEW59_15180) - 3226122..3227108 (-) 987 WP_000784010.1 TRAP transporter substrate-binding protein -
  KEW59_RS15315 (KEW59_15185) hemW 3227421..3228557 (-) 1137 WP_000239947.1 radical SAM family heme chaperone HemW -
  KEW59_RS15320 (KEW59_15190) rdgB 3228550..3229143 (-) 594 WP_001174736.1 XTP/dITP diphosphatase -
  KEW59_RS15325 (KEW59_15195) yggU 3229151..3229441 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  KEW59_RS15330 (KEW59_15200) yggT 3229438..3230004 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  KEW59_RS15335 (KEW59_15205) yggS 3230022..3230726 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  KEW59_RS15340 (KEW59_15210) pilT 3230744..3231724 (+) 981 WP_001596138.1 type IV pilus twitching motility protein PilT Machinery gene
  KEW59_RS15345 (KEW59_15215) ruvX 3231898..3232314 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  KEW59_RS15350 (KEW59_15220) yqgE 3232314..3232877 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  KEW59_RS15355 (KEW59_15225) gshB 3232986..3233936 (-) 951 WP_000593260.1 glutathione synthase -
  KEW59_RS15360 (KEW59_15230) rsmE 3233949..3234680 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  KEW59_RS15365 (KEW59_15235) endA 3234760..3235467 (-) 708 WP_001305312.1 deoxyribonuclease I -
  KEW59_RS15370 (KEW59_15240) yggI 3235562..3236059 (-) 498 WP_032160294.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36057.30 Da        Isoelectric Point: 5.9983

>NTDB_id=487449 KEW59_RS15340 WP_001596138.1 3230744..3231724(+) (pilT) [Escherichia coli strain CE2050]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFEAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VKEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=487449 KEW59_RS15340 WP_001596138.1 3230744..3231724(+) (pilT) [Escherichia coli strain CE2050]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGAGGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAACAGCGGGAGATTGGTTTGCACTGTATGACTTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGTTGTTTGAATTGCTGATTAACACACCTGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.566

100

0.457
  pilT Legionella pneumophila strain Lp02

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

97.546

0.414
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362