Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IG283_RS19505 Genome accession   NZ_CP062220
Coordinates   3985954..3986934 (-) Length   326 a.a.
NCBI ID   WP_079977203.1    Uniprot ID   A0A5I4KPX4
Organism   Salmonella enterica subsp. enterica serovar Kottbus strain Kharkiv     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3980954..3991934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IG283_RS19475 (IG283_19475) endA 3981412..3982119 (+) 708 WP_000286122.1 deoxyribonuclease I -
  IG283_RS19480 (IG283_19480) rsmE 3982196..3982927 (+) 732 WP_001222489.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  IG283_RS19485 (IG283_19485) gshB 3982947..3983894 (+) 948 WP_000593248.1 glutathione synthase -
  IG283_RS19490 (IG283_19490) - 3984110..3984673 (+) 564 WP_001053173.1 YqgE/AlgH family protein -
  IG283_RS19495 (IG283_19495) ruvX 3984673..3985089 (+) 417 WP_001014571.1 Holliday junction resolvase RuvX -
  IG283_RS19500 (IG283_19500) - 3985136..3985822 (-) 687 WP_000098324.1 IclR family transcriptional regulator -
  IG283_RS19505 (IG283_19505) pilT 3985954..3986934 (-) 981 WP_079977203.1 type IV pilus twitching motility protein PilT Machinery gene
  IG283_RS19510 (IG283_19510) - 3986952..3987656 (+) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  IG283_RS19515 (IG283_19515) - 3987675..3988241 (+) 567 WP_001094848.1 YggT family protein -
  IG283_RS19520 (IG283_19520) yggU 3988238..3988528 (+) 291 WP_001277203.1 DUF167 family protein YggU -
  IG283_RS19525 (IG283_19525) - 3988536..3989129 (+) 594 WP_001174769.1 XTP/dITP diphosphatase -
  IG283_RS19530 (IG283_19530) hemW 3989122..3990258 (+) 1137 WP_001096532.1 radical SAM family heme chaperone HemW -
  IG283_RS19535 (IG283_19535) - 3990350..3991357 (-) 1008 WP_000252197.1 DUF1202 domain-containing protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36153.33 Da        Isoelectric Point: 7.5116

>NTDB_id=486963 IG283_RS19505 WP_079977203.1 3985954..3986934(-) (pilT) [Salmonella enterica subsp. enterica serovar Kottbus strain Kharkiv]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMENAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLAIRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTAATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QVQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=486963 IG283_RS19505 WP_079977203.1 3985954..3986934(-) (pilT) [Salmonella enterica subsp. enterica serovar Kottbus strain Kharkiv]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGAACGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ATGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGATACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAAT
CCACTACTCTGGCGGCGATGGTGGGATATCTTAATCAACATGCCGATAAACATATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGTAGTCTGCGCGCTGTACTATCGCAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGGCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGTGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5I4KPX4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.318

100

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.318

100

0.485
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baumannii strain A118

45.26

100

0.454
  pilT Acinetobacter baumannii D1279779

45.26

100

0.454
  pilT Acinetobacter nosocomialis M2

46.082

97.853

0.451
  pilT Acinetobacter baylyi ADP1

44.954

100

0.451
  pilT Pseudomonas stutzeri DSM 10701

45.886

96.933

0.445
  pilT Pseudomonas aeruginosa PAK

45.886

96.933

0.445
  pilT Legionella pneumophila strain ERS1305867

44.969

97.546

0.439
  pilT Legionella pneumophila strain Lp02

44.969

97.546

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.509

97.546

0.405
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Pseudomonas stutzeri DSM 10701

36.254

100

0.368
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362