Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KCG54_RS11265 Genome accession   NZ_CP073115
Coordinates   2322351..2323397 (+) Length   348 a.a.
NCBI ID   WP_070606632.1    Uniprot ID   -
Organism   Neisseria subflava strain TT0077     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2317351..2328397
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCG54_RS11245 (KCG54_11255) dksA 2319454..2319870 (-) 417 WP_004519243.1 RNA polymerase-binding protein DksA -
  KCG54_RS11250 (KCG54_11260) - 2320104..2320661 (-) 558 WP_254324203.1 YggT family protein -
  KCG54_RS11255 (KCG54_11265) proC 2320664..2321461 (-) 798 WP_254324204.1 pyrroline-5-carboxylate reductase -
  KCG54_RS11260 (KCG54_11270) - 2321538..2322233 (-) 696 WP_254324205.1 YggS family pyridoxal phosphate-dependent enzyme -
  KCG54_RS11265 (KCG54_11275) pilT 2322351..2323397 (+) 1047 WP_070606632.1 type IV pilus twitching motility protein PilT Machinery gene
  KCG54_RS11270 (KCG54_11280) pilU 2323484..2324713 (+) 1230 WP_254324206.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KCG54_RS11275 (KCG54_11285) yccS 2324879..2327014 (+) 2136 WP_254324207.1 YccS family putative transporter -
  KCG54_RS11280 (KCG54_11290) - 2327178..2328356 (-) 1179 WP_004519232.1 phosphoglycerate kinase -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38460.07 Da        Isoelectric Point: 7.1311

>NTDB_id=486889 KCG54_RS11265 WP_070606632.1 2322351..2323397(+) (pilT) [Neisseria subflava strain TT0077]
MQITDLLAFGVKNKASDLHLSSGISPMIRVHGDIRRINLPEMSSEEVGTMITSVMNDHQRKLYQQDFEVDFSFELPNVAR
FRVNAFMTERGPAAVFRTIPSTVLTLEELRAPRIFQKIAENPRGLVLVTGPTGSGKSTTLAAMVNYINETQPAHILTIED
PIEFVHQSKKALINQRELHQHTHSFTNALRSALREDPDVILIGEMRDPETISLALTAAETGHLVFGTLHTTGAAKTIDRI
VDVFPAGEKEIVRSMLSESLRAIISQTLLKTRDGNGRVAAHEILISTPAVRNLIRENKIAQINAALQTGQAHGMQTLDQA
LQTLVRQGTISPELARSKAQNIENLSIF

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=486889 KCG54_RS11265 WP_070606632.1 2322351..2323397(+) (pilT) [Neisseria subflava strain TT0077]
ATGCAGATTACCGACTTACTCGCGTTCGGCGTGAAAAACAAAGCCTCCGACCTCCATTTGAGTTCGGGCATTTCCCCTAT
GATACGCGTACACGGCGACATCCGCCGCATCAACCTGCCCGAGATGAGCTCGGAAGAAGTCGGCACTATGATTACCTCGG
TCATGAACGACCACCAGCGTAAGCTCTATCAACAGGATTTTGAAGTCGATTTCTCATTTGAGCTGCCTAATGTTGCCCGT
TTCCGCGTCAACGCCTTTATGACCGAACGCGGCCCAGCCGCCGTATTCCGTACCATTCCGAGCACCGTCCTAACGCTGGA
AGAATTGCGTGCGCCGCGCATTTTCCAAAAAATTGCCGAAAATCCGCGCGGTTTGGTCTTGGTAACCGGCCCGACCGGTT
CGGGTAAATCCACCACGCTGGCGGCGATGGTCAACTACATCAACGAAACCCAACCAGCCCACATTCTGACCATCGAAGAC
CCGATTGAGTTCGTCCACCAAAGCAAAAAGGCGCTCATCAACCAACGCGAGCTGCATCAGCACACCCACAGCTTTACCAA
CGCCCTGCGTTCCGCTCTGCGCGAAGACCCAGACGTGATTTTGATCGGTGAGATGCGCGACCCTGAAACCATCTCCCTCG
CCCTGACCGCCGCCGAAACCGGCCACTTGGTTTTCGGCACGCTGCACACAACCGGCGCCGCTAAAACCATCGACCGTATT
GTCGACGTATTCCCAGCTGGAGAAAAAGAAATCGTGCGCTCCATGTTGTCCGAATCCTTGCGCGCCATCATTTCCCAAAC
CCTGCTGAAAACCCGCGACGGCAACGGCCGCGTCGCCGCACACGAAATCCTGATTTCCACACCGGCCGTACGCAACCTCA
TCCGCGAGAACAAAATCGCCCAAATCAACGCCGCCCTCCAAACCGGTCAGGCCCACGGTATGCAAACCCTCGATCAAGCC
CTGCAAACCCTTGTCCGCCAAGGCACCATCAGCCCCGAGCTGGCACGCAGCAAGGCGCAAAACATCGAAAATCTCTCTAT
TTTCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

86.455

99.713

0.862
  pilT Neisseria gonorrhoeae MS11

86.455

99.713

0.862
  pilT Acinetobacter nosocomialis M2

68.696

99.138

0.681
  pilT Acinetobacter baumannii D1279779

68.696

99.138

0.681
  pilT Acinetobacter baumannii strain A118

68.696

99.138

0.681
  pilT Pseudomonas stutzeri DSM 10701

68.116

99.138

0.675
  pilT Pseudomonas aeruginosa PAK

67.826

99.138

0.672
  pilT Acinetobacter baylyi ADP1

67.536

99.138

0.67
  pilT Legionella pneumophila strain Lp02

65.396

97.989

0.641
  pilT Legionella pneumophila strain ERS1305867

65.396

97.989

0.641
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65

97.701

0.635
  pilT Vibrio cholerae strain A1552

65

97.701

0.635
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.662

95.115

0.491
  pilU Pseudomonas stutzeri DSM 10701

41.092

100

0.411
  pilU Vibrio cholerae strain A1552

39.655

100

0.397
  pilU Acinetobacter baylyi ADP1

39.08

100

0.391