Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   IGX38_RS05025 Genome accession   NZ_CP062162
Coordinates   1053456..1053863 (-) Length   135 a.a.
NCBI ID   WP_419586397.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain 2020WEIHUA_G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1048456..1058863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IGX38_RS05005 (IGX38_04990) cysN 1048789..1050687 (+) 1899 WP_061374059.1 sulfate adenylyltransferase subunit CysN -
  IGX38_RS05010 (IGX38_04995) tfpZ 1050810..1051547 (-) 738 WP_236450819.1 TfpX/TfpZ family type IV pilin accessory protein -
  IGX38_RS05015 (IGX38_05000) - 1051556..1052947 (-) 1392 WP_242100619.1 O-antigen ligase family protein -
  IGX38_RS05020 (IGX38_05005) pilA/pilAII 1052972..1053397 (-) 426 WP_061374061.1 pilin Regulator
  IGX38_RS05025 (IGX38_05010) pilA/pilAI 1053456..1053863 (-) 408 WP_419586397.1 pilin Machinery gene
  IGX38_RS05030 (IGX38_05015) pilB 1054195..1055898 (+) 1704 WP_106442874.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IGX38_RS05035 (IGX38_05020) pilC 1055901..1057118 (+) 1218 WP_242100621.1 type II secretion system F family protein Machinery gene
  IGX38_RS05040 (IGX38_05025) pilD 1057123..1057989 (+) 867 WP_242100623.1 prepilin peptidase Machinery gene
  IGX38_RS05045 (IGX38_05030) coaE 1058120..1058728 (+) 609 WP_111481658.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 135 a.a.        Molecular weight: 14064.01 Da        Isoelectric Point: 4.8277

>NTDB_id=486612 IGX38_RS05025 WP_419586397.1 1053456..1053863(-) (pilA/pilAI) [Stutzerimonas stutzeri strain 2020WEIHUA_G]
MQKGFTLIELMIVVAIIGILAAIALPAYQDYTVRSNAAAALAEITPGKIGFEQAINEGKTPSLDPTEEGYIGITDSTSYC
GVTINPAANGNIECTAKGGNAGKFDGKKITLNRTAEGAWSCASDLESKYKPGKCS

Nucleotide


Download         Length: 408 bp        

>NTDB_id=486612 IGX38_RS05025 WP_419586397.1 1053456..1053863(-) (pilA/pilAI) [Stutzerimonas stutzeri strain 2020WEIHUA_G]
ATGCAGAAAGGCTTTACCCTTATCGAACTGATGATCGTGGTTGCGATCATCGGCATTCTGGCCGCCATTGCGCTGCCGGC
TTATCAGGACTATACCGTACGCTCCAACGCTGCCGCTGCCTTGGCAGAAATCACCCCCGGCAAAATCGGTTTTGAGCAGG
CGATCAACGAAGGAAAAACTCCGTCGCTCGACCCAACCGAGGAAGGCTACATTGGTATAACCGACTCTACTTCTTACTGT
GGAGTGACTATTAATCCTGCTGCAAATGGCAATATCGAGTGTACTGCCAAAGGCGGCAATGCCGGCAAGTTTGATGGCAA
AAAAATCACGCTTAATCGCACCGCCGAGGGAGCGTGGAGCTGCGCTAGCGACTTGGAATCCAAGTACAAGCCAGGCAAGT
GTTCCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(33-134)

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

88.148

100

0.881
  pilA/pilAII Pseudomonas stutzeri DSM 10701

65.909

97.778

0.644
  pilA Pseudomonas aeruginosa PAK

45.89

100

0.496
  pilA/pilA1 Eikenella corrodens VA1

40.385

100

0.467
  pilA Acinetobacter baumannii strain A118

45

100

0.467
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.727

100

0.43
  pilA Vibrio cholerae strain A1552

41.727

100

0.43
  pilA Vibrio cholerae C6706

41.727

100

0.43
  comP Acinetobacter baylyi ADP1

40

100

0.43
  pilA2 Legionella pneumophila str. Paris

40.741

100

0.407
  pilA2 Legionella pneumophila strain ERS1305867

40.741

100

0.407
  pilA Vibrio parahaemolyticus RIMD 2210633

36.296

100

0.363