Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IGX38_RS05030 Genome accession   NZ_CP062162
Coordinates   1054195..1055898 (+) Length   567 a.a.
NCBI ID   WP_106442874.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain 2020WEIHUA_G     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1049195..1060898
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IGX38_RS05010 (IGX38_04995) tfpZ 1050810..1051547 (-) 738 WP_236450819.1 TfpX/TfpZ family type IV pilin accessory protein -
  IGX38_RS05015 (IGX38_05000) - 1051556..1052947 (-) 1392 WP_242100619.1 O-antigen ligase family protein -
  IGX38_RS05020 (IGX38_05005) pilA/pilAII 1052972..1053397 (-) 426 WP_061374061.1 pilin Regulator
  IGX38_RS05025 (IGX38_05010) pilA/pilAI 1053456..1053863 (-) 408 WP_419586397.1 pilin Machinery gene
  IGX38_RS05030 (IGX38_05015) pilB 1054195..1055898 (+) 1704 WP_106442874.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IGX38_RS05035 (IGX38_05020) pilC 1055901..1057118 (+) 1218 WP_242100621.1 type II secretion system F family protein Machinery gene
  IGX38_RS05040 (IGX38_05025) pilD 1057123..1057989 (+) 867 WP_242100623.1 prepilin peptidase Machinery gene
  IGX38_RS05045 (IGX38_05030) coaE 1058120..1058728 (+) 609 WP_111481658.1 dephospho-CoA kinase -
  IGX38_RS05050 (IGX38_05035) yacG 1058725..1058922 (+) 198 WP_095460669.1 DNA gyrase inhibitor YacG -
  IGX38_RS05055 (IGX38_05040) - 1058935..1059615 (-) 681 WP_013981946.1 energy-coupling factor ABC transporter permease -
  IGX38_RS05060 (IGX38_05045) - 1059673..1060713 (-) 1041 WP_014595961.1 NADP(H)-dependent aldo-keto reductase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62085.04 Da        Isoelectric Point: 5.4725

>NTDB_id=486613 IGX38_RS05030 WP_106442874.1 1054195..1055898(+) (pilB) [Stutzerimonas stutzeri strain 2020WEIHUA_G]
MSENISLSGLARQMVLAGVLDERTAQQAQVQALRNKTSFVTYVVQNKLAKGRAVAEVASEQFGVALLDLAALDKEVPPKD
VVSEKLARQHRLLPLAKRGNKLYVAVSDPANHQAISDIQFSTGLTTEAILVEDDKLGDAIDKFFDNGSTGLEDLGDVDLD
GVDVETVSDDIKGDDGGAEADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAYRVRLRTDGILHEVARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKSKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEDQKELYMNALRQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCNSCKKEVDVPRDVLLREGFP
EQKLGTFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPSLQRIIMEDGNSIDISTQMRKDGFNDLRTSALLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=486613 IGX38_RS05030 WP_106442874.1 1054195..1055898(+) (pilB) [Stutzerimonas stutzeri strain 2020WEIHUA_G]
ATGAGCGAAAATATTTCTCTGTCCGGGTTGGCTCGGCAGATGGTGTTGGCTGGTGTACTGGATGAGCGGACAGCACAGCA
GGCGCAGGTTCAGGCGCTGCGAAACAAAACATCCTTCGTTACCTACGTAGTCCAGAATAAATTGGCAAAGGGGCGTGCCG
TCGCTGAAGTTGCCTCGGAGCAGTTCGGCGTTGCGCTCTTGGATCTGGCGGCTCTGGACAAGGAGGTGCCGCCCAAGGAT
GTGGTCAGCGAGAAGCTGGCTCGCCAGCATCGCTTGCTCCCGCTGGCTAAGCGCGGCAATAAGCTTTACGTGGCCGTTTC
AGATCCGGCGAACCATCAGGCGATTTCCGATATTCAGTTCAGTACTGGGTTAACCACCGAGGCCATCCTCGTCGAGGACG
ACAAGCTCGGGGACGCCATCGATAAGTTTTTCGATAATGGTTCAACCGGTCTGGAGGATCTGGGCGACGTGGATCTCGAC
GGAGTGGATGTCGAGACCGTTTCGGATGACATAAAAGGAGACGATGGCGGTGCGGAGGCTGACGATGCGCCGGTCGTGCG
CTTCGTCAACAAGATGCTGCTCGACGCCATTCGTGGGGGCTCATCAGACCTCCATTTCGAGCCCTATGAAAAGGCCTATC
GAGTTCGGTTGCGTACTGACGGAATTCTGCATGAGGTGGCGCGCCCGCCGATTCAACTCGCGCCTCGAATTTCGGCGCGT
CTAAAGGTCATGGCAGGCCTGGATATCTCCGAGCGGCGCAAGCCGCAGGATGGCCGCATCAAGATGAAACTCTCCAAGAG
CAAGGCCATCGATTTCCGGGTCAACACGCTACCGACCCTGTGGGGCGAAAAGATCGTGATGCGGATTCTTGATCCCTCAA
GTGCTCAAATGGGCATCGATGCGCTGGGGTATGAGGAGGATCAAAAAGAGCTGTATATGAATGCTCTGCGGCAACCGCAG
GGTATGATTCTCGTGACTGGTCCGACGGGCTCCGGTAAGACGGTATCCCTCTACACCGGGTTGAACATCCTCAACACCGT
CGATGTGAATATTTCGACGGCGGAGGATCCGGTAGAGATCAACCTCGAGGGTATCAACCAGGTCAACGTCAATCCTAAAC
AGGGCATGGACTTTTCTCAGGCCCTGCGTGCCTTTCTACGTCAGGACCCGGACATCATCATGGTAGGGGAGATCCGCGAT
CTGGAGACCGCCGAGATTGCGATCAAGGCTGCGCAGACGGGCCATATGGTGATGTCGACACTTCATACCAATAGCGCTGC
GGAAACCCTGACGCGTCTGCGCAACATGGGGGTGCCTGCGTTCAACATCGCGACCTCCGTGAACCTGATCATTGCTCAGC
GTCTGGCACGAAAGCTCTGCAATAGCTGCAAGAAAGAGGTTGATGTCCCCCGTGACGTTTTGCTGCGCGAAGGGTTCCCT
GAGCAGAAGCTTGGAACCTTCAAGATTTATGGTCCGGTAGGTTGCGACAACTGCAAGGGCGGCTACAAGGGGCGCGTTGG
TATTTATGAAGTGGTTAAAAACACGCCATCGTTGCAGCGGATTATCATGGAAGACGGAAACTCCATCGATATTTCAACCC
AGATGCGCAAAGACGGCTTCAACGACCTGCGGACCTCGGCTTTGCTCAAGGCCATGCAGGGCGTGACCAGCCTCGAAGAA
GTCAATCGGGTCACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.638

99.471

0.603
  pilB Acinetobacter baylyi ADP1

59.574

99.471

0.593
  pilB Legionella pneumophila strain ERS1305867

55.769

100

0.563
  pilB Vibrio cholerae strain A1552

51.287

95.944

0.492
  pilB Vibrio campbellii strain DS40M4

48.246

100

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

51.923

91.711

0.476
  pilF Neisseria gonorrhoeae MS11

51.731

91.711

0.474
  pilB/pilB1 Synechocystis sp. PCC 6803

35.91

100

0.393