Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IF654_RS01875 Genome accession   NZ_CP062158
Coordinates   344108..345103 (+) Length   331 a.a.
NCBI ID   WP_194862808.1    Uniprot ID   -
Organism   Pseudomonas lundensis strain 2T.2.5.2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 339108..350103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF654_RS01840 (IF654_09080) - 339239..339676 (-) 438 WP_169880112.1 DUF4426 domain-containing protein -
  IF654_RS01845 (IF654_09075) metW 339702..340322 (-) 621 WP_047279459.1 methionine biosynthesis protein MetW -
  IF654_RS01850 (IF654_09070) metX 340330..341469 (-) 1140 WP_047278370.1 homoserine O-succinyltransferase MetX -
  IF654_RS01855 (IF654_09065) - 341618..341914 (-) 297 WP_047279458.1 DUF167 domain-containing protein -
  IF654_RS01860 (IF654_09060) - 341914..342501 (-) 588 WP_194862809.1 YggT family protein -
  IF654_RS01865 (IF654_09055) proC 342513..343331 (-) 819 WP_094988773.1 pyrroline-5-carboxylate reductase -
  IF654_RS01870 (IF654_09050) - 343364..344050 (-) 687 WP_047281473.1 YggS family pyridoxal phosphate-dependent enzyme -
  IF654_RS01875 (IF654_09045) pilT 344108..345103 (+) 996 WP_194862808.1 type IV pilus twitching motility protein PilT Machinery gene
  IF654_RS01880 (IF654_09040) - 345145..345792 (-) 648 WP_047279454.1 C40 family peptidase -
  IF654_RS01885 (IF654_09035) - 346045..346461 (+) 417 WP_047278349.1 NINE protein -
  IF654_RS01890 (IF654_09030) - 346531..347802 (-) 1272 WP_194862807.1 dihydroorotase -
  IF654_RS01895 (IF654_09025) - 347799..348803 (-) 1005 WP_047278345.1 aspartate carbamoyltransferase catalytic subunit -
  IF654_RS01900 (IF654_09020) pyrR 348816..349334 (-) 519 WP_047278343.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  IF654_RS01905 (IF654_09015) ruvX 349417..349857 (-) 441 WP_047278341.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 331 a.a.        Molecular weight: 35983.55 Da        Isoelectric Point: 6.6338

>NTDB_id=486551 IF654_RS01875 WP_194862808.1 344108..345103(+) (pilT) [Pseudomonas lundensis strain 2T.2.5.2]
MDIADLLAVCVARGASDMHLSAGVEPLLRIDGDLQRCELPSVSAQCVLELIHSMMTASQREAFETTLEADFSYTVPELGR
FRVNAFRQQHGPAAVLRRIGATVPSLASLGLEQVLSPLFNVPHGLILITGATGSGKTTTLAALIDHLNSTRQQHILTIED
PIEFVHTSRRCLINQREVHRDTHGFANALRSALREDPDVIVLGEMRDLDSIRLALTAAETGHLVFATLHTPSAAKTIDRI
VDVFAAEEKAMVRAMLSESLQAVVSQTLLKKKDGGRVAAHEIMLGTPAIRNLIRENKVAQMASVIQTGAAIGMQTLDMSV
KRLQERGIIDL

Nucleotide


Download         Length: 996 bp        

>NTDB_id=486551 IF654_RS01875 WP_194862808.1 344108..345103(+) (pilT) [Pseudomonas lundensis strain 2T.2.5.2]
ATGGATATCGCCGACCTGTTGGCTGTATGCGTGGCGCGCGGCGCATCGGACATGCACCTGTCTGCGGGCGTGGAGCCGCT
GCTGCGCATTGATGGCGACCTGCAGCGGTGCGAGCTGCCGAGCGTAAGTGCGCAGTGTGTACTCGAACTTATCCACAGCA
TGATGACCGCGTCGCAGCGCGAGGCGTTTGAAACAACCCTCGAAGCTGATTTTTCGTACACCGTGCCGGAGCTGGGGCGG
TTTCGGGTGAATGCCTTCAGGCAACAGCATGGCCCCGCAGCGGTGTTGCGCCGTATTGGCGCCACGGTGCCGAGCCTGGC
ATCACTGGGTTTGGAGCAAGTGCTGAGCCCGCTCTTTAACGTGCCTCATGGTCTGATCCTGATCACCGGCGCCACGGGTT
CAGGCAAAACCACCACGCTGGCCGCCCTCATCGATCACCTCAACAGCACGCGTCAGCAACACATTCTGACCATTGAAGAC
CCGATTGAATTCGTTCACACCTCGCGACGCTGCCTGATCAATCAGCGCGAAGTCCACCGCGACACCCACGGTTTCGCCAA
CGCATTGCGCTCAGCGTTGCGTGAAGACCCGGACGTGATTGTGCTTGGTGAAATGCGCGACCTCGACAGTATCCGGTTGG
CCCTGACCGCCGCAGAAACCGGGCACCTGGTGTTTGCCACGCTGCACACGCCCAGTGCGGCCAAGACCATTGACCGCATC
GTCGATGTGTTTGCGGCCGAAGAGAAAGCCATGGTGCGGGCGATGCTGTCCGAATCACTGCAGGCAGTGGTGTCTCAGAC
GTTGCTCAAGAAAAAGGACGGCGGACGGGTGGCGGCACACGAAATCATGCTCGGTACGCCTGCGATCCGGAACCTGATAC
GGGAAAACAAAGTGGCGCAGATGGCGTCTGTCATCCAGACCGGCGCAGCGATCGGCATGCAGACATTGGACATGAGTGTG
AAACGCTTGCAGGAGAGGGGAATCATCGACCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

65.653

99.396

0.653
  pilT Acinetobacter nosocomialis M2

65.35

99.396

0.65
  pilT Acinetobacter baumannii D1279779

65.35

99.396

0.65
  pilT Acinetobacter baumannii strain A118

65.35

99.396

0.65
  pilT Pseudomonas stutzeri DSM 10701

65.35

99.396

0.65
  pilT Acinetobacter baylyi ADP1

64.653

100

0.647
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

61.212

99.698

0.61
  pilT Vibrio cholerae strain A1552

61.212

99.698

0.61
  pilT Legionella pneumophila strain Lp02

60.486

99.396

0.601
  pilT Legionella pneumophila strain ERS1305867

60.486

99.396

0.601
  pilT Neisseria meningitidis 8013

59.394

99.698

0.592
  pilT Neisseria gonorrhoeae MS11

59.091

99.698

0.589
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.303

99.698

0.502
  pilU Acinetobacter baylyi ADP1

41.304

97.281

0.402
  pilU Vibrio cholerae strain A1552

40.123

97.885

0.393
  pilU Pseudomonas stutzeri DSM 10701

39.091

99.698

0.39