Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IE339_RS19825 Genome accession   NZ_CP061868
Coordinates   3758249..3759289 (-) Length   346 a.a.
NCBI ID   WP_242176253.1    Uniprot ID   -
Organism   Priestia koreensis strain FS-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3753249..3764289
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IE339_RS19800 (IE339_19780) - 3754009..3754650 (-) 642 WP_242170447.1 hypothetical protein -
  IE339_RS19805 (IE339_19785) pilM 3754650..3755642 (-) 993 WP_242170449.1 pilus assembly protein PilM -
  IE339_RS19810 (IE339_19790) - 3755666..3756418 (-) 753 WP_242170453.1 A24 family peptidase -
  IE339_RS19815 (IE339_19795) - 3756484..3756957 (-) 474 WP_242170456.1 type II secretion system protein -
  IE339_RS19820 (IE339_19800) - 3757041..3758249 (-) 1209 WP_242170460.1 type II secretion system F family protein -
  IE339_RS19825 (IE339_19805) pilT 3758249..3759289 (-) 1041 WP_242176253.1 type IV pilus twitching motility protein PilT Machinery gene
  IE339_RS19830 (IE339_19810) - 3759304..3760968 (-) 1665 WP_242170463.1 ATPase, T2SS/T4P/T4SS family -
  IE339_RS19835 (IE339_19815) - 3761023..3762480 (-) 1458 WP_242170466.1 VanW family protein -
  IE339_RS19840 (IE339_19820) - 3762499..3763308 (-) 810 WP_242170468.1 PRC-barrel domain-containing protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38293.05 Da        Isoelectric Point: 8.0384

>NTDB_id=485386 IE339_RS19825 WP_242176253.1 3758249..3759289(-) (pilT) [Priestia koreensis strain FS-1]
MNTQIQELMRVAFNYKASDLHLTVGVPPILRINGELKRYGKEALKPQDLEGMTKDIVPETHWDTFCDKGELDFSYGLPDV
SRFRVNAYHQRGSKSLAIRIIPTIVPTLEDLKMPPILKTIAAKPQGLVLVTGPTGSGKSTTLASMIHHMNTTMKRHIITL
EDPIEYLHRHGTSIIDQREIGFDTQSFANGLRASLRQDPDVILVGELRDLETIHTAITAAETGHLVLGTLHTSSAPSTIN
RVLDVFPPNQQIQIRIQLASVLEGIISQRLFPTSDKKGRVCATEILVNNSAIANLIRNEKVHQIPSVMQTNRASGMHTLE
TSIQDLVKAGRITEAAAQPYLQARDA

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=485386 IE339_RS19825 WP_242176253.1 3758249..3759289(-) (pilT) [Priestia koreensis strain FS-1]
ATCAATACACAAATTCAAGAACTAATGAGGGTGGCGTTTAATTATAAAGCATCTGATCTCCATTTAACCGTCGGAGTGCC
TCCGATTTTGCGAATTAATGGGGAACTAAAACGATACGGAAAGGAAGCTTTAAAGCCTCAAGACTTGGAAGGAATGACGA
AGGATATCGTGCCTGAAACGCATTGGGATACATTTTGTGATAAAGGTGAGCTAGACTTTTCATACGGTCTTCCAGACGTT
TCGAGGTTTCGTGTCAATGCTTATCATCAGCGGGGGTCAAAGTCGCTAGCGATTCGAATTATTCCAACTATTGTTCCAAC
ACTTGAAGATTTAAAAATGCCGCCGATATTAAAAACAATAGCAGCAAAACCTCAAGGTCTTGTTCTTGTGACAGGACCGA
CGGGAAGTGGGAAATCAACCACCCTTGCTTCTATGATTCATCACATGAACACGACGATGAAACGGCACATCATCACCCTT
GAAGATCCGATTGAGTATTTGCATCGACACGGAACGAGTATTATTGATCAGCGAGAAATTGGTTTTGACACACAAAGCTT
TGCAAACGGCCTTCGTGCTTCTCTCCGTCAGGATCCTGACGTTATTTTAGTCGGAGAGCTTCGTGATTTAGAAACGATTC
ATACGGCGATTACAGCTGCTGAGACGGGTCACTTAGTACTTGGAACGCTACATACCTCAAGTGCACCTTCAACAATTAAC
CGTGTGCTTGACGTATTTCCACCAAATCAGCAGATTCAAATTCGAATTCAGCTCGCCTCAGTACTAGAAGGCATTATTTC
ACAACGTCTGTTTCCAACGTCTGATAAAAAAGGGCGGGTCTGTGCAACGGAGATTTTGGTGAATAATTCGGCAATCGCCA
ACCTTATTCGTAACGAAAAGGTTCATCAAATACCGAGTGTGATGCAGACGAATCGTGCTTCGGGAATGCATACGTTAGAG
ACATCGATTCAAGATCTCGTGAAAGCAGGGCGTATAACCGAAGCTGCTGCTCAACCTTATCTACAAGCGAGGGATGCTTA
G

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(82-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.28

95.087

0.497
  pilT Vibrio cholerae strain A1552

48.521

97.688

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.521

97.688

0.474
  pilT Neisseria meningitidis 8013

47.522

99.133

0.471
  pilT Neisseria gonorrhoeae MS11

47.522

99.133

0.471
  pilT Acinetobacter nosocomialis M2

47.941

98.266

0.471
  pilT Acinetobacter baumannii D1279779

47.941

98.266

0.471
  pilT Acinetobacter baumannii strain A118

47.941

98.266

0.471
  pilT Pseudomonas stutzeri DSM 10701

46.471

98.266

0.457
  pilT Pseudomonas aeruginosa PAK

46.407

96.532

0.448
  pilT Acinetobacter baylyi ADP1

46.646

94.798

0.442
  pilT Legionella pneumophila strain Lp02

45.509

96.532

0.439
  pilT Legionella pneumophila strain ERS1305867

45.509

96.532

0.439
  pilU Acinetobacter baylyi ADP1

40.12

96.532

0.387
  pilU Vibrio cholerae strain A1552

41.231

93.931

0.387
  pilU Pseudomonas stutzeri DSM 10701

40

95.376

0.382