Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   IEZ30_RS24620 Genome accession   NZ_CP061849
Coordinates   431033..431452 (+) Length   139 a.a.
NCBI ID   WP_118266435.1    Uniprot ID   -
Organism   Chromobacterium haemolyticum strain Bb2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 426033..436452
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IEZ30_RS01885 (IEZ30_01885) proB 426565..427683 (-) 1119 WP_043592290.1 glutamate 5-kinase -
  IEZ30_RS01890 (IEZ30_01890) - 427883..429385 (-) 1503 WP_141113105.1 hypothetical protein -
  IEZ30_RS01895 (IEZ30_01895) - 429487..430839 (-) 1353 WP_118266433.1 adenosylmethionine--8-amino-7-oxononanoate transaminase -
  IEZ30_RS24620 (IEZ30_01900) pilA2 431033..431452 (+) 420 WP_118266435.1 pilin Machinery gene
  IEZ30_RS01905 (IEZ30_01905) pilA 431465..431902 (+) 438 WP_118266438.1 pilin Machinery gene
  IEZ30_RS01910 (IEZ30_01910) - 431853..433775 (+) 1923 WP_191186269.1 Wzy polymerase domain-containing protein -
  IEZ30_RS01915 (IEZ30_01915) - 433851..435635 (+) 1785 WP_191186270.1 O-antigen ligase family protein -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14334.31 Da        Isoelectric Point: 5.6543

>NTDB_id=485140 IEZ30_RS24620 WP_118266435.1 431033..431452(+) (pilA2) [Chromobacterium haemolyticum strain Bb2]
MRKAAGFTLIELMIVVAIIGILAAIAIPAYQNYVVRAKVAEGLTYADQAKTSVSEYYLAGNTWPSTNASAGLPDTLAGNY
VSNVQVGVSSGVSTVTVLFNSSVVSGLTVVFTPVASSGTVAWSCSTDAAYAQYVPSSCH

Nucleotide


Download         Length: 420 bp        

>NTDB_id=485140 IEZ30_RS24620 WP_118266435.1 431033..431452(+) (pilA2) [Chromobacterium haemolyticum strain Bb2]
ATGAGGAAGGCGGCTGGGTTTACCTTGATTGAACTAATGATTGTTGTCGCCATTATTGGTATTTTGGCGGCGATTGCGAT
TCCGGCTTATCAGAACTATGTGGTTCGTGCCAAGGTGGCGGAAGGCTTGACCTATGCGGATCAGGCCAAAACCTCGGTGT
CAGAATACTATCTGGCTGGCAATACTTGGCCAAGCACTAATGCTAGTGCAGGTTTACCTGATACTTTGGCCGGAAACTAT
GTCAGCAATGTACAGGTTGGAGTTAGTAGTGGAGTCAGTACTGTGACCGTGTTGTTTAATAGCAGTGTGGTCTCTGGTTT
GACTGTGGTCTTTACCCCTGTAGCTAGTAGTGGTACTGTTGCGTGGTCGTGCTCTACCGATGCGGCTTATGCTCAATATG
TGCCTTCGTCTTGCCATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

49.286

100

0.496
  comP Acinetobacter baylyi ADP1

45.638

100

0.489
  pilA2 Legionella pneumophila strain ERS1305867

48.571

100

0.489
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

32.447

100

0.439
  pilE Neisseria gonorrhoeae MS11

38.71

100

0.432
  pilA Pseudomonas aeruginosa PAK

36.076

100

0.41
  pilE Neisseria gonorrhoeae strain FA1090

37.013

100

0.41
  pilA Ralstonia pseudosolanacearum GMI1000

45.6

89.928

0.41
  pilA Acinetobacter nosocomialis M2

39.286

100

0.396
  pilA/pilA1 Eikenella corrodens VA1

36.486

100

0.388
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.063

100

0.381
  pilA Vibrio parahaemolyticus RIMD 2210633

41.6

89.928

0.374
  pilA Vibrio cholerae C6706

35.862

100

0.374
  pilA Vibrio cholerae strain A1552

35.862

100

0.374
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

35.862

100

0.374