NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	IEZ30_RS01905	Genome accession	NZ_CP061849
Coordinates	431465..431902 (+)	Length	145 a.a.
NCBI ID	WP_118266438.1	Uniprot ID	-
Organism	Chromobacterium haemolyticum strain Bb2
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 426465..436902

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IEZ30_RS01885 (IEZ30_01885)	proB	426565..427683 (-)	1119	WP_043592290.1	glutamate 5-kinase	-
IEZ30_RS01890 (IEZ30_01890)	-	427883..429385 (-)	1503	WP_141113105.1	hypothetical protein	-
IEZ30_RS01895 (IEZ30_01895)	-	429487..430839 (-)	1353	WP_118266433.1	adenosylmethionine--8-amino-7-oxononanoate transaminase	-
IEZ30_RS24620 (IEZ30_01900)	pilA2	431033..431452 (+)	420	WP_118266435.1	pilin	Machinery gene
IEZ30_RS01905 (IEZ30_01905)	pilA	431465..431902 (+)	438	WP_118266438.1	pilin	Machinery gene
IEZ30_RS01910 (IEZ30_01910)	-	431853..433775 (+)	1923	WP_191186269.1	Wzy polymerase domain-containing protein	-
IEZ30_RS01915 (IEZ30_01915)	-	433851..435635 (+)	1785	WP_191186270.1	O-antigen ligase family protein	-
IEZ30_RS01920 (IEZ30_01920)	rpoH	435708..436571 (-)	864	WP_019102027.1	RNA polymerase sigma factor RpoH	-

Sequence

Protein

Download Length: 145 a.a. Molecular weight: 14896.85 Da Isoelectric Point: 8.4688

                                >NTDB_id=485115 IEZ30_RS01905 WP_118266438.1 431465..431902(+) (pilA) [Chromobacterium haemolyticum strain Bb2]

MQKSQQGFTLIELMIVVAIIGILAAIAIPAYQDYTKRARVSEGLSLASAAKTAVAEYYASNGSFPSTNTSAGLQTSISGN

SVTSVLVGTSGVITVSYSTTLIDSTKNQLTLTPVASNGSIVWTCGASGTNALNSQWLPANCRAGN

Nucleotide

Download Length: 438 bp

                                >NTDB_id=485115 IEZ30_RS01905 WP_118266438.1 431465..431902(+) (pilA) [Chromobacterium haemolyticum strain Bb2]

ATGCAAAAATCCCAACAAGGTTTTACTCTTATCGAACTGATGATAGTGGTGGCTATTATTGGTATTCTCGCCGCGATTGC

TATTCCCGCTTATCAGGACTATACCAAACGGGCTAGGGTGTCAGAGGGGCTAAGCTTGGCTTCTGCAGCCAAAACTGCAG

TGGCAGAGTACTATGCTTCAAATGGTTCTTTCCCCTCTACAAATACTTCAGCTGGCTTGCAAACATCAATTAGTGGTAAC

TCAGTGACTAGTGTGCTTGTTGGTACAAGCGGTGTCATTACGGTTTCATATAGCACTACTTTGATTGACTCCACCAAAAA

TCAATTGACGCTGACACCTGTTGCATCGAATGGGTCGATTGTTTGGACATGTGGAGCGTCTGGGACTAATGCCCTTAACT

CTCAGTGGTTGCCAGCTAACTGCCGTGCCGGCAACTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-142)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Ralstonia pseudosolanacearum GMI1000	45.399	100	0.51
pilA2	Legionella pneumophila str. Paris	51.079	95.862	0.49
pilA2	Legionella pneumophila strain ERS1305867	51.079	95.862	0.49
pilE	Neisseria gonorrhoeae MS11	43.75	100	0.483
pilE	Neisseria gonorrhoeae strain FA1090	44.025	100	0.483
comP	Acinetobacter baylyi ADP1	45.946	100	0.469
pilA/pilA1	Eikenella corrodens VA1	40.506	100	0.441
pilA	Haemophilus influenzae 86-028NP	41.549	97.931	0.407
pilA/pilAII	Pseudomonas stutzeri DSM 10701	40.278	99.31	0.4
pilA	Haemophilus influenzae Rd KW20	40	100	0.4
pilA	Vibrio cholerae C6706	37.5	100	0.393
pilA	Vibrio cholerae strain A1552	37.5	100	0.393
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	37.5	100	0.393
pilA	Vibrio parahaemolyticus RIMD 2210633	43.75	88.276	0.386
pilA	Pseudomonas aeruginosa PAK	34.641	100	0.366

Detailed information NTG0485115