Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H2514_RS01470 Genome accession   NZ_CP061847
Coordinates   351611..352717 (-) Length   368 a.a.
NCBI ID   WP_036191796.1    Uniprot ID   A0A0A0ERT8
Organism   Lysobacter sp. CW239     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 346611..357717
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2514_RS01440 (H2514_01440) - 347030..348232 (-) 1203 WP_052103249.1 (Fe-S)-binding protein -
  H2514_RS01445 (H2514_01445) rplM 348403..348831 (+) 429 WP_036191783.1 50S ribosomal protein L13 -
  H2514_RS01450 (H2514_01450) rpsI 348843..349235 (+) 393 WP_036191786.1 30S ribosomal protein S9 -
  H2514_RS01460 (H2514_01460) - 349439..350425 (+) 987 WP_036191790.1 fumarylacetoacetate hydrolase family protein -
  H2514_RS01465 (H2514_01465) maiA 350535..351200 (+) 666 WP_036191793.1 maleylacetoacetate isomerase -
  H2514_RS01470 (H2514_01470) pilU 351611..352717 (-) 1107 WP_036191796.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H2514_RS01475 (H2514_01475) - 352891..353193 (+) 303 WP_052103250.1 DUF4398 domain-containing protein -
  H2514_RS01480 (H2514_01480) - 353190..353822 (+) 633 WP_052103251.1 hypothetical protein -
  H2514_RS01485 (H2514_01485) - 354211..354429 (+) 219 WP_036191802.1 YdcH family protein -
  H2514_RS01490 (H2514_01490) - 354572..355948 (+) 1377 WP_036191804.1 pyridoxal-phosphate dependent enzyme -
  H2514_RS01495 (H2514_01495) - 356131..357309 (+) 1179 WP_036191807.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40857.07 Da        Isoelectric Point: 6.4438

>NTDB_id=485072 H2514_RS01470 WP_036191796.1 351611..352717(-) (pilU) [Lysobacter sp. CW239]
MDIGYFLKLMTEKNASDMFLSTGAPVYIKIEGRLHPLGNTGLPPGMVKKIAYSLMGEGQVPQFERDLELNMAYALPDTGR
FRVNVFKQRGEVGMVIRAIRSVIPSIEELQLPQVLKNIILEPRGLVLIVGSTGSGKSTTLASMIDHRNSNVSGHILTIED
PIEYLHRHKRSLVNQREVGLDTHAFQNALKNAMREAPDVILIGEILDQTTMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPEGAHKNVLMNLALNLQAVISQRLVIGVDGRRLPAAEVLINTPHIRDLMRRGQVHEIKHAMEQSLEAGMETFDQCL
FRLQKEGKIDIEEALRAADSRDGLALKFRLSEGAGAEHDPYAEVFSTQ

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=485072 H2514_RS01470 WP_036191796.1 351611..352717(-) (pilU) [Lysobacter sp. CW239]
ATGGACATCGGCTATTTCCTGAAGCTGATGACGGAAAAGAATGCGTCGGACATGTTCTTGAGCACCGGCGCGCCGGTGTA
CATCAAGATCGAGGGCCGGCTGCATCCACTGGGCAATACCGGCCTGCCGCCGGGGATGGTCAAGAAGATCGCCTATTCGC
TGATGGGCGAGGGCCAGGTGCCGCAGTTCGAGCGCGACCTGGAATTGAACATGGCCTATGCGCTGCCCGACACCGGTCGT
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAGGTCGGCATGGTGATCCGTGCGATCCGCAGCGTGATCCCGTCGATCGA
GGAGCTGCAACTGCCGCAGGTGCTCAAGAACATCATCCTGGAGCCGCGCGGACTGGTGCTGATCGTCGGCTCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGTCGATGATCGACCACCGCAACAGCAACGTCTCCGGCCACATCCTCACCATCGAGGAC
CCGATCGAATACCTGCACCGGCACAAGCGCTCGCTGGTCAACCAGCGCGAGGTCGGCCTGGATACCCACGCCTTCCAGAA
CGCGCTGAAGAACGCGATGCGCGAGGCGCCCGACGTGATCCTGATCGGCGAGATCCTCGATCAGACCACCATGGAAGCGG
CCATCGCGTTCGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACTCCAACAATGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCCGAGGGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCAGGCGGTGATCTCGCAGCG
GCTGGTGATCGGCGTTGATGGCCGCCGGCTGCCGGCCGCCGAGGTGCTGATCAACACCCCGCATATCCGCGACCTGATGC
GCCGCGGCCAGGTCCATGAGATCAAGCACGCGATGGAGCAGTCGCTGGAAGCGGGGATGGAGACCTTCGACCAGTGCCTG
TTCCGGCTGCAGAAGGAAGGCAAGATCGATATCGAGGAGGCGTTGCGTGCCGCCGATTCGCGTGACGGGCTGGCGTTGAA
ATTCCGCCTGTCCGAAGGCGCCGGCGCCGAGCACGACCCGTATGCCGAGGTGTTCTCGACCCAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A0ERT8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.418

97.554

0.492
  pilU Acinetobacter baylyi ADP1

48.179

97.011

0.467
  pilU Vibrio cholerae strain A1552

48.023

96.196

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.205

99.185

0.448
  pilT Pseudomonas aeruginosa PAK

42.899

91.848

0.394
  pilT Acinetobacter baumannii strain A118

42.604

91.848

0.391
  pilT Acinetobacter nosocomialis M2

42.604

91.848

0.391
  pilT Acinetobacter baumannii D1279779

42.604

91.848

0.391
  pilT Pseudomonas stutzeri DSM 10701

42.308

91.848

0.389
  pilT Acinetobacter baylyi ADP1

42.059

92.391

0.389
  pilT Vibrio cholerae strain A1552

42.216

90.761

0.383
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.216

90.761

0.383
  pilT Neisseria gonorrhoeae MS11

40.058

94.293

0.378
  pilT Neisseria meningitidis 8013

40.058

94.293

0.378
  pilT Legionella pneumophila strain Lp02

41.124

91.848

0.378
  pilT Legionella pneumophila strain ERS1305867

41.124

91.848

0.378