Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   IDM46_RS02315 Genome accession   NZ_CP061714
Coordinates   492698..493813 (-) Length   371 a.a.
NCBI ID   WP_182822918.1    Uniprot ID   A0A7X0QLF5
Organism   Luteimonas sp. MC1825     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 487698..498813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IDM46_RS02275 (IDM46_02275) sugE 487966..488283 (+) 318 WP_182822931.1 quaternary ammonium compound efflux SMR transporter SugE -
  IDM46_RS02280 (IDM46_02280) coq7 488297..488938 (-) 642 WP_182822929.1 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase -
  IDM46_RS02285 (IDM46_02285) rplM 489125..489553 (+) 429 WP_182822926.1 50S ribosomal protein L13 -
  IDM46_RS02290 (IDM46_02290) rpsI 489556..489948 (+) 393 WP_182822924.1 30S ribosomal protein S9 -
  IDM46_RS02300 (IDM46_02300) - 490141..490884 (+) 744 WP_221441764.1 2OG-Fe dioxygenase family protein -
  IDM46_RS02305 (IDM46_02305) - 490961..491950 (+) 990 WP_185114698.1 fumarylacetoacetate hydrolase family protein -
  IDM46_RS02310 (IDM46_02310) maiA 492028..492693 (+) 666 WP_182822920.1 maleylacetoacetate isomerase -
  IDM46_RS02315 (IDM46_02315) pilU 492698..493813 (-) 1116 WP_182822918.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IDM46_RS02320 (IDM46_02320) - 493966..494295 (+) 330 WP_223878004.1 DUF4398 domain-containing protein -
  IDM46_RS02325 (IDM46_02325) - 494292..494924 (+) 633 WP_185114699.1 hypothetical protein -
  IDM46_RS02330 (IDM46_02330) - 495268..495486 (+) 219 WP_182822911.1 YdcH family protein -
  IDM46_RS02335 (IDM46_02335) - 495639..497015 (+) 1377 WP_182822909.1 pyridoxal-phosphate dependent enzyme -
  IDM46_RS02340 (IDM46_02340) - 497087..498283 (+) 1197 WP_185114700.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41270.40 Da        Isoelectric Point: 5.9387

>NTDB_id=484335 IDM46_RS02315 WP_182822918.1 492698..493813(-) (pilU) [Luteimonas sp. MC1825]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMALSLADSGR
FRVNVFKQRGEVGMVIRAIRSTIPSIEELQLPSVLKDIIMAPRGLVLIVGSTGSGKSTTLASMIDYRNSTTPGHILTIED
PIEYLHRHKKSIVNQREVGIDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHICLATLHSNNADQTIERI
LNFFPEAAHKNVLMNLALNLRSVVSQRLVVGTDGRRMPATEVLINTPMIRDLLRRGQVHEIKQAMEESLEEGMESFDQCL
FRLFKDGRIERETALRAADSRDGLDLKFRLSEGGDEHDPYADVFDTGHASH

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=484335 IDM46_RS02315 WP_182822918.1 492698..493813(-) (pilU) [Luteimonas sp. MC1825]
ATGGACATCGGCTATTTCCTCAAGCTGATGACGGAAAAGAATGCCTCCGACATGTTCCTGACCACCGGTGCGCCGGTGTA
CATCAAGGTCGAAGGCAAGCTTTACCCGCTCGGCAATACCGGCCTGCCTCCCGGCATGGTGAAGAAGATCGCCTACTCGC
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAGCTCAACATGGCGCTGTCGCTGGCCGACTCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAGGTCGGCATGGTGATCCGTGCGATCCGCAGCACCATCCCCTCGATCGA
GGAGCTGCAGCTGCCGTCCGTGCTGAAGGACATCATCATGGCCCCGCGTGGCCTGGTGCTGATCGTCGGGTCCACCGGCT
CGGGCAAGTCGACCACGCTGGCGTCGATGATCGATTACCGCAACAGCACCACCCCGGGGCACATCCTCACCATCGAGGAT
CCGATCGAATACCTGCACCGGCACAAGAAGTCGATCGTCAACCAGCGCGAGGTCGGCATCGATACCCACGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTCATCCTGATCGGCGAGATCCTCGACGCCACCACGATGGAGGCCG
CGATCGCGTTCGCCGAGACCGGCCACATCTGCCTGGCCACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAATTTCTTCCCCGAGGCGGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGTTCGGTGGTCTCGCAGCG
CCTTGTCGTGGGCACCGACGGTCGCCGGATGCCGGCCACCGAAGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGATCAAGCAGGCGATGGAGGAGTCGCTGGAGGAAGGCATGGAGTCGTTCGACCAGTGCCTG
TTCCGGCTGTTCAAGGACGGCCGCATCGAGCGCGAGACCGCGCTGCGGGCAGCCGACTCGCGCGACGGCCTGGACCTCAA
GTTCCGCCTGTCCGAAGGCGGCGACGAGCACGACCCCTACGCCGACGTCTTCGACACCGGCCACGCCTCGCACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7X0QLF5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.532

96.765

0.499
  pilU Vibrio cholerae strain A1552

48.315

95.957

0.464
  pilU Acinetobacter baylyi ADP1

47.486

96.496

0.458
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.221

92.722

0.429
  pilT Pseudomonas aeruginosa PAK

42.604

91.105

0.388
  pilT Acinetobacter baylyi ADP1

41.176

91.644

0.377
  pilT Acinetobacter baumannii strain A118

41.124

91.105

0.375
  pilT Acinetobacter nosocomialis M2

41.124

91.105

0.375
  pilT Acinetobacter baumannii D1279779

41.124

91.105

0.375
  pilT Pseudomonas stutzeri DSM 10701

41.124

91.105

0.375
  pilT Legionella pneumophila strain Lp02

41.124

91.105

0.375
  pilT Legionella pneumophila strain ERS1305867

41.124

91.105

0.375
  pilT Neisseria gonorrhoeae MS11

39.769

93.531

0.372
  pilT Neisseria meningitidis 8013

39.769

93.531

0.372
  pilT Vibrio cholerae strain A1552

38.905

93.531

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

38.905

93.531

0.364