Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IC789_RS04465 Genome accession   NZ_CP061592
Coordinates   932229..933266 (-) Length   345 a.a.
NCBI ID   WP_019456823.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain AS60     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 927229..938266
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC789_RS04435 (IC789_04430) - 928012..928467 (+) 456 WP_004703295.1 bacteriohemerythrin -
  IC789_RS04440 (IC789_04435) - 928620..929687 (+) 1068 WP_143221137.1 hypothetical protein -
  IC789_RS04445 (IC789_04440) - 929687..930010 (+) 324 WP_019456825.1 RnfH family protein -
  IC789_RS04450 (IC789_04445) - 930066..930464 (-) 399 WP_004703289.1 outer membrane protein assembly factor BamE -
  IC789_RS04455 (IC789_04450) fur 930576..931013 (+) 438 WP_004703287.1 ferric iron uptake transcriptional regulator -
  IC789_RS04460 (IC789_04455) pilU 931083..932201 (-) 1119 WP_004703285.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IC789_RS04465 (IC789_04460) pilT 932229..933266 (-) 1038 WP_019456823.1 type IV pilus twitching motility protein PilT Machinery gene
  IC789_RS04470 (IC789_04465) - 933393..934085 (+) 693 WP_077170049.1 YggS family pyridoxal phosphate-dependent enzyme -
  IC789_RS04475 (IC789_04470) - 934192..935034 (+) 843 WP_019456821.1 undecaprenyl-diphosphate phosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38372.98 Da        Isoelectric Point: 6.6653

>NTDB_id=483399 IC789_RS04465 WP_019456823.1 932229..933266(-) (pilT) [Acinetobacter seifertii strain AS60]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMMDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KGLVSRGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=483399 IC789_RS04465 WP_019456823.1 932229..933266(-) (pilT) [Acinetobacter seifertii strain AS60]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCTTCCGATTTGCATTTATCTGCTGGCATGCCACCAAT
GATTCGTGTCGATGGTGAAGTTCGCCGTATTAACTTACCAGCCTTAGAACATAAAGATGTCCACCGCCTTGTGTACGACA
TCATGAACGATAAACAGCGCCGTGATTATGAAGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCTAATGTTGCACGT
TTCCGTGTGAACGCATTTAACCAAAACCGTGGTGCTGGCGCAGTATTTCGTACCATTCCATCTAAAGTACTCACCATGGA
AGATTTAGGCTTAGGTCAAATCTTTAAAGACATTTGTGACTATCCTCGCGGGATTGTCCTAGTTACAGGTCCAACAGGTT
CGGGTAAATCAACCACGCTTGCAGCGATGATGGACTACATTAATGAAAATCGTTATGACCATATTTTAACGGTTGAAGAC
CCAATCGAATTTGTACACCAATCAAAAAAATGCCTCATTAACCAGCGTGAAGTCCATCGTGATACACATGGCTTTAATGA
AGCACTTCGCTCAGCACTGCGTGAAGATCCTGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAAACGATTCGTTTGG
CCCTTACAGCTGCCGAAACAGGTCACTTAGTATTTGGTACGCTTCATACCACATCTGCCGCTAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTACGTGCCATGCTGTCTGAATCTTTGCAAGCCGTTATTTCGCAAAC
TCTGCTTAAAAAGAACGGCGGCGGTCGTGTGGCAGCACATGAAATCATGATTGGTATTCCTGCCATTCGTAACTTAATCC
GTGAAAACAAAGTCGCACAAATGTATTCAGCCATTCAAACTGGTGCTAACTATGGCATGACAACGCTTGACCAAAGCTTA
AAAGGTTTAGTGTCACGTGGTGTAATTAGCCCACAAACTGCACGTACTGCGGCAAAACAGCCTGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

99.42

100

0.994
  pilT Acinetobacter baumannii D1279779

99.13

100

0.991
  pilT Acinetobacter baumannii strain A118

99.13

100

0.991
  pilT Acinetobacter baylyi ADP1

88.081

99.71

0.878
  pilT Pseudomonas aeruginosa PAK

83.721

99.71

0.835
  pilT Pseudomonas stutzeri DSM 10701

83.43

99.71

0.832
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.131

97.391

0.693
  pilT Vibrio cholerae strain A1552

71.131

97.391

0.693
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388