Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IC806_RS06055 Genome accession   NZ_CP061471
Coordinates   1191382..1192437 (-) Length   351 a.a.
NCBI ID   WP_063330237.1    Uniprot ID   -
Organism   Geobacillus zalihae strain SURF-114     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1186382..1197437
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC806_RS06025 (IC806_06025) - 1186564..1187283 (-) 720 WP_081132377.1 pilus assembly protein PilO -
  IC806_RS06030 (IC806_06030) - 1187280..1187855 (-) 576 WP_081132375.1 PilN domain-containing protein -
  IC806_RS06035 (IC806_06035) pilM 1187845..1188783 (-) 939 WP_014196431.1 type IV pilus biogenesis protein PilM -
  IC806_RS06040 (IC806_06040) - 1188805..1189551 (-) 747 WP_014196432.1 A24 family peptidase -
  IC806_RS06045 (IC806_06045) - 1189580..1190098 (-) 519 WP_190305335.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  IC806_RS06050 (IC806_06050) - 1190184..1191395 (-) 1212 WP_062898840.1 type II secretion system F family protein -
  IC806_RS06055 (IC806_06055) pilT 1191382..1192437 (-) 1056 WP_063330237.1 type IV pilus twitching motility protein PilT Machinery gene
  IC806_RS06060 (IC806_06060) - 1192450..1194114 (-) 1665 WP_023633503.1 GspE/PulE family protein -
  IC806_RS06065 (IC806_06065) - 1194111..1195481 (-) 1371 WP_081132373.1 VanW family protein -
  IC806_RS06070 (IC806_06070) - 1195498..1196277 (-) 780 WP_081132371.1 PRC-barrel domain-containing protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38525.50 Da        Isoelectric Point: 6.7270

>NTDB_id=482416 IC806_RS06055 WP_063330237.1 1191382..1192437(-) (pilT) [Geobacillus zalihae strain SURF-114]
MKEKLEALLRAAFECRASDVHLTVGAPPIFRVNGELRAYGHERLHPEETEQMARSIIPAPLWQQFQETGELDFSYGIAGV
SRFRLNVFKQRSCVSLAVRLIPVRIPTLEELGLPDVLKRIAEKPHGLVLVTGPTGSGKSTTLAAMIDYMNKTMSKHIITL
EDPIEYLHKHGKSIIDQREVGFDTGSFAAGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHLVLGTLHTASAPAAIE
RMIDVFPPAQQAQIRLQLASVLVAIIAQRLFPNRHKTGRTAALEILVNNAAVANLIRNEKEHQIINIMQTSRSLGMQTME
MSVKELAADGRIDPLAAEVYLAGERGDDGAV

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=482416 IC806_RS06055 WP_063330237.1 1191382..1192437(-) (pilT) [Geobacillus zalihae strain SURF-114]
ATGAAGGAAAAGCTTGAGGCGTTATTGCGCGCGGCCTTTGAATGCCGCGCTTCCGACGTGCATTTAACCGTCGGCGCGCC
GCCGATTTTTCGCGTGAACGGGGAGTTGAGAGCTTACGGCCATGAGCGCCTGCACCCGGAGGAAACAGAGCAAATGGCGC
GATCCATCATTCCGGCGCCGCTTTGGCAGCAGTTTCAGGAAACCGGAGAGCTTGATTTTTCGTATGGCATTGCCGGCGTT
TCCCGCTTTCGCTTAAACGTGTTTAAACAGCGGTCATGCGTGTCGCTCGCCGTGCGCCTCATCCCGGTGCGCATCCCGAC
GCTGGAGGAACTTGGCCTGCCGGATGTTTTAAAGCGCATCGCCGAAAAGCCGCACGGACTCGTGCTTGTCACCGGACCGA
CCGGCAGCGGCAAATCGACGACATTGGCGGCGATGATCGACTATATGAACAAAACGATGTCGAAACATATCATCACGCTG
GAAGATCCAATCGAGTATTTGCATAAACACGGGAAATCGATCATCGACCAACGCGAGGTGGGATTCGACACTGGCAGTTT
TGCCGCCGGATTGCGCGCTGCGCTTCGTCAAGATCCAGACGTCATTTTAGTCGGGGAAATGCGCGATTTGGAAACAATCC
AAACGGCAATCACCGCCGCGGAAACGGGCCATTTAGTGCTTGGCACGCTTCATACGGCGAGCGCGCCGGCGGCGATTGAG
CGGATGATTGACGTCTTTCCGCCGGCCCAGCAGGCGCAAATCCGGCTGCAACTTGCTTCCGTGCTCGTGGCCATCATCGC
TCAGCGCTTGTTTCCAAACAGACACAAAACCGGGCGGACGGCAGCGCTGGAAATTTTAGTGAACAATGCGGCCGTCGCCA
ACTTAATCCGAAACGAAAAAGAACATCAAATCATCAACATTATGCAAACGAGCCGCAGCCTCGGCATGCAGACGATGGAA
ATGAGCGTTAAGGAGCTTGCAGCCGATGGGCGCATCGATCCGCTGGCGGCTGAAGTGTATTTGGCGGGGGAGCGTGGGGA
CGATGGCGCAGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.313

89.174

0.484
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.387

92.877

0.459
  pilT Vibrio cholerae strain A1552

49.387

92.877

0.459
  pilT Pseudomonas aeruginosa PAK

49.536

92.023

0.456
  pilT Neisseria gonorrhoeae MS11

48.182

94.017

0.453
  pilT Neisseria meningitidis 8013

48.182

94.017

0.453
  pilT Acinetobacter baylyi ADP1

48.466

92.877

0.45
  pilT Acinetobacter baumannii strain A118

47.576

94.017

0.447
  pilT Acinetobacter baumannii D1279779

47.576

94.017

0.447
  pilT Acinetobacter nosocomialis M2

47.273

94.017

0.444
  pilT Pseudomonas stutzeri DSM 10701

48.297

92.023

0.444
  pilT Legionella pneumophila strain ERS1305867

48.276

90.883

0.439
  pilT Legionella pneumophila strain Lp02

48.276

90.883

0.439
  pilU Vibrio cholerae strain A1552

40.845

100

0.413
  pilU Pseudomonas stutzeri DSM 10701

43.769

93.732

0.41
  pilU Acinetobacter baylyi ADP1

39.688

91.168

0.362