Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EHE19_RS06665 Genome accession   NZ_CP061336
Coordinates   1550370..1551422 (+) Length   350 a.a.
NCBI ID   WP_137698886.1    Uniprot ID   A0A4U7JAE1
Organism   Ruminiclostridium herbifermentans strain MA18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1545370..1556422
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EHE19_RS06660 (EHE19_006660) - 1548399..1550090 (+) 1692 WP_137698887.1 GspE/PulE family protein -
  EHE19_RS06665 (EHE19_006665) pilT 1550370..1551422 (+) 1053 WP_137698886.1 type IV pilus twitching motility protein PilT Machinery gene
  EHE19_RS06670 (EHE19_006670) - 1551428..1552645 (+) 1218 WP_137698885.1 type II secretion system F family protein -
  EHE19_RS06675 (EHE19_006675) - 1552677..1553120 (+) 444 WP_137698884.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EHE19_RS06680 (EHE19_006680) - 1553128..1553925 (+) 798 WP_137698892.1 A24 family peptidase -
  EHE19_RS06685 (EHE19_006685) pilM 1554402..1555469 (+) 1068 WP_137698883.1 type IV pilus assembly protein PilM -
  EHE19_RS06690 (EHE19_006690) - 1555515..1556123 (+) 609 WP_137698882.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38967.76 Da        Isoelectric Point: 6.6623

>NTDB_id=481922 EHE19_RS06665 WP_137698886.1 1550370..1551422(+) (pilT) [Ruminiclostridium herbifermentans strain MA18]
MLLEDLLKKAREIGASDLHITVGVPPTYRQNGRLITFDDYRLLPADTKELVRNMLNDEQWRVYEENGELDLSFSLQGMAR
FRANVYKQRGTCCAAIRIVNLTIPSFDELGIPDIVKELSKKTKGLILVTGPTGSGKSTTLASIIDMINKERDCHILTLED
PIEYLHRHNKSIVNQREIGNDSHSYAAALRAALREDPDVILVGEMRDIETIAIAVTAAETGHLVLSTLHTIGAANTIDRI
IDVFPPHQQQQIRVQLSSVIQGVISQQLIPRKDVAGRAAAVEIMVANPAIRNLIREGKTHQINSQIQMGSRFGMQTMDSS
IANLYKKGVISQEEALIHATDQENLMRFMG

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=481922 EHE19_RS06665 WP_137698886.1 1550370..1551422(+) (pilT) [Ruminiclostridium herbifermentans strain MA18]
ATGTTACTGGAAGATTTGCTAAAAAAGGCTAGAGAGATTGGAGCTTCTGATTTACATATTACAGTTGGAGTCCCACCTAC
ATACAGACAAAATGGAAGGCTTATTACATTTGATGACTATAGATTACTCCCAGCTGATACTAAAGAGTTAGTGCGAAATA
TGCTAAATGATGAACAGTGGAGGGTATATGAAGAAAATGGGGAACTGGACTTATCATTTTCTTTACAGGGAATGGCAAGG
TTCAGGGCAAATGTTTACAAACAAAGAGGTACCTGTTGTGCTGCAATAAGAATAGTAAATTTAACAATACCGTCTTTTGA
TGAACTTGGAATACCTGACATTGTAAAGGAACTGAGTAAAAAAACAAAAGGTTTGATATTGGTAACAGGGCCAACAGGCA
GCGGAAAATCAACAACATTGGCTTCCATAATAGATATGATAAATAAAGAAAGAGATTGTCATATTCTAACTTTGGAGGAT
CCTATAGAGTATCTTCATCGACATAATAAATCAATTGTTAACCAGAGGGAAATAGGAAATGATTCTCATTCATATGCAGC
AGCGCTAAGAGCTGCATTAAGAGAAGATCCGGATGTTATTTTAGTAGGTGAGATGAGAGATATTGAGACAATTGCAATAG
CTGTCACAGCTGCTGAAACAGGGCATCTTGTACTTTCAACACTGCATACCATAGGGGCTGCTAATACAATTGACAGAATA
ATTGACGTATTTCCGCCTCATCAGCAACAGCAGATTCGTGTACAACTTTCCTCAGTTATTCAAGGTGTTATATCACAGCA
GCTCATCCCACGTAAGGATGTTGCAGGTAGAGCTGCAGCAGTTGAAATAATGGTTGCCAATCCAGCAATTAGAAACCTGA
TTCGAGAAGGTAAAACCCATCAAATTAATTCACAAATACAGATGGGTTCAAGGTTTGGCATGCAGACAATGGACAGCAGC
ATTGCGAATTTATACAAAAAAGGTGTTATAAGCCAGGAAGAAGCATTAATACATGCAACTGATCAAGAAAATCTGATGCG
ATTTATGGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(49-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4U7JAE1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.156

98.857

0.506
  pilT Pseudomonas stutzeri DSM 10701

48.571

100

0.486
  pilT Acinetobacter baumannii strain A118

48.844

98.857

0.483
  pilT Acinetobacter nosocomialis M2

48.844

98.857

0.483
  pilT Acinetobacter baumannii D1279779

48.844

98.857

0.483
  pilT Pseudomonas aeruginosa PAK

48.696

98.571

0.48
  pilT Acinetobacter baylyi ADP1

48.406

98.571

0.477
  pilT Neisseria meningitidis 8013

48.116

98.571

0.474
  pilT Legionella pneumophila strain Lp02

49.552

95.714

0.474
  pilT Legionella pneumophila strain ERS1305867

49.552

95.714

0.474
  pilT Neisseria gonorrhoeae MS11

47.826

98.571

0.471
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.102

95.429

0.469
  pilT Vibrio cholerae strain A1552

49.102

95.429

0.469
  pilU Pseudomonas stutzeri DSM 10701

41.547

99.714

0.414
  pilU Acinetobacter baylyi ADP1

39.595

98.857

0.391
  pilU Vibrio cholerae strain A1552

40.299

95.714

0.386