Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IAI47_RS03600 Genome accession   NZ_CP061083
Coordinates   750312..751319 (+) Length   335 a.a.
NCBI ID   WP_003853390.1    Uniprot ID   E0LY19
Organism   Pantoea sp. MT58     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 745312..756319
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IAI47_RS03565 (IAI47_03565) trmB 745399..746118 (+) 720 WP_008925689.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  IAI47_RS03570 (IAI47_03570) - 746118..746444 (+) 327 WP_003853403.1 YggL family protein -
  IAI47_RS03575 (IAI47_03575) - 746510..747226 (+) 717 WP_003853402.1 DUF2884 domain-containing protein -
  IAI47_RS03580 (IAI47_03580) hemW 747276..748415 (-) 1140 WP_008925688.1 radical SAM family heme chaperone HemW -
  IAI47_RS03585 (IAI47_03585) - 748408..749001 (-) 594 WP_003853397.1 XTP/dITP diphosphatase -
  IAI47_RS03590 (IAI47_03590) - 749023..749577 (-) 555 WP_003853395.1 YggT family protein -
  IAI47_RS03595 (IAI47_03595) - 749590..750297 (-) 708 WP_008925687.1 YggS family pyridoxal phosphate-dependent enzyme -
  IAI47_RS03600 (IAI47_03600) pilT 750312..751319 (+) 1008 WP_003853390.1 type IV pilus twitching motility protein PilT Machinery gene
  IAI47_RS03605 (IAI47_03605) ruvX 751313..751732 (-) 420 WP_003853387.1 Holliday junction resolvase RuvX -
  IAI47_RS03610 (IAI47_03610) - 751732..752295 (-) 564 WP_033743129.1 YqgE/AlgH family protein -
  IAI47_RS03615 (IAI47_03615) gshB 752383..753327 (-) 945 WP_003853383.1 glutathione synthase -
  IAI47_RS03620 (IAI47_03620) rsmE 753415..754146 (-) 732 WP_008925685.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  IAI47_RS03625 (IAI47_03625) endA 754217..754921 (-) 705 WP_008925684.1 deoxyribonuclease I -
  IAI47_RS03630 (IAI47_03630) - 754997..755509 (-) 513 WP_078804940.1 SprT family zinc-dependent metalloprotease -
  IAI47_RS03635 (IAI47_03635) - 755508..755666 (+) 159 WP_003853375.1 hypothetical protein -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 36344.91 Da        Isoelectric Point: 6.8747

>NTDB_id=480496 IAI47_RS03600 WP_003853390.1 750312..751319(+) (pilT) [Pantoea sp. MT58]
MNLDEMVELSVKHNAVDLHLCSGHLPYWRRQGRLEAIPQQAVLSADWMAQFMARWLSDPQCQELASTGQLDFALSLACGQ
RLRANLFKQRNGLSLALRIIASVCPTLESLQLPAIVPTLLTQQDGLILVTGATGSGKSTTLAALVDALNGQQARHIITLE
DPIEFIHHSQQSLIQQREIGLHCSSFAQGIKAALREDPDVILLGELRDSDTIRQALTAAETGHLVLATLHTRGAAQAVDR
LVDVFPAEEKHLVRTQLAGSLKAVIAQKLVPSAAGSRIGLFELLIATPGITNLIREGKMHQIPAMLQTGAQAGMQTFEQS
RLARQADGLIKPEGQ

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=480496 IAI47_RS03600 WP_003853390.1 750312..751319(+) (pilT) [Pantoea sp. MT58]
ATGAATCTGGATGAAATGGTGGAGCTTAGTGTAAAGCATAATGCCGTCGATCTGCACCTTTGCAGCGGACATTTGCCCTA
CTGGCGACGGCAGGGACGGCTGGAGGCGATTCCACAGCAGGCCGTGCTCTCTGCTGACTGGATGGCGCAGTTTATGGCCC
GCTGGCTCAGTGACCCGCAGTGTCAGGAACTGGCGAGCACAGGGCAGCTCGATTTCGCCCTGAGCCTGGCCTGCGGCCAG
CGTCTGCGCGCAAACCTGTTTAAGCAGCGTAACGGCCTGTCACTGGCGCTGCGCATTATTGCCTCTGTGTGCCCGACGCT
GGAGAGCCTGCAACTGCCTGCTATTGTGCCGACGTTACTGACGCAGCAGGATGGGCTGATTCTGGTGACCGGTGCCACGG
GCAGCGGTAAATCTACCACGCTGGCGGCGCTGGTCGATGCGCTGAACGGCCAGCAGGCGCGCCATATCATTACGCTGGAA
GACCCGATTGAATTTATTCACCACAGCCAGCAGTCGCTGATTCAGCAGCGCGAAATCGGCCTGCACTGCAGTTCGTTCGC
CCAGGGGATAAAGGCGGCACTGCGTGAAGATCCTGACGTCATTCTGCTGGGCGAACTGCGCGACAGCGACACGATCAGAC
AGGCGCTAACGGCGGCAGAGACCGGCCATCTGGTGCTGGCAACGCTGCACACACGTGGCGCAGCACAGGCGGTAGATCGG
CTGGTGGATGTCTTCCCGGCGGAGGAGAAGCATCTGGTGCGCACTCAACTGGCAGGCAGCCTGAAAGCGGTGATTGCACA
GAAACTGGTGCCGTCTGCTGCCGGGTCGCGCATTGGACTGTTTGAGCTACTGATTGCCACGCCTGGCATCACGAATCTGA
TCAGGGAAGGCAAGATGCACCAGATCCCGGCAATGCTACAGACCGGCGCACAGGCCGGTATGCAGACGTTTGAGCAGAGC
AGGCTGGCGCGTCAGGCTGACGGGCTGATTAAGCCGGAAGGTCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E0LY19

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

47.633

100

0.481
  pilT Neisseria gonorrhoeae MS11

47.633

100

0.481
  pilT Vibrio cholerae strain A1552

48.438

95.522

0.463
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.438

95.522

0.463
  pilT Acinetobacter baumannii strain A118

44.745

99.403

0.445
  pilT Acinetobacter baumannii D1279779

44.745

99.403

0.445
  pilT Acinetobacter baylyi ADP1

45.015

98.806

0.445
  pilT Acinetobacter nosocomialis M2

44.444

99.403

0.442
  pilT Pseudomonas stutzeri DSM 10701

44.545

98.507

0.439
  pilT Pseudomonas aeruginosa PAK

43.713

99.701

0.436
  pilT Legionella pneumophila strain Lp02

44.514

95.224

0.424
  pilT Legionella pneumophila strain ERS1305867

44.514

95.224

0.424
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.32

95.224

0.403
  pilU Pseudomonas stutzeri DSM 10701

37.725

99.701

0.376
  pilU Vibrio cholerae strain A1552

38.629

95.821

0.37
  pilU Acinetobacter baylyi ADP1

37.888

96.119

0.364