Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IBZ15_RS21315 Genome accession   NZ_CP061076
Coordinates   4456743..4457765 (-) Length   340 a.a.
NCBI ID   WP_413713712.1    Uniprot ID   -
Organism   Serratia marcescens strain 4526     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4451743..4462765
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IBZ15_RS21285 (IBZ15_21220) - 4452556..4453068 (+) 513 WP_094143168.1 SprT family zinc-dependent metalloprotease -
  IBZ15_RS21290 (IBZ15_21225) endA 4453170..4453865 (+) 696 WP_044030496.1 deoxyribonuclease I -
  IBZ15_RS21295 (IBZ15_21230) rsmE 4453935..4454666 (+) 732 WP_257711412.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  IBZ15_RS21300 (IBZ15_21235) gshB 4454677..4455627 (+) 951 WP_015379045.1 glutathione synthase -
  IBZ15_RS21305 (IBZ15_21240) - 4455761..4456324 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  IBZ15_RS21310 (IBZ15_21245) ruvX 4456324..4456746 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  IBZ15_RS21315 (IBZ15_21250) pilT 4456743..4457765 (-) 1023 WP_413713712.1 type IV pilus twitching motility protein PilT Machinery gene
  IBZ15_RS21320 (IBZ15_21255) - 4457786..4458493 (+) 708 WP_413713713.1 YggS family pyridoxal phosphate-dependent enzyme -
  IBZ15_RS21325 (IBZ15_21260) proC 4458513..4459334 (+) 822 WP_413713714.1 pyrroline-5-carboxylate reductase -
  IBZ15_RS21330 (IBZ15_21265) - 4459366..4459920 (+) 555 WP_103086314.1 YggT family protein -
  IBZ15_RS21335 (IBZ15_21270) yggU 4459917..4460207 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  IBZ15_RS21340 (IBZ15_21275) - 4460225..4460818 (+) 594 WP_413713715.1 XTP/dITP diphosphatase -
  IBZ15_RS21345 (IBZ15_21280) hemW 4460811..4461953 (+) 1143 WP_413713716.1 radical SAM family heme chaperone HemW -
  IBZ15_RS21350 (IBZ15_21285) - 4461990..4462238 (-) 249 WP_004937422.1 DUF2442 domain-containing protein -
  IBZ15_RS21355 (IBZ15_21290) - 4462222..4462464 (-) 243 WP_004937418.1 DUF4160 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36504.79 Da        Isoelectric Point: 7.0999

>NTDB_id=480363 IBZ15_RS21315 WP_413713712.1 4456743..4457765(-) (pilT) [Serratia marcescens strain 4526]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQSVQTLCDELLNAQQRESLQRLGQIDLALHRPGGE
RLRANVFQQSAGISLALRHIAGQSPSLAALAAPAIVPTLLRRADGLILVTGATGSGKSTTLAAMIDEINRHQPRHVLTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPCGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGECAGEGTRG

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=480363 IBZ15_RS21315 WP_413713712.1 4456743..4457765(-) (pilT) [Serratia marcescens strain 4526]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTACGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAAGCGTGCAGACCCTCTGCGACG
AATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCAGCGGTTGGGGCAGATCGATCTGGCGTTGCACCGGCCGGGCGGGGAG
CGGCTGCGCGCCAACGTTTTTCAGCAAAGCGCGGGAATTTCTCTCGCGTTGCGGCATATCGCCGGGCAATCGCCTTCGCT
TGCCGCGCTGGCGGCGCCGGCCATCGTCCCGACGTTGCTGCGGCGCGCCGACGGATTGATCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACGTTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAAAAACCCTATGTGCGCGCCCAACTGGCGGGCAGCCTGCAAGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCTTGCGGCGGACGCGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTACAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGTGCGCAGGGGAGGGAACGAGGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.242

97.059

0.526
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.242

97.059

0.526
  pilT Acinetobacter baylyi ADP1

50.453

97.353

0.491
  pilT Acinetobacter baumannii strain A118

50.303

97.059

0.488
  pilT Acinetobacter nosocomialis M2

50.303

97.059

0.488
  pilT Acinetobacter baumannii D1279779

50.303

97.059

0.488
  pilT Neisseria meningitidis 8013

50.151

97.353

0.488
  pilT Neisseria gonorrhoeae MS11

49.849

97.353

0.485
  pilT Legionella pneumophila strain ERS1305867

49.096

97.647

0.479
  pilT Legionella pneumophila strain Lp02

49.096

97.647

0.479
  pilT Pseudomonas aeruginosa PAK

48.485

97.059

0.471
  pilT Pseudomonas stutzeri DSM 10701

48.182

97.059

0.468
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.258

100

0.453
  pilU Acinetobacter baylyi ADP1

39.628

95

0.376
  pilU Vibrio cholerae strain A1552

38.889

95.294

0.371
  pilU Pseudomonas stutzeri DSM 10701

37.963

95.294

0.362