Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H9Y09_RS06865 Genome accession   NZ_CP061002
Coordinates   1434114..1435154 (+) Length   346 a.a.
NCBI ID   WP_187771782.1    Uniprot ID   -
Organism   Phascolarctobacterium faecium strain G104     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1429114..1440154
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9Y09_RS11535 thiE 1429526..1430140 (+) 615 Protein_1351 thiamine phosphate synthase -
  H9Y09_RS11540 (H9Y09_06850) - 1430212..1431657 (-) 1446 WP_187771093.1 secretin N-terminal domain-containing protein -
  H9Y09_RS06855 (H9Y09_06855) - 1431657..1432175 (-) 519 WP_021718324.1 hypothetical protein -
  H9Y09_RS06860 (H9Y09_06860) gspE 1432416..1434086 (+) 1671 WP_021718325.1 type II secretion system ATPase GspE -
  H9Y09_RS06865 (H9Y09_06865) pilT 1434114..1435154 (+) 1041 WP_187771782.1 type IV pilus twitching motility protein PilT Machinery gene
  H9Y09_RS06870 (H9Y09_06870) - 1435176..1436390 (+) 1215 WP_125669430.1 type II secretion system F family protein -
  H9Y09_RS11885 (H9Y09_06875) - 1436527..1436748 (+) 222 WP_394799334.1 type IV pilin protein -
  H9Y09_RS06880 (H9Y09_06880) - 1436673..1436960 (+) 288 WP_187771815.1 hypothetical protein -
  H9Y09_RS06885 (H9Y09_06885) - 1437247..1437975 (+) 729 WP_262406314.1 hypothetical protein -
  H9Y09_RS11795 (H9Y09_06890) - 1438070..1438471 (-) 402 WP_021718330.1 type II secretion system protein -
  H9Y09_RS11800 (H9Y09_06895) - 1438737..1439219 (-) 483 WP_021718329.1 type II secretion system protein -
  H9Y09_RS11805 (H9Y09_06900) - 1439294..1439716 (-) 423 WP_187771094.1 type IV pilin protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38842.36 Da        Isoelectric Point: 6.3438

>NTDB_id=479385 H9Y09_RS06865 WP_187771782.1 1434114..1435154(+) (pilT) [Phascolarctobacterium faecium strain G104]
MRDAFDVNASDIHLTVGLPPMFRVNGVLRQVGEEPLRREDTYNMAMELMDDRRRTQFLDNGEADFSYEIENVCRFRVNVF
KQSNCVAAALRLISNNIPSFEDLRLPDVISDLAMLPRGLVLVTGPTGSGKSTTLAAVINKINKHRKDHVITLEDPIEYKH
NHINSIINQREVGSDTKSFANGLRAALREDPDVILVGEMRDTETIATAITAAETGHLVLATLHTQDAASTVNRIIDVFPE
HQQQQIRIQLAGNLQGIVAQQLLMNREQTGRLAAFEILVATPALRNLIREGKTHQIPSYIQTGSKFGMICMDAYLAQLVR
QNKIDRSIAEERCYDPLSLERYMHGL

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=479385 H9Y09_RS06865 WP_187771782.1 1434114..1435154(+) (pilT) [Phascolarctobacterium faecium strain G104]
ATGCGTGACGCTTTTGATGTCAACGCTTCGGATATCCATTTAACGGTAGGCTTGCCGCCAATGTTCCGTGTCAATGGCGT
GCTGCGGCAGGTGGGTGAAGAACCTCTGCGCCGTGAGGATACCTATAATATGGCCATGGAACTGATGGACGATCGGCGCC
GCACGCAGTTTCTTGATAACGGCGAAGCTGATTTTTCTTATGAAATTGAAAATGTATGCCGTTTTCGTGTTAATGTTTTT
AAGCAGAGTAACTGTGTGGCAGCAGCGCTGCGTTTGATTTCCAATAATATACCCAGCTTTGAGGACCTGCGCCTGCCTGA
CGTTATCAGCGACCTTGCCATGCTGCCGCGTGGTCTGGTATTGGTGACAGGGCCTACCGGCAGCGGTAAATCAACGACAC
TGGCGGCTGTTATCAATAAAATCAACAAACACCGCAAAGATCATGTCATCACTCTGGAAGACCCGATCGAATATAAGCAT
AACCATATCAACAGCATTATCAACCAGCGCGAAGTCGGCAGTGATACCAAAAGCTTTGCCAATGGCCTTAGGGCAGCATT
ACGTGAAGATCCTGACGTTATTCTGGTAGGCGAGATGCGTGATACTGAAACTATCGCCACCGCTATCACTGCTGCAGAAA
CCGGACATCTTGTTTTGGCAACTCTGCATACTCAGGATGCGGCATCGACAGTGAATCGTATCATCGACGTCTTTCCCGAA
CACCAGCAGCAGCAGATTCGCATTCAGCTGGCTGGCAACCTGCAGGGCATCGTTGCCCAGCAGCTGCTGATGAATCGCGA
ACAAACCGGACGTCTGGCTGCTTTTGAAATATTAGTTGCCACACCGGCGCTGCGGAACTTGATCCGCGAAGGCAAAACAC
ATCAGATACCGTCATATATCCAGACCGGTTCCAAATTCGGGATGATTTGTATGGATGCCTATCTGGCGCAGCTGGTACGT
CAAAATAAGATCGACCGCAGCATAGCGGAAGAACGCTGTTATGATCCATTATCATTAGAACGCTATATGCATGGACTATA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(70-263)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.666

98.555

0.529
  pilT Legionella pneumophila strain Lp02

52.727

95.376

0.503
  pilT Legionella pneumophila strain ERS1305867

52.727

95.376

0.503
  pilT Neisseria meningitidis 8013

51.36

95.665

0.491
  pilT Neisseria gonorrhoeae MS11

51.057

95.665

0.488
  pilT Acinetobacter baylyi ADP1

49.704

97.688

0.486
  pilT Acinetobacter nosocomialis M2

48.817

97.688

0.477
  pilT Acinetobacter baumannii D1279779

48.817

97.688

0.477
  pilT Acinetobacter baumannii strain A118

48.817

97.688

0.477
  pilT Vibrio cholerae strain A1552

51.572

91.908

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.572

91.908

0.474
  pilT Pseudomonas aeruginosa PAK

48.024

95.087

0.457
  pilT Pseudomonas stutzeri DSM 10701

47.72

95.087

0.454
  pilU Pseudomonas stutzeri DSM 10701

43.519

93.642

0.408
  pilU Vibrio cholerae strain A1552

44.164

91.618

0.405
  pilU Acinetobacter baylyi ADP1

38.855

95.954

0.373