Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NMBG2136_RS00280 Genome accession   NC_017513
Coordinates   52490..53533 (-) Length   347 a.a.
NCBI ID   WP_014580721.1    Uniprot ID   -
Organism   Neisseria meningitidis G2136     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 47490..58533
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMBG2136_RS00265 (NMBG2136_0046) - 47604..47888 (+) 285 WP_002216166.1 GIY-YIG nuclease family protein -
  NMBG2136_RS00270 (NMBG2136_0047) yccS 48665..50815 (-) 2151 WP_014580720.1 YccS family putative transporter -
  NMBG2136_RS00275 (NMBG2136_0048) - 51101..52327 (-) 1227 WP_002218483.1 PilT/PilU family type 4a pilus ATPase -
  NMBG2136_RS00280 (NMBG2136_0049) pilT 52490..53533 (-) 1044 WP_014580721.1 type IV pilus twitching motility protein PilT Machinery gene
  NMBG2136_RS00285 (NMBG2136_0050) - 53653..54339 (+) 687 WP_002239404.1 YggS family pyridoxal phosphate-dependent enzyme -
  NMBG2136_RS00290 (NMBG2136_0051) - 54339..54746 (+) 408 WP_002239400.1 hypothetical protein -
  NMBG2136_RS00295 (NMBG2136_0052) proC 54777..55586 (+) 810 WP_228372978.1 pyrroline-5-carboxylate reductase -
  NMBG2136_RS00300 (NMBG2136_0053) dksA 55731..56147 (+) 417 WP_002238569.1 RNA polymerase-binding protein DksA -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38077.59 Da        Isoelectric Point: 7.0893

>NTDB_id=47890 NMBG2136_RS00280 WP_014580721.1 52490..53533(-) (pilT) [Neisseria meningitidis G2136]
MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSAEEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVAR
FRVNAFNIGRGPAAVFRTIPSTVLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAAETGHLVFGTLHTTGAAKTVDRI
VDVFPVGEKEMVRSMLSESLTAVISQNLLKTHDGNGRVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQS
LQSLVRQGLIAPEVARRRAQNSESMSF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=47890 NMBG2136_RS00280 WP_014580721.1 52490..53533(-) (pilT) [Neisseria meningitidis G2136]
ATGCAGATTACCGACTTACTCGCCTTCGGCGCTAAAAACAAAGCATCCGACCTTCACCTGAGTTCGGGCATATCCCCTAT
GATTCGGGTTCACGGCGATATGCGGCGCATCAACCTTCCCGAAATGAGCGCGGAAGAGGTCGGTAATATGGTAACTTCGG
TGATGAACGACCACCAGCGGAAAATCTACCAGCAAAACTTGGAAGTCGACTTCTCGTTCGAACTGCCCAACGTCGCCCGA
TTCCGCGTCAACGCCTTCAACATCGGCCGCGGTCCCGCCGCCGTATTCCGCACCATTCCCAGCACCGTCTTATCGCTGGA
AGAATTGAAAGCCCCGAGCATTTTCCAAAAAATCGCAGAATCGCCGCGCGGCATGGTATTGGTTACCGGCCCTACCGGTT
CGGGCAAATCGACCACGCTTGCCGCGATGATCAACTACATCAACGAAACCCAGCCGGCACACATCCTGACCATCGAAGAC
CCGATCGAATTCGTCCACCAAAGCAAAAAATCCCTGATTAACCAGCGCGAGCTGCACCAGCACACCCTCAGCTTCGCCAA
CGCGCTGCGTTCCGCATTGCGCGAAGACCCCGACGTTATCCTTGTCGGCGAGATGCGCGACCCCGAAACCATCGGCTTGG
CACTGACCGCCGCCGAAACCGGACACTTGGTTTTCGGCACGCTGCACACGACCGGCGCAGCAAAAACCGTCGACCGTATC
GTGGACGTATTCCCGGTGGGAGAAAAAGAAATGGTGCGCTCTATGCTGTCCGAATCGCTGACCGCCGTCATCTCCCAAAA
CCTGCTGAAAACGCACGACGGCAACGGCCGTGTCGCCTCGCACGAAATCCTGATTGCCAACCCCGCCGTCCGCAACCTCA
TCCGCGAAAACAAAATCACGCAGATTAACTCCGTCCTGCAAACCGGGCAGGCGAGCGGTATGCAGACAATGGACCAATCG
CTGCAATCGCTGGTGCGCCAAGGGCTGATCGCACCGGAAGTCGCACGCAGACGCGCGCAAAACAGCGAAAGTATGAGTTT
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

99.712

100

0.997
  pilT Neisseria gonorrhoeae MS11

98.847

100

0.988
  pilT Acinetobacter baumannii D1279779

67.826

99.424

0.674
  pilT Acinetobacter baumannii strain A118

67.826

99.424

0.674
  pilT Acinetobacter nosocomialis M2

67.826

99.424

0.674
  pilT Acinetobacter baylyi ADP1

67.246

99.424

0.669
  pilT Pseudomonas stutzeri DSM 10701

66.957

99.424

0.666
  pilT Pseudomonas aeruginosa PAK

66.377

99.424

0.66
  pilT Legionella pneumophila strain Lp02

64.516

98.271

0.634
  pilT Legionella pneumophila strain ERS1305867

64.516

98.271

0.634
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.545

95.101

0.614
  pilT Vibrio cholerae strain A1552

64.545

95.101

0.614
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.644

98.847

0.53
  pilU Pseudomonas stutzeri DSM 10701

40

99.424

0.398
  pilU Vibrio cholerae strain A1552

38.551

99.424

0.383
  pilU Acinetobacter baylyi ADP1

38.15

99.712

0.38

Multiple sequence alignment