Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H8B22_RS04250 Genome accession   NZ_CP060820
Coordinates   917888..918994 (+) Length   368 a.a.
NCBI ID   WP_187712878.1    Uniprot ID   -
Organism   Agrilutibacter terrestris strain II4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 912888..923994
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H8B22_RS04230 (H8B22_04230) - 913423..914277 (-) 855 WP_187712875.1 2OG-Fe(II) oxygenase -
  H8B22_RS04235 (H8B22_04235) - 914364..915740 (-) 1377 WP_187712876.1 pyridoxal-phosphate dependent enzyme -
  H8B22_RS04240 (H8B22_04240) - 915980..916198 (-) 219 WP_187712877.1 YdcH family protein -
  H8B22_RS14735 - 916559..917740 (-) 1182 WP_208456924.1 DUF4398 domain-containing protein -
  H8B22_RS04250 (H8B22_04250) pilU 917888..918994 (+) 1107 WP_187712878.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H8B22_RS04255 (H8B22_04255) maiA 919071..919730 (-) 660 WP_187712879.1 maleylacetoacetate isomerase -
  H8B22_RS04260 (H8B22_04260) - 919843..920829 (-) 987 WP_187712880.1 fumarylacetoacetate hydrolase family protein -
  H8B22_RS04270 (H8B22_04270) rpsI 921173..921565 (-) 393 WP_187712881.1 30S ribosomal protein S9 -
  H8B22_RS04275 (H8B22_04275) rplM 921578..922006 (-) 429 WP_187712882.1 50S ribosomal protein L13 -
  H8B22_RS04280 (H8B22_04280) - 922250..923401 (+) 1152 WP_187712883.1 (Fe-S)-binding protein -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40788.18 Da        Isoelectric Point: 6.4445

>NTDB_id=478613 H8B22_RS04250 WP_187712878.1 917888..918994(+) (pilU) [Agrilutibacter terrestris strain II4]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGKLHPLGNTGLPAGMVKKIAYSLMDEGQVPIFERDLELNMALAIPEAGR
FRVNVFKQRGEVGMVIRAIRSVIPSIEELQLPQVLKDIIMAPRGLVLIVGSTGSGKSTTLAAMIDHRNTNTSGHILTIED
PIEYLHKHKKSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTLERI
LNFFHESAHKNVLMNLALNLRAVISQRLVVGIDGRRMPATEVLINTPMIRDLMRRGQVHEIKPAMEGSLEEGMESFDQCL
FRLYKQGRIEREEALKAADSRDGLDLKFRLSEGGTGEHDPYADVFVAS

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=478613 H8B22_RS04250 WP_187712878.1 917888..918994(+) (pilU) [Agrilutibacter terrestris strain II4]
ATGGACATCGGCTATTTCCTGAAGCTGATGACGGAGAAGAACGCGTCGGACATGTTCCTGACCACGGGCGCTCCTGTGTA
CATCAAGGTCGAGGGCAAGCTGCATCCCCTGGGCAACACCGGCCTGCCGGCCGGCATGGTGAAGAAGATCGCCTACTCGC
TGATGGACGAGGGGCAAGTACCCATCTTCGAGCGTGACCTCGAGCTGAACATGGCCCTGGCCATCCCCGAGGCCGGCCGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAAGTCGGCATGGTGATCCGCGCGATCCGCAGCGTGATCCCGTCGATCGA
GGAGCTGCAGCTGCCGCAGGTGCTCAAGGACATCATCATGGCCCCGCGCGGCCTGGTGCTGATCGTCGGCTCCACCGGTT
CGGGCAAGTCGACCACGCTCGCGGCGATGATCGACCACCGCAATACGAATACATCGGGGCACATCCTCACCATCGAGGAT
CCGATCGAATACCTGCACAAGCACAAGAAATCGATCGTCAACCAGCGCGAAGTCGGCCTGGACACCCACGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGCGAGGCGCCCGACGTCATCCTGATCGGCGAAATCCTCGACGCGACCACGATGGAAGCGG
CGATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCGACGCTGCACTCGAACAACGCTGACCAGACACTGGAGCGCATC
CTCAACTTCTTCCACGAATCCGCGCACAAGAACGTGCTGATGAACCTCGCGCTGAACCTGCGCGCGGTCATCTCGCAGCG
CCTGGTGGTCGGCATCGACGGCCGCCGCATGCCGGCGACCGAAGTGCTGATCAACACGCCGATGATCCGCGACCTGATGC
GGCGCGGCCAGGTCCACGAGATCAAGCCGGCGATGGAGGGCTCGCTGGAGGAAGGCATGGAGAGCTTCGACCAGTGCCTG
TTCCGCCTGTACAAGCAGGGCAGGATCGAGCGCGAGGAAGCCCTCAAGGCCGCCGACTCGCGCGATGGCCTGGACCTCAA
GTTCCGCCTGTCCGAAGGCGGCACCGGCGAACACGACCCGTACGCGGACGTGTTCGTCGCGAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.811

97.554

0.505
  pilU Acinetobacter baylyi ADP1

49.435

96.196

0.476
  pilU Vibrio cholerae strain A1552

48.305

96.196

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.313

92.12

0.427
  pilT Pseudomonas aeruginosa PAK

42.604

91.848

0.391
  pilT Acinetobacter baumannii strain A118

42.012

91.848

0.386
  pilT Acinetobacter nosocomialis M2

42.012

91.848

0.386
  pilT Acinetobacter baumannii D1279779

42.012

91.848

0.386
  pilT Acinetobacter baylyi ADP1

41.471

92.391

0.383
  pilT Pseudomonas stutzeri DSM 10701

41.42

91.848

0.38
  pilT Vibrio cholerae strain A1552

40.058

94.293

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.058

94.293

0.378
  pilT Neisseria gonorrhoeae MS11

39.769

94.293

0.375
  pilT Neisseria meningitidis 8013

39.769

94.293

0.375
  pilT Legionella pneumophila strain Lp02

40.237

91.848

0.37
  pilT Legionella pneumophila strain ERS1305867

40.237

91.848

0.37