Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IAQ69_RS03280 Genome accession   NZ_CP060811
Coordinates   707321..708358 (-) Length   345 a.a.
NCBI ID   WP_004783232.1    Uniprot ID   A0A2L0I6Y4
Organism   Acinetobacter variabilis strain XM9F202-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 702321..713358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IAQ69_RS03250 (IAQ69_03250) - 703031..703486 (+) 456 WP_004783243.1 bacteriohemerythrin -
  IAQ69_RS03255 (IAQ69_03255) - 703646..704725 (+) 1080 WP_166137951.1 hypothetical protein -
  IAQ69_RS03260 (IAQ69_03260) - 704725..705054 (+) 330 WP_005221123.1 RnfH family protein -
  IAQ69_RS03265 (IAQ69_03265) - 705158..705556 (-) 399 WP_004783238.1 outer membrane protein assembly factor BamE -
  IAQ69_RS03270 (IAQ69_03270) fur 705669..706106 (+) 438 WP_004783237.1 ferric iron uptake transcriptional regulator -
  IAQ69_RS03275 (IAQ69_03275) pilU 706172..707293 (-) 1122 WP_004783234.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IAQ69_RS03280 (IAQ69_03280) pilT 707321..708358 (-) 1038 WP_004783232.1 type IV pilus twitching motility protein PilT Machinery gene
  IAQ69_RS03285 (IAQ69_03285) - 708482..709171 (+) 690 WP_179992933.1 YggS family pyridoxal phosphate-dependent enzyme -
  IAQ69_RS03290 (IAQ69_03290) - 709203..710309 (-) 1107 WP_200229836.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38251.85 Da        Isoelectric Point: 6.5056

>NTDB_id=478528 IAQ69_RS03280 WP_004783232.1 707321..708358(-) (pilT) [Acinetobacter variabilis strain XM9F202-2]
MDITELLAFSAKNGASDLHLSAGLPPMIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDYEENLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTIEDLGMGQIFKDICEYPRGIVLVTGPTGSGKSTTLAAMLDYINDNRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSKGIISPQVARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=478528 IAQ69_RS03280 WP_004783232.1 707321..708358(-) (pilT) [Acinetobacter variabilis strain XM9F202-2]
ATGGATATTACAGAACTGTTAGCCTTTTCTGCTAAAAATGGTGCGTCGGATTTACACTTATCAGCAGGTTTACCACCCAT
GATTCGTGTGGACGGGGAAGTACGCCGCATCAACCTGCCTGCACTAGAACATAAAGAAGTACACAAACTAGTGTACGACA
TCATGAACGACAAGCAACGCCGTGATTATGAAGAAAATCTGGAAACTGACTTCTCTTTTGAAGTTCCAGGCGTAGCCCGT
TTCCGTGTGAACGCATTTAACCAGAACCGCGGTGCAGGCGCAGTATTCCGTACCATTCCATCTAAAGTACTGACTATTGA
AGATCTAGGCATGGGTCAGATCTTTAAAGATATCTGTGAATATCCACGTGGTATTGTACTGGTGACAGGACCAACCGGTT
CGGGTAAATCAACGACGCTGGCGGCAATGCTGGACTATATCAATGACAACCGCTATGACCATATCCTGACCGTCGAAGAT
CCAATCGAATTTGTACACCAATCAAAAAAATGTCTGATCAACCAGCGTGAAGTACATCGTGATACGCATGGATTTAATGA
AGCATTACGTTCTGCATTACGTGAAGACCCGGATATTATTCTGGTCGGTGAGATGCGTGACCTTGAAACCATCCGTCTGG
CACTCACTGCTGCGGAAACCGGTCACTTGGTCTTCGGTACCCTGCATACCACCTCAGCGGCAAAAACCATTGACCGTGTG
ATTGACGTGTTCCCTGCTGAAGAAAAAGACATGGTGCGTGCCATGTTGTCAGAATCGCTGCAAGCTGTTATTTCGCAAAC
CCTGTTGAAGAAAAATGGGGGGGGGCGTGTGGCTGCACATGAAATCATGATCGGTATTCCAGCCATCCGTAACCTGATCC
GTGAAAACAAAGTGGCACAGATGTACTCTGCGATTCAAACAGGTGCCAACTATGGCATGACCACACTGGATCAAAGCCTG
AAAACGCTGGTATCGAAAGGCATTATCAGTCCGCAAGTCGCCCGTACTGCAGCAAAACAGCCTGAATCATTCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L0I6Y4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

95.652

100

0.957
  pilT Acinetobacter baumannii D1279779

95.362

100

0.954
  pilT Acinetobacter baumannii strain A118

95.362

100

0.954
  pilT Acinetobacter baylyi ADP1

87.5

99.71

0.872
  pilT Pseudomonas aeruginosa PAK

84.593

99.71

0.843
  pilT Pseudomonas stutzeri DSM 10701

84.302

99.71

0.841
  pilT Legionella pneumophila strain Lp02

74.419

99.71

0.742
  pilT Legionella pneumophila strain ERS1305867

74.419

99.71

0.742
  pilT Neisseria meningitidis 8013

68.986

100

0.69
  pilT Neisseria gonorrhoeae MS11

68.406

100

0.684
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.88

96.232

0.672
  pilT Vibrio cholerae strain A1552

69.88

96.232

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Vibrio cholerae strain A1552

41.003

98.261

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.088

95.942

0.394
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388