Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H9L06_RS05655 Genome accession   NZ_CP060716
Coordinates   1168486..1169538 (-) Length   350 a.a.
NCBI ID   WP_281381725.1    Uniprot ID   -
Organism   Leucobacter denitrificans strain KACC 14055     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1163486..1174538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9L06_RS05630 (H9L06_05630) - 1165538..1166131 (-) 594 WP_187556216.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  H9L06_RS05635 (H9L06_05635) - 1166128..1166556 (-) 429 WP_187556217.1 hypothetical protein -
  H9L06_RS05640 (H9L06_05640) - 1166678..1167061 (-) 384 WP_223165225.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  H9L06_RS05645 (H9L06_05645) - 1167243..1167467 (-) 225 WP_187556218.1 type II secretion system F family protein -
  H9L06_RS05650 (H9L06_05650) - 1167452..1168483 (-) 1032 WP_187556219.1 type II secretion system F family protein -
  H9L06_RS05655 (H9L06_05655) pilT 1168486..1169538 (-) 1053 WP_281381725.1 type IV pilus twitching motility protein PilT Machinery gene
  H9L06_RS05660 (H9L06_05660) - 1169643..1171232 (-) 1590 WP_246454518.1 ATPase, T2SS/T4P/T4SS family -
  H9L06_RS05665 (H9L06_05665) - 1171291..1172196 (-) 906 WP_187556220.1 bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase -
  H9L06_RS05670 (H9L06_05670) glyA 1172201..1173484 (-) 1284 WP_187556221.1 serine hydroxymethyltransferase -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38445.61 Da        Isoelectric Point: 6.3879

>NTDB_id=477996 H9L06_RS05655 WP_281381725.1 1168486..1169538(-) (pilT) [Leucobacter denitrificans strain KACC 14055]
MGSPPIVRIDGDLTPIPGAHALLTVDALNDVIQGMLTGEQKRRFSEDLELDFAHSLAGVGRFRVNVYMQRQMIGAAFRRI
DNEVRDLESLGVPAHIRDLAFLPRGLVLVTGPTGSGKSTTLASVLDIINSERRCHIVTVEDPIEFMHQPKRSLIHQREVG
VDTRSFAVALKHVLRQDPDVILIGELRDPESISAALTAAETGHLVFATLHTVDAAQSIDRIVDAFPPHQQNQVRVQLSGS
LKAVVSQTLLRRKQGGRVVATEVLRMTSAIANLIREGETHQIYSVLQSGSENHMHTLDQSLLELVRTGEVEASEAREYAG
HARSFDDSVQSAAANGVNRQVAVTNNMWEY

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=477996 H9L06_RS05655 WP_281381725.1 1168486..1169538(-) (pilT) [Leucobacter denitrificans strain KACC 14055]
GTGGGTTCACCGCCGATTGTTCGTATTGATGGTGATCTGACACCAATACCGGGCGCGCATGCTCTGCTTACGGTTGACGC
CCTGAACGATGTGATTCAAGGCATGCTGACGGGCGAGCAGAAGCGTAGATTCAGCGAAGACCTTGAGCTTGACTTCGCTC
ACTCACTCGCCGGGGTGGGTCGGTTTCGAGTAAACGTGTACATGCAGCGACAAATGATCGGTGCAGCGTTTCGTCGTATC
GACAATGAAGTGCGCGATCTTGAGTCGCTCGGTGTGCCCGCCCATATTCGTGACCTCGCCTTCCTTCCGCGGGGTCTTGT
GCTCGTTACGGGACCCACGGGCTCTGGAAAATCCACGACCCTCGCCTCGGTTCTCGACATCATAAATTCTGAGCGTCGCT
GCCACATCGTGACAGTCGAGGACCCGATTGAGTTTATGCATCAACCGAAGCGAAGCCTCATTCATCAACGTGAAGTTGGT
GTTGACACGCGCAGCTTTGCGGTGGCCTTAAAACATGTACTCCGGCAGGATCCTGATGTGATTCTTATCGGCGAGCTTCG
CGATCCCGAGAGCATCTCGGCGGCACTGACGGCTGCGGAGACGGGCCATCTTGTGTTCGCCACTCTGCACACCGTTGATG
CGGCGCAGTCGATCGACCGAATTGTTGACGCGTTTCCACCACACCAGCAGAATCAGGTGCGCGTGCAGCTCTCAGGTAGC
TTGAAGGCCGTCGTTTCTCAGACGCTACTTCGTAGGAAGCAGGGCGGTCGAGTGGTTGCGACCGAAGTGCTCCGCATGAC
GTCTGCTATTGCGAATCTCATTCGAGAGGGTGAGACGCACCAGATTTACTCGGTTCTGCAGTCGGGGTCTGAGAATCATA
TGCATACGCTTGATCAGTCGCTACTCGAACTCGTGCGCACGGGTGAGGTTGAGGCCTCTGAGGCGCGTGAATACGCTGGA
CACGCACGTTCCTTCGATGACTCGGTGCAATCCGCGGCGGCGAATGGTGTGAATCGGCAGGTTGCCGTGACGAATAATAT
GTGGGAGTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(102-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

51.84

93.143

0.483
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.149

95.714

0.48
  pilT Acinetobacter baumannii strain A118

50.926

92.571

0.471
  pilT Acinetobacter nosocomialis M2

50.926

92.571

0.471
  pilT Acinetobacter baumannii D1279779

50.926

92.571

0.471
  pilT Pseudomonas stutzeri DSM 10701

49.849

94.571

0.471
  pilT Pseudomonas aeruginosa PAK

49.547

94.571

0.469
  pilT Legionella pneumophila strain ERS1305867

50.153

93.429

0.469
  pilT Legionella pneumophila strain Lp02

50.153

93.429

0.469
  pilT Neisseria gonorrhoeae MS11

48.615

92.857

0.451
  pilT Neisseria meningitidis 8013

48.615

92.857

0.451
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.524

90

0.446
  pilT Vibrio cholerae strain A1552

49.524

90

0.446
  pilU Vibrio cholerae strain A1552

43.987

90.286

0.397
  pilU Pseudomonas stutzeri DSM 10701

42.633

91.143

0.389
  pilU Acinetobacter baylyi ADP1

40.625

91.429

0.371