Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H9Q77_RS09585 Genome accession   NZ_CP060633
Coordinates   2075781..2076833 (-) Length   350 a.a.
NCBI ID   WP_118546095.1    Uniprot ID   -
Organism   Simiaoa sunii strain NSJ-8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2070781..2081833
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9Q77_RS09570 (H9Q77_09585) ispG 2071716..2072783 (-) 1068 WP_118550910.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  H9Q77_RS09575 (H9Q77_09590) dprA 2072845..2073993 (-) 1149 WP_249325326.1 DNA-processing protein DprA -
  H9Q77_RS09580 (H9Q77_09595) - 2074009..2075553 (-) 1545 WP_118550908.1 YifB family Mg chelatase-like AAA ATPase -
  H9Q77_RS09585 (H9Q77_09600) pilT 2075781..2076833 (-) 1053 WP_118546095.1 type IV pilus twitching motility protein PilT Machinery gene
  H9Q77_RS09590 (H9Q77_09605) - 2076850..2077923 (-) 1074 WP_118546093.1 HD domain-containing protein -
  H9Q77_RS09595 (H9Q77_09610) sigK 2078004..2078639 (-) 636 WP_022154131.1 RNA polymerase sporulation sigma factor SigK -
  H9Q77_RS09600 (H9Q77_09615) hemW 2078718..2079872 (-) 1155 WP_118546091.1 radical SAM family heme chaperone HemW -
  H9Q77_RS09605 (H9Q77_09620) lepA 2079896..2081713 (-) 1818 WP_022154133.1 translation elongation factor 4 -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38990.60 Da        Isoelectric Point: 6.2344

>NTDB_id=476854 H9Q77_RS09585 WP_118546095.1 2075781..2076833(-) (pilT) [Simiaoa sunii strain NSJ-8]
MPTIEEILRAAKEAGASDVHLTVGIPPKMRVNGNLITMDYPKMLPADTLEVLVNIMTEVQRERFEERGEYDMSFSIPNCG
RYRVNAYKQRGSVALAFRLVGTKVPSPEELGVPESVIDLYQRKRGLVLVTGPTGSGKSTTLAAIIDKINNNRDAHVITLE
DPIEYLHQHKMSMVNQREIGIDSNNYANALRAALREDPDVILVGEMRDFETISVAITAAETGHLVLSTLHTIGAASTVDR
VIDVFPPHQQQQIRVQLSNTLEAVISQQLIPTADGKHRVAAFEVMHANHAVRNLIREGKSHQLASVMQTNRKLGMITMDE
AIVQLYFEGKIDREHAIQFAQDPDNMENKI

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=476854 H9Q77_RS09585 WP_118546095.1 2075781..2076833(-) (pilT) [Simiaoa sunii strain NSJ-8]
ATGCCGACAATCGAAGAAATACTCAGAGCTGCCAAAGAAGCCGGAGCAAGTGATGTGCATCTTACGGTAGGTATTCCTCC
CAAAATGCGGGTGAACGGTAATCTGATCACGATGGATTATCCCAAAATGCTGCCTGCGGATACCCTGGAGGTGCTGGTCA
ACATCATGACAGAAGTGCAGAGGGAGCGTTTTGAGGAACGGGGAGAATATGATATGTCGTTCTCTATTCCGAACTGTGGA
CGTTATCGTGTCAATGCGTATAAACAAAGGGGATCTGTTGCCCTGGCATTCCGTCTGGTGGGAACCAAGGTACCTTCACC
GGAGGAACTGGGAGTACCGGAATCGGTGATTGATCTGTATCAGCGCAAACGTGGACTGGTGTTGGTCACCGGTCCTACCG
GAAGCGGTAAATCTACCACACTTGCTGCCATTATAGATAAGATCAATAATAACAGGGATGCTCACGTGATTACCCTGGAG
GACCCCATCGAGTACCTGCACCAGCATAAAATGTCCATGGTCAATCAGCGTGAGATCGGTATTGATTCCAACAATTATGC
CAATGCCTTAAGGGCAGCTCTCCGTGAAGATCCGGATGTTATCCTCGTGGGAGAAATGCGTGATTTTGAGACGATCAGTG
TAGCGATCACCGCGGCGGAGACGGGACATCTGGTATTGTCAACATTGCATACCATCGGTGCAGCCAGCACGGTAGACCGT
GTCATTGATGTATTCCCGCCGCACCAGCAGCAACAGATCCGGGTACAGCTGTCCAATACACTGGAAGCCGTTATTTCCCA
GCAGCTGATACCTACGGCAGACGGAAAACATCGTGTAGCGGCGTTTGAGGTCATGCATGCGAACCATGCAGTACGCAATC
TGATCCGTGAGGGAAAGTCCCATCAGCTGGCAAGTGTCATGCAGACCAACCGCAAGCTGGGGATGATCACCATGGACGAG
GCAATTGTCCAATTGTATTTTGAAGGCAAGATCGACAGAGAGCATGCCATCCAGTTCGCACAGGATCCGGACAACATGGA
AAATAAGATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.765

97.143

0.503
  pilT Neisseria meningitidis 8013

49.854

98

0.489
  pilT Neisseria gonorrhoeae MS11

49.563

98

0.486
  pilT Pseudomonas aeruginosa PAK

49.271

98

0.483
  pilT Pseudomonas stutzeri DSM 10701

48.98

98

0.48
  pilT Acinetobacter baylyi ADP1

48.688

98

0.477
  pilT Acinetobacter baumannii D1279779

48.688

98

0.477
  pilT Acinetobacter baumannii strain A118

48.688

98

0.477
  pilT Acinetobacter nosocomialis M2

48.688

98

0.477
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.024

94

0.451
  pilT Vibrio cholerae strain A1552

48.024

94

0.451
  pilT Legionella pneumophila strain Lp02

47.447

95.143

0.451
  pilT Legionella pneumophila strain ERS1305867

47.447

95.143

0.451
  pilU Pseudomonas stutzeri DSM 10701

42.566

98

0.417
  pilU Acinetobacter baylyi ADP1

39.255

99.714

0.391
  pilU Vibrio cholerae strain A1552

39.577

94.571

0.374