Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H9L11_RS04210 Genome accession   NZ_CP060589
Coordinates   904191..905231 (-) Length   346 a.a.
NCBI ID   WP_205391003.1    Uniprot ID   -
Organism   Vibrio sp. sp1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 899191..910231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9L11_RS04180 (H9L11_04180) endA 899336..900031 (+) 696 WP_025768831.1 deoxyribonuclease I -
  H9L11_RS04185 (H9L11_04185) rsmE 900145..900876 (+) 732 WP_025768830.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  H9L11_RS04190 (H9L11_04190) gshB 900890..901840 (+) 951 WP_005381021.1 glutathione synthase -
  H9L11_RS04195 (H9L11_04195) - 901968..902531 (+) 564 WP_005381020.1 YqgE/AlgH family protein -
  H9L11_RS04200 (H9L11_04200) ruvX 902567..902992 (+) 426 WP_005381017.1 Holliday junction resolvase RuvX -
  H9L11_RS04205 (H9L11_04205) pilU 903054..904160 (-) 1107 WP_005381016.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H9L11_RS04210 (H9L11_04210) pilT 904191..905231 (-) 1041 WP_205391003.1 type IV pilus twitching motility protein PilT Machinery gene
  H9L11_RS04215 (H9L11_04215) - 905260..905961 (+) 702 WP_005384521.1 YggS family pyridoxal phosphate-dependent enzyme -
  H9L11_RS04220 (H9L11_04220) proC 906121..906939 (+) 819 WP_205391004.1 pyrroline-5-carboxylate reductase -
  H9L11_RS04225 (H9L11_04225) - 906992..907549 (+) 558 WP_005381012.1 YggT family protein -
  H9L11_RS04230 (H9L11_04230) yggU 907549..907839 (+) 291 WP_005381011.1 DUF167 family protein YggU -
  H9L11_RS04235 (H9L11_04235) - 907968..908399 (+) 432 WP_005381010.1 DUF4426 domain-containing protein -
  H9L11_RS04240 (H9L11_04240) - 908518..909120 (+) 603 WP_025768828.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38464.95 Da        Isoelectric Point: 6.3875

>NTDB_id=476759 H9L11_RS04210 WP_205391003.1 904191..905231(-) (pilT) [Vibrio sp. sp1]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFKKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINCNHNKHVLTIED
PIEFVHNNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSVIQTGAAHGMQTMEQNA
RQLMAQGMVAREEVDSKIGLEVQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=476759 H9L11_RS04210 WP_205391003.1 904191..905231(-) (pilT) [Vibrio sp. sp1]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCCTCGGATCTACATCTTTCTGCAGGTGTCCCACCAAT
GGTTCGTATTGATGGGGATGTGAGAAAACTAGGCATTCCAGCGTTTACACACCAAGAAGTGCATCGTTTGGTTTTCGAAA
TCATGAACGATGCCCAACGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTCTCTTTTGAGTTGCATAACGTTGGCCGT
TTCCGTGTCAACGCTTTCAACCAATCTCGTGGTTGTGCGGCAGTATTCCGTACCATTCCAAGTAGCATTCCAACTCTGGA
AGAGCTAGAAGCGCCAGAGATCTTCAAAAAAATTGCGAACGCTGAAAAAGGCTTAGTTCTGGTCACTGGCCCAACAGGTT
CAGGTAAATCGACGACCTTAGCAGCCATTGTTGACTACATTAACTGTAATCACAATAAGCACGTACTCACAATTGAAGAT
CCGATTGAATTTGTTCACAACAACAATAAATGTTTGATCAACCAGCGTGAAGTTCACCGTGACACCCATAGTTTCCAAAA
CGCACTGCGCAGCGCATTACGTGAAGACCCGGATGTCATTCTGGTTGGTGAGATGCGTGACAAAGAAACCATCAGCCTTG
CGTTAACGGCGGCGGAAACGGGGCACTTAGTGTTCGGCACGTTACATACCAGCTCGGCGGCAAAAACCATTGACCGTATT
ATCGATGTGTTTCCTGGTAGTGATAAAGATATGGTTCGCTCGATGCTATCTGAGTCCCTTCGTTCGGTTATCGCGCAAAA
GCTTCTGAAGCGTAATGGTGGTGGCCGTATTGCATGCCATGAAATTATGATGGCAACGCCTGCGATTCGCAACTTGATCC
GCGAAGACAAAGTCGCGCAGATGTATTCGGTCATTCAAACAGGTGCGGCTCACGGCATGCAAACCATGGAGCAAAATGCT
CGTCAGTTAATGGCGCAAGGCATGGTTGCTCGTGAAGAGGTCGATAGTAAGATCGGATTAGAAGTGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.667

99.711

0.864
  pilT Vibrio cholerae strain A1552

86.667

99.711

0.864
  pilT Acinetobacter baumannii D1279779

72.121

95.376

0.688
  pilT Acinetobacter baumannii strain A118

72.121

95.376

0.688
  pilT Acinetobacter nosocomialis M2

71.818

95.376

0.685
  pilT Acinetobacter baylyi ADP1

71.429

95.087

0.679
  pilT Pseudomonas aeruginosa PAK

69.139

97.399

0.673
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.399

0.671
  pilT Legionella pneumophila strain Lp02

67.674

95.665

0.647
  pilT Legionella pneumophila strain ERS1305867

67.674

95.665

0.647
  pilT Neisseria meningitidis 8013

66.066

96.243

0.636
  pilT Neisseria gonorrhoeae MS11

65.766

96.243

0.633
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.145

91.908

0.488
  pilU Vibrio cholerae strain A1552

41.317

96.532

0.399
  pilU Pseudomonas stutzeri DSM 10701

40.719

96.532

0.393
  pilU Acinetobacter baylyi ADP1

39.062

92.486

0.361