Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H3W50_RS22245 Genome accession   NZ_CP060441
Coordinates   4501073..4502053 (+) Length   326 a.a.
NCBI ID   WP_038633604.1    Uniprot ID   A0AAU7IRA2
Organism   Citrobacter cronae strain S175-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4496073..4507053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3W50_RS22215 ansB 4496586..4497632 (+) 1047 WP_038633619.1 L-asparaginase 2 -
  H3W50_RS22220 hemW 4497749..4498885 (-) 1137 WP_038633617.1 radical SAM family heme chaperone HemW -
  H3W50_RS22225 - 4498878..4499471 (-) 594 WP_038633614.1 XTP/dITP diphosphatase -
  H3W50_RS22230 yggU 4499479..4499769 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  H3W50_RS22235 - 4499766..4500332 (-) 567 WP_038633609.1 YggT family protein -
  H3W50_RS22240 - 4500351..4501055 (-) 705 WP_038633606.1 YggS family pyridoxal phosphate-dependent enzyme -
  H3W50_RS22245 pilT 4501073..4502053 (+) 981 WP_038633604.1 type IV pilus twitching motility protein PilT Machinery gene
  H3W50_RS22250 ruvX 4502050..4502466 (-) 417 WP_038633601.1 Holliday junction resolvase RuvX -
  H3W50_RS22255 - 4502466..4503029 (-) 564 WP_032940453.1 YqgE/AlgH family protein -
  H3W50_RS22260 gshB 4503141..4504088 (-) 948 WP_038633598.1 glutathione synthase -
  H3W50_RS22265 rsmE 4504101..4504832 (-) 732 WP_038633593.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  H3W50_RS22270 endA 4504907..4505614 (-) 708 WP_038633590.1 deoxyribonuclease I -
  H3W50_RS22275 - 4505709..4506206 (-) 498 WP_038633587.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35910.20 Da        Isoelectric Point: 6.7577

>NTDB_id=476196 H3W50_RS22245 WP_038633604.1 4501073..4502053(+) (pilT) [Citrobacter cronae strain S175-1]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGNVEIAPFTTPDVETLLMCWLSEQQQVQWQKQGQIDFAITLTNSRRLRA
SAFVHQQGISLALRLLPLDCPCLDDLQTPAALPELLQSENGLILVTGATGSGKSTTLAAMVSYLNQHVAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAPEKDPVRNQLADSLRAVLSQKLEQDKQGGRVALFELLINTPAVGNLIREGKTHQLPGIMQTGQQTGMQTFTQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=476196 H3W50_RS22245 WP_038633604.1 4501073..4502053(+) (pilT) [Citrobacter cronae strain S175-1]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAATGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
TTGGCGCATACGCGGAAATGTCGAAATCGCGCCGTTTACCACGCCTGACGTAGAGACGTTGCTGATGTGCTGGCTCAGCG
AGCAACAACAGGTACAGTGGCAGAAACAGGGACAGATAGATTTTGCCATTACGCTGACGAACTCACGGCGTCTGCGTGCG
AGCGCATTTGTGCATCAGCAGGGGATCTCTCTGGCGCTAAGGCTATTGCCGCTTGATTGCCCATGTTTGGATGATCTCCA
GACTCCCGCCGCCTTACCTGAGCTGCTGCAAAGTGAAAATGGATTAATTCTGGTGACCGGCGCTACCGGCAGCGGTAAAT
CAACGACCCTGGCGGCGATGGTGTCATATCTTAATCAGCACGTCGCGGGACATATTTTGACGCTGGAAGATCCCATTGAA
TATCGTTACACCAGCCAGCGTTGTCTCATTCAACAACGGGAGGTAGGCGTACACTGCGCTTCTTTCGCCGCCGGTTTGCG
CGGCGCGCTGCGCGAAGATCCTGATGTCATTCTGCTGGGCGAGCTGCGCGACGTGGAAACCATTCGTCTGGCGTTAACGG
CTGCGGAAACCGGGCATCTGGTGCTGGCAACGTTACACACGCGAGGGGCGGCTCAGGCTATTGCGCGGCTGGTAGATACC
TTTCCTGCACCGGAGAAAGATCCGGTGCGTAATCAGCTGGCAGACAGCCTGCGGGCAGTGCTTTCGCAAAAGCTGGAACA
AGATAAGCAGGGAGGACGCGTGGCGCTATTCGAACTGCTCATCAACACGCCCGCCGTTGGTAACCTGATCCGTGAAGGGA
AAACGCATCAGCTACCCGGCATAATGCAAACCGGGCAACAGACAGGCATGCAGACCTTTACGCAAAGCCTGCAGCAGCGC
CAGGCGCAGGGACGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(72-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

45.652

98.773

0.451
  pilT Legionella pneumophila strain Lp02

45.652

98.773

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.81

98.466

0.402
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Acinetobacter baylyi ADP1

39.077

99.693

0.39
  pilU Vibrio cholerae strain A1552

40.323

95.092

0.383