Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H3V66_RS00415 Genome accession   NZ_CP060440
Coordinates   86964..87986 (+) Length   340 a.a.
NCBI ID   WP_240023515.1    Uniprot ID   -
Organism   Serratia marcescens strain M158-1-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 81964..92986
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3V66_RS00375 - 82278..82508 (+) 231 WP_240023512.1 DUF4160 domain-containing protein -
  H3V66_RS00380 - 82492..82740 (+) 249 WP_038879292.1 DUF2442 domain-containing protein -
  H3V66_RS00385 hemW 82776..83918 (-) 1143 WP_240023513.1 radical SAM family heme chaperone HemW -
  H3V66_RS00390 - 83911..84504 (-) 594 WP_038879287.1 XTP/dITP diphosphatase -
  H3V66_RS00395 yggU 84522..84812 (-) 291 WP_033649585.1 DUF167 family protein YggU -
  H3V66_RS00400 - 84809..85363 (-) 555 WP_033635944.1 YggT family protein -
  H3V66_RS00405 proC 85395..86216 (-) 822 WP_126180722.1 pyrroline-5-carboxylate reductase -
  H3V66_RS00410 - 86236..86943 (-) 708 WP_240023514.1 YggS family pyridoxal phosphate-dependent enzyme -
  H3V66_RS00415 pilT 86964..87986 (+) 1023 WP_240023515.1 type IV pilus twitching motility protein PilT Machinery gene
  H3V66_RS00420 ruvX 87983..88405 (-) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  H3V66_RS00425 - 88405..88968 (-) 564 WP_033635940.1 YqgE/AlgH family protein -
  H3V66_RS00430 gshB 89114..90064 (-) 951 WP_038879277.1 glutathione synthase -
  H3V66_RS00435 rsmE 90075..90806 (-) 732 WP_174136631.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  H3V66_RS00440 endA 90876..91571 (-) 696 WP_038879275.1 deoxyribonuclease I -
  H3V66_RS00445 - 91673..92185 (-) 513 WP_225527964.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36446.81 Da        Isoelectric Point: 6.8799

>NTDB_id=476157 H3V66_RS00415 WP_240023515.1 86964..87986(+) (pilT) [Serratia marcescens strain M158-1-1]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQIDLALHRPGGE
RLRANVFQQCAGMSLALRRIAGQVPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDVATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCVGEGAGD

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=476157 H3V66_RS00415 WP_240023515.1 86964..87986(+) (pilT) [Serratia marcescens strain M158-1-1]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGCCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGATCGATCTGGCGCTGCACAGGCCGGGCGGGGAG
CGGTTGCGGGCTAACGTTTTTCAGCAATGCGCGGGGATGTCTCTCGCATTGCGGCGCATCGCCGGGCAGGTGCCTTCGCT
TGCCGAGCTGGCGGCCCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGACGACGGGCTTCTTCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACATTTTAACGCTGGAA
GATCCGATCGAATTCCTGCACCGCAGCCGGCGATCGCTGATTCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATGTCGCCACCATTCGGT
TGGCGCTCACTGCGGCTGAGACCGGCCATCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTGGAAAGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGTTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGCGGCGGCCCGGCGGCGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGATTGTGTTGGCGAAGGGGCGGGGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

51.662

97.353

0.503
  pilT Neisseria meningitidis 8013

50.298

98.824

0.497
  pilT Acinetobacter nosocomialis M2

51.212

97.059

0.497
  pilT Acinetobacter baumannii D1279779

51.212

97.059

0.497
  pilT Acinetobacter baumannii strain A118

51.212

97.059

0.497
  pilT Neisseria gonorrhoeae MS11

50.453

97.353

0.491
  pilT Pseudomonas stutzeri DSM 10701

48.968

99.706

0.488
  pilT Pseudomonas aeruginosa PAK

50

97.059

0.485
  pilT Legionella pneumophila strain ERS1305867

49.245

97.353

0.479
  pilT Legionella pneumophila strain Lp02

49.245

97.353

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.322

100

0.456
  pilU Vibrio cholerae strain A1552

40.123

95.294

0.382
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.889

95.294

0.371