Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H8F27_RS07305 Genome accession   NZ_CP060395
Coordinates   1371400..1372479 (-) Length   359 a.a.
NCBI ID   WP_197152697.1    Uniprot ID   A0A7T1ICC0
Organism   Synechococcus sp. CBW1108     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1366400..1377479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H8F27_RS07285 (H8F27_07275) - 1367239..1367766 (+) 528 WP_197152689.1 RNA-binding protein -
  H8F27_RS07290 (H8F27_07280) dusA 1367795..1368820 (-) 1026 WP_197152691.1 tRNA dihydrouridine(20/20a) synthase DusA -
  H8F27_RS07295 (H8F27_07285) - 1368856..1370121 (+) 1266 WP_197152693.1 aminoacetone oxidase family FAD-binding enzyme -
  H8F27_RS07300 (H8F27_07290) - 1370137..1371396 (-) 1260 WP_197152695.1 type II secretion system F family protein -
  H8F27_RS07305 (H8F27_07295) pilT 1371400..1372479 (-) 1080 WP_197152697.1 type IV pilus twitching motility protein PilT Machinery gene
  H8F27_RS07310 (H8F27_07300) pilB/pilB1 1372509..1374008 (-) 1500 WP_197152698.1 GspE/PulE family protein Machinery gene
  H8F27_RS07315 (H8F27_07305) grpE 1374097..1374843 (+) 747 WP_370594479.1 nucleotide exchange factor GrpE -
  H8F27_RS07320 (H8F27_07310) dnaJ 1374843..1375961 (+) 1119 WP_197152700.1 molecular chaperone DnaJ -
  H8F27_RS07325 (H8F27_07315) - 1375958..1376218 (+) 261 WP_197152702.1 sulfurtransferase TusA family protein -
  H8F27_RS07330 (H8F27_07320) rsgA 1376208..1377332 (+) 1125 WP_197152709.1 ribosome small subunit-dependent GTPase A -

Sequence

Protein


Download         Length: 359 a.a.        Molecular weight: 39378.19 Da        Isoelectric Point: 6.9057

>NTDB_id=475678 H8F27_RS07305 WP_197152697.1 1371400..1372479(-) (pilT) [Synechococcus sp. CBW1108]
MAVMIEELMGQLVADGGSDLHISAGLPPYGRFNGQLRPMQPEILEEEACNRLIFSLLNNSQRKQLEQTWELDCAYGLKGL
ARFRVNVYRQRGSYAACLRALANKVPSLESLGLPPIVEEMSHTPKGLILVTGPTGSGKTTTLAAILEHINQTRAEHILTI
EDPIEFTYKPAKSIIHQRQVNDDTRSFSNALRAALREDPDVILVGELRDLETIQLAITAAETGHLVFATLHTSSAAQTVD
RMVDVFPAGQQTQIRVQLSTSLVAIFAQTLCKRKHQGPGENGRVLAQEIMVNTPATANLIREAKTAQLYSQIQTGGQLSM
QTLEKALANLVLSNQISLDTALLKSSRPEELKRLVSQLG

Nucleotide


Download         Length: 1080 bp        

>NTDB_id=475678 H8F27_RS07305 WP_197152697.1 1371400..1372479(-) (pilT) [Synechococcus sp. CBW1108]
ATGGCCGTAATGATCGAAGAGCTGATGGGCCAACTGGTTGCAGATGGGGGCAGCGATCTGCACATCAGCGCCGGCCTGCC
GCCCTATGGAAGGTTCAATGGCCAACTCAGGCCCATGCAGCCTGAAATCCTCGAAGAAGAAGCCTGCAACAGGCTGATCT
TTTCGTTGCTGAACAACTCACAGCGAAAACAACTGGAACAAACCTGGGAGCTCGATTGTGCCTATGGCCTCAAAGGCCTA
GCCCGCTTCAGGGTCAACGTCTATCGGCAGCGGGGCAGCTATGCCGCCTGCCTGAGGGCCCTGGCCAATAAGGTGCCATC
GCTCGAGAGCCTGGGGCTGCCGCCGATCGTTGAGGAAATGAGCCACACGCCAAAGGGGCTGATCCTGGTAACAGGCCCCA
CAGGTTCGGGTAAAACCACCACCCTGGCCGCAATCCTGGAGCACATTAATCAAACACGAGCAGAGCATATTCTCACCATT
GAAGACCCGATTGAATTCACCTATAAGCCAGCTAAAAGTATCATCCACCAACGCCAGGTAAACGACGATACCCGCAGCTT
TTCAAATGCACTGAGGGCAGCCCTTCGCGAGGATCCAGATGTGATTTTGGTAGGGGAACTGCGCGACCTCGAAACCATCC
AGCTGGCCATCACAGCCGCCGAAACTGGTCACCTCGTCTTCGCAACCCTGCACACCAGCTCGGCCGCCCAAACCGTCGAC
CGGATGGTTGATGTGTTTCCAGCTGGCCAACAAACCCAGATCCGGGTGCAACTGAGCACCAGCCTGGTGGCGATCTTTGC
CCAAACCCTTTGCAAACGCAAGCACCAGGGCCCTGGCGAGAACGGCCGGGTACTGGCCCAGGAGATCATGGTCAACACCC
CGGCAACGGCAAACCTGATCAGGGAAGCAAAAACGGCCCAGCTTTATTCCCAGATCCAAACAGGGGGACAACTGTCCATG
CAAACCCTGGAAAAGGCCCTTGCCAACCTGGTACTAAGTAACCAGATCAGCCTGGATACGGCCCTTTTAAAATCATCAAG
ACCTGAAGAACTCAAACGGCTTGTGAGCCAATTGGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7T1ICC0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

54.006

93.872

0.507
  pilT Legionella pneumophila strain ERS1305867

54.006

93.872

0.507
  pilT Pseudomonas aeruginosa PAK

52.161

96.657

0.504
  pilT Pseudomonas stutzeri DSM 10701

51.297

96.657

0.496
  pilT Acinetobacter baylyi ADP1

50.432

96.657

0.487
  pilT Acinetobacter baumannii D1279779

50.432

96.657

0.487
  pilT Acinetobacter baumannii strain A118

50.432

96.657

0.487
  pilT Acinetobacter nosocomialis M2

50.432

96.657

0.487
  pilT Neisseria meningitidis 8013

49.138

96.936

0.476
  pilT Neisseria gonorrhoeae MS11

48.851

96.936

0.474
  pilT Vibrio cholerae strain A1552

51.682

91.086

0.471
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.682

91.086

0.471
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.563

96.936

0.471
  pilU Pseudomonas stutzeri DSM 10701

38.873

98.886

0.384
  pilU Acinetobacter baylyi ADP1

37.892

97.772

0.37