Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   H8F27_RS07310 Genome accession   NZ_CP060395
Coordinates   1372509..1374008 (-) Length   499 a.a.
NCBI ID   WP_197152698.1    Uniprot ID   A0A7T1MPC9
Organism   Synechococcus sp. CBW1108     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1367509..1379008
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H8F27_RS07290 (H8F27_07280) dusA 1367795..1368820 (-) 1026 WP_197152691.1 tRNA dihydrouridine(20/20a) synthase DusA -
  H8F27_RS07295 (H8F27_07285) - 1368856..1370121 (+) 1266 WP_197152693.1 aminoacetone oxidase family FAD-binding enzyme -
  H8F27_RS07300 (H8F27_07290) - 1370137..1371396 (-) 1260 WP_197152695.1 type II secretion system F family protein -
  H8F27_RS07305 (H8F27_07295) pilT 1371400..1372479 (-) 1080 WP_197152697.1 type IV pilus twitching motility protein PilT Machinery gene
  H8F27_RS07310 (H8F27_07300) pilB/pilB1 1372509..1374008 (-) 1500 WP_197152698.1 GspE/PulE family protein Machinery gene
  H8F27_RS07315 (H8F27_07305) grpE 1374097..1374843 (+) 747 WP_370594479.1 nucleotide exchange factor GrpE -
  H8F27_RS07320 (H8F27_07310) dnaJ 1374843..1375961 (+) 1119 WP_197152700.1 molecular chaperone DnaJ -
  H8F27_RS07325 (H8F27_07315) - 1375958..1376218 (+) 261 WP_197152702.1 sulfurtransferase TusA family protein -
  H8F27_RS07330 (H8F27_07320) rsgA 1376208..1377332 (+) 1125 WP_197152709.1 ribosome small subunit-dependent GTPase A -
  H8F27_RS07335 (H8F27_07325) - 1377307..1377648 (-) 342 WP_197152711.1 YbaB/EbfC family nucleoid-associated protein -
  H8F27_RS07340 (H8F27_07330) murB 1377684..1378595 (-) 912 WP_197152713.1 UDP-N-acetylmuramate dehydrogenase -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 54224.69 Da        Isoelectric Point: 6.1310

>NTDB_id=475679 H8F27_RS07310 WP_197152698.1 1372509..1374008(-) (pilB/pilB1) [Synechococcus sp. CBW1108]
MSSTPSRPGQMLPPSSPPAATRSLTQELGIASSGPGQLQEIAEIEDLETSAAAGASGTASPIISLVDRILIEALTSGASD
IHVEPQENTLEVRFRLDGVLQKQFEDLPKSLTPAVTSRIKIMAELDIAERRLPQDGRIRRMFRGRKMDFRVSTLPGRNGE
KICLRLLDSGATQLGLDTLITDGEARQTLREMGSKPYGMLLVTGPTGSGKSTTLYALLAERNDPGINISTVEDPIEYTLP
GITQTQVNRDKGFDFSTALRAFMRQDPDVLLVGETRDLETAKTAIEAALTGHLVMTTLHCNDAASAIARLSEMGVEPFMV
SASLLGIVSQRLVRRVCPACRISYEPSPEELARFGLMGSNETHVTFYKANTQSYKANTQSRNDHNRCSSCNGAGYKGRVG
VYEILRMSDGLAAAVAKHESTDVLRRMAIENGMKTLLGYGLQLVRDGHTTLGEVERMLLTDSGLESERRARSLSTLTCTS
CGAGLHDEWLECPYCLTTR

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=475679 H8F27_RS07310 WP_197152698.1 1372509..1374008(-) (pilB/pilB1) [Synechococcus sp. CBW1108]
ATGAGCTCCACACCGTCGCGTCCAGGCCAAATGCTGCCCCCCAGTTCCCCACCGGCTGCGACCCGATCCCTGACCCAGGA
GCTGGGGATAGCCAGCTCGGGCCCTGGCCAGCTTCAGGAGATTGCCGAGATTGAAGATCTGGAGACCAGCGCCGCCGCGG
GGGCCAGCGGCACGGCATCGCCAATCATCAGCCTGGTGGACCGAATCCTGATCGAGGCCCTCACCTCCGGGGCGAGTGAC
ATCCACGTTGAACCCCAGGAAAACACCCTGGAAGTGCGCTTCCGCCTCGACGGCGTGCTGCAAAAGCAGTTTGAAGATCT
GCCCAAAAGCCTCACTCCGGCGGTCACCTCCCGCATCAAGATCATGGCTGAGCTCGACATAGCCGAGCGTCGCCTGCCCC
AGGACGGCCGCATCCGGCGCATGTTCAGGGGTCGGAAAATGGATTTCCGCGTCAGCACCCTGCCGGGCCGCAACGGAGAG
AAGATCTGCCTGCGCCTGCTGGACAGCGGGGCCACCCAATTGGGCCTGGACACCCTGATCACCGATGGGGAGGCCCGCCA
GACCCTGCGGGAGATGGGATCCAAGCCTTACGGCATGCTGCTGGTAACCGGGCCAACTGGATCTGGCAAATCCACGACCC
TCTATGCACTGCTGGCCGAGCGCAACGATCCGGGGATAAATATCTCCACGGTTGAAGATCCAATCGAATACACCCTGCCT
GGCATCACCCAAACCCAGGTCAACCGTGATAAGGGATTTGACTTCAGTACGGCGCTGCGCGCCTTCATGCGTCAGGACCC
GGACGTGCTGCTCGTTGGCGAGACCCGCGACCTGGAAACGGCAAAAACAGCTATCGAGGCGGCCCTCACAGGCCACCTGG
TAATGACAACCCTGCACTGCAACGATGCGGCCAGCGCCATCGCACGCCTCAGTGAGATGGGCGTCGAACCCTTCATGGTG
AGTGCTTCCCTGCTCGGAATCGTCTCCCAGCGCCTGGTGCGCCGGGTATGCCCAGCCTGTCGAATCAGCTATGAACCCAG
CCCCGAAGAGCTGGCCAGATTTGGCTTAATGGGAAGCAACGAGACCCATGTCACCTTTTACAAAGCAAACACCCAAAGTT
ACAAAGCAAACACCCAAAGCCGCAACGATCACAACCGCTGCAGCAGCTGCAACGGCGCCGGCTACAAAGGCCGCGTCGGT
GTATACGAGATTCTGCGCATGAGCGACGGGCTTGCCGCTGCGGTGGCCAAACATGAAAGCACCGATGTGCTGAGGCGAAT
GGCCATAGAAAACGGCATGAAAACCCTGCTGGGCTATGGACTCCAGCTGGTGCGCGATGGCCACACAACCCTGGGCGAAG
TTGAGCGCATGTTGTTGACCGATTCCGGCCTCGAGTCAGAGCGACGGGCGCGATCATTGAGCACCCTTACCTGCACAAGT
TGCGGCGCTGGCCTGCACGATGAGTGGCTCGAATGCCCCTACTGTCTAACCACCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(69-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7T1MPC9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

63.516

91.182

0.579
  pilF Thermus thermophilus HB27

43.946

89.379

0.393
  pilB Vibrio campbellii strain DS40M4

44.318

88.176

0.391
  pilB Vibrio cholerae strain A1552

45.047

84.97

0.383
  pilB Vibrio parahaemolyticus RIMD 2210633

45

84.168

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.026

84.77

0.365