Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JR901_RS04170 Genome accession   NZ_CP070906
Coordinates   871166..872146 (+) Length   326 a.a.
NCBI ID   WP_021557194.1    Uniprot ID   -
Organism   Escherichia coli strain 88COLEC     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 866166..877146
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JR901_RS04140 (JR901_04140) yggM 866767..867774 (+) 1008 WP_000745257.1 DUF1202 family protein -
  JR901_RS04145 (JR901_04145) hemW 867843..868979 (-) 1137 WP_000239930.1 radical SAM family heme chaperone HemW -
  JR901_RS04150 (JR901_04150) rdgB 868972..869565 (-) 594 WP_001174746.1 XTP/dITP diphosphatase -
  JR901_RS04155 (JR901_04155) yggU 869573..869863 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  JR901_RS04160 (JR901_04160) yggT 869860..870426 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  JR901_RS04165 (JR901_04165) yggS 870444..871148 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  JR901_RS04170 (JR901_04170) pilT 871166..872146 (+) 981 WP_021557194.1 type IV pilus twitching motility protein PilT Machinery gene
  JR901_RS04175 (JR901_04175) ruvX 872337..872753 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  JR901_RS04180 (JR901_04180) yqgE 872753..873316 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  JR901_RS04185 (JR901_04185) gshB 873425..874375 (-) 951 WP_000593284.1 glutathione synthase -
  JR901_RS04190 (JR901_04190) rsmE 874388..875119 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  JR901_RS04195 (JR901_04195) endA 875199..875906 (-) 708 WP_000286497.1 deoxyribonuclease I -
  JR901_RS04200 (JR901_04200) yggI 876001..876498 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35842.05 Da        Isoelectric Point: 5.2304

>NTDB_id=474865 JR901_RS04170 WP_021557194.1 871166..872146(+) (pilT) [Escherichia coli strain 88COLEC]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSCCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLNCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=474865 JR901_RS04170 WP_021557194.1 871166..872146(+) (pilT) [Escherichia coli strain 88COLEC]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGTTGTTACCTTCGTGCTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGTAAAT
CCACCACGCTGGCGGCAATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATCGGTTTGAATTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGGCTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCAGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGA
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(63-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

37.346

99.387

0.371