Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HPRAE_RS04355 Genome accession   NC_017455
Coordinates   939337..940407 (+) Length   356 a.a.
NCBI ID   WP_280985076.1    Uniprot ID   -
Organism   Halanaerobium praevalens DSM 2228     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 934337..945407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPRAE_RS04330 (Hprae_0835) - 934597..934851 (-) 255 WP_014553022.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  HPRAE_RS04335 (Hprae_0836) - 935053..935484 (+) 432 WP_014553023.1 Fur family transcriptional regulator -
  HPRAE_RS04340 (Hprae_0837) - 935560..936252 (+) 693 WP_014553024.1 Crp/Fnr family transcriptional regulator -
  HPRAE_RS04345 (Hprae_0838) - 936380..937291 (+) 912 WP_014553025.1 shikimate dehydrogenase -
  HPRAE_RS04350 (Hprae_0839) - 937625..939322 (+) 1698 WP_014553026.1 GspE/PulE family protein -
  HPRAE_RS04355 (Hprae_0840) pilT 939337..940407 (+) 1071 WP_280985076.1 type IV pilus twitching motility protein PilT Machinery gene
  HPRAE_RS04360 (Hprae_0841) - 940437..941681 (+) 1245 WP_014553028.1 type II secretion system F family protein -
  HPRAE_RS04365 (Hprae_0842) - 941845..942300 (+) 456 WP_014553029.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HPRAE_RS04370 (Hprae_0843) - 942355..944097 (+) 1743 WP_014553030.1 O-antigen ligase family protein -
  HPRAE_RS11310 (Hprae_0844) - 944121..944555 (+) 435 WP_014553031.1 type II secretion system protein -
  HPRAE_RS04380 (Hprae_0845) - 944585..945328 (+) 744 WP_014553032.1 A24 family peptidase -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40147.31 Da        Isoelectric Point: 9.4809

>NTDB_id=47346 HPRAE_RS04355 WP_280985076.1 939337..940407(+) (pilT) [Halanaerobium praevalens DSM 2228]
MICLELKKLLEIVSQDDQISDLHLTVFSPPYIRRNGKLEPYSQYEGELSVADTENFAKAMMNKKQYQEFKAQGELDFSYR
MPGTSRFRVNAYHQRGSVGIALRIIPNQVPTIEAMNLPKVLKKLAYQRMGLVLSTGPTGSGKSTTQAAIINEINKNRQCH
IMTLEDPIEYLHKHNKSLVHQREVGSDTNNFKRGLRACLRQDPDVILVGEMRDLETIQIALEAAETGHLVLATLHTNSAP
ATIDRIIDVFPAAQQGQIRIQLASVISAVIAQQLIPTKDQSGRVAAFEIMMATSAIRNIIREGKTNQIYSAIQTGGRYQM
ITMDNSLLKLYKRDKITKESVLHHCNDIKYVNEKLR

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=47346 HPRAE_RS04355 WP_280985076.1 939337..940407(+) (pilT) [Halanaerobium praevalens DSM 2228]
GTGATCTGTTTGGAATTAAAAAAATTATTAGAAATAGTATCTCAGGATGATCAAATATCTGATCTTCATTTAACAGTTTT
TTCACCTCCTTATATTCGCAGAAATGGGAAATTAGAACCATATTCACAATATGAGGGAGAATTAAGTGTTGCAGATACTG
AAAATTTTGCTAAAGCAATGATGAATAAAAAACAATACCAGGAGTTTAAAGCTCAAGGAGAATTAGATTTTTCTTATCGA
ATGCCAGGCACCTCACGTTTTCGAGTTAATGCTTATCATCAAAGAGGAAGTGTTGGAATTGCTTTAAGAATTATTCCAAA
TCAAGTACCAACAATTGAAGCCATGAACTTGCCTAAAGTTTTAAAAAAACTAGCTTACCAAAGAATGGGTTTAGTTTTAA
GTACTGGCCCAACAGGTAGTGGTAAATCTACAACTCAGGCAGCAATTATTAATGAAATAAATAAAAATAGACAGTGTCAT
ATTATGACTTTAGAAGATCCAATTGAATATCTGCATAAACATAATAAGAGTCTTGTTCATCAGCGTGAAGTAGGTAGTGA
TACTAATAATTTCAAAAGAGGTTTAAGAGCCTGTTTACGCCAAGATCCAGATGTAATTTTAGTTGGAGAAATGAGAGATT
TGGAGACTATTCAAATTGCTTTAGAGGCGGCAGAGACGGGTCATTTAGTTTTAGCAACTCTGCATACTAATAGTGCTCCT
GCTACTATTGATCGAATAATTGATGTTTTCCCAGCAGCTCAACAAGGACAGATTAGGATTCAATTAGCTTCAGTTATTAG
TGCAGTTATAGCTCAACAGTTAATTCCAACTAAAGATCAGAGTGGAAGAGTAGCAGCTTTTGAAATTATGATGGCAACTT
CTGCAATTAGAAATATAATTAGAGAAGGGAAGACAAATCAGATTTATTCTGCTATTCAAACTGGTGGTCGTTATCAAATG
ATTACAATGGATAATTCTCTTTTGAAATTGTACAAGAGAGATAAAATTACTAAAGAATCAGTATTACATCACTGTAATGA
TATAAAATATGTTAATGAAAAATTGAGATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(16-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.929

94.663

0.492
  pilT Acinetobacter baylyi ADP1

48.649

93.539

0.455
  pilT Legionella pneumophila strain ERS1305867

48.071

94.663

0.455
  pilT Legionella pneumophila strain Lp02

48.071

94.663

0.455
  pilT Pseudomonas aeruginosa PAK

49.693

91.573

0.455
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.633

94.944

0.452
  pilT Vibrio cholerae strain A1552

47.633

94.944

0.452
  pilT Pseudomonas stutzeri DSM 10701

49.383

91.011

0.449
  pilT Acinetobacter nosocomialis M2

49.844

90.169

0.449
  pilT Acinetobacter baumannii strain A118

49.844

90.169

0.449
  pilT Acinetobacter baumannii D1279779

49.844

90.169

0.449
  pilT Neisseria meningitidis 8013

47.531

91.011

0.433
  pilT Neisseria gonorrhoeae MS11

46.605

91.011

0.424
  pilU Pseudomonas stutzeri DSM 10701

42.45

98.596

0.419
  pilU Vibrio cholerae strain A1552

41.018

93.82

0.385
  pilU Acinetobacter baylyi ADP1

37.861

97.191

0.368

Multiple sequence alignment