Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H5A40_RS01860 Genome accession   NZ_CP059959
Coordinates   406317..407342 (-) Length   341 a.a.
NCBI ID   WP_205591857.1    Uniprot ID   -
Organism   Pectobacterium brasiliense strain IPO:4062 NAK:237     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 401317..412342
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5A40_RS01845 (H5A40_01845) nsrR 401649..402074 (-) 426 WP_014916532.1 nitric oxide-sensing transcriptional repressor NsrR -
  H5A40_RS01850 (H5A40_01850) - 402418..403989 (+) 1572 WP_205591855.1 methyl-accepting chemotaxis protein -
  H5A40_RS01855 (H5A40_01855) - 404516..406348 (+) 1833 WP_205591856.1 sensor domain-containing diguanylate cyclase -
  H5A40_RS01860 (H5A40_01860) pilT 406317..407342 (-) 1026 WP_205591857.1 type IV pilus twitching motility protein PilT Machinery gene
  H5A40_RS01865 (H5A40_01865) - 407363..408076 (+) 714 WP_205546957.1 YggS family pyridoxal phosphate-dependent enzyme -
  H5A40_RS01870 (H5A40_01870) proC 408171..408992 (+) 822 WP_205592397.1 pyrroline-5-carboxylate reductase -
  H5A40_RS01875 (H5A40_01875) - 409041..409595 (+) 555 WP_010295003.1 YggT family protein -
  H5A40_RS01880 (H5A40_01880) yggU 409592..409882 (+) 291 WP_010279110.1 DUF167 family protein YggU -
  H5A40_RS01885 (H5A40_01885) - 409900..410493 (+) 594 WP_014916538.1 XTP/dITP diphosphatase -
  H5A40_RS01890 (H5A40_01890) hemW 410486..411628 (+) 1143 WP_205591858.1 radical SAM family heme chaperone HemW -
  H5A40_RS01895 (H5A40_01895) - 411857..412117 (+) 261 WP_039558289.1 type II toxin-antitoxin system ParD family antitoxin -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 36833.01 Da        Isoelectric Point: 6.4949

>NTDB_id=472634 H5A40_RS01860 WP_205591857.1 406317..407342(-) (pilT) [Pectobacterium brasiliense strain IPO:4062 NAK:237]
MELDEWMARSVKHNASDLHLCSGHPPVLPVDGRLQPENTLPRLTSDQVAQWCDAWLEPAQREQLRQAGQAGQVDGALMLP
DGQRLRVNVFRQREGLSAALRIIPSAQPSLAALQAPPVLSTLLEKPNGLILVTGATGSGKSTTLAAMLGALNDSSDRHVI
TLEDPIEFIHASRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQA
VDRLIDVFPGEEKAYVRSQLATCLQAVVTQRLLPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTF
EQSYQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=472634 H5A40_RS01860 WP_205591857.1 406317..407342(-) (pilT) [Pectobacterium brasiliense strain IPO:4062 NAK:237]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCGGT
GCTGCCTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGTCGGATCAGGTGGCACAATGGTGCGATG
CCTGGCTCGAACCCGCTCAGAGAGAGCAACTGCGGCAGGCTGGGCAGGCTGGGCAGGTGGACGGGGCATTAATGCTGCCC
GATGGACAACGCCTGCGGGTGAATGTGTTTCGTCAGCGGGAAGGGCTGTCTGCGGCGCTACGTATTATCCCGTCCGCTCA
GCCTTCATTGGCGGCGCTACAGGCACCACCCGTTCTCTCGACGCTGCTGGAGAAACCGAACGGACTGATCCTAGTTACCG
GTGCAACGGGGAGCGGTAAATCTACGACGCTGGCGGCCATGCTTGGGGCGTTAAACGACAGCAGCGATCGCCATGTGATT
ACGCTGGAGGATCCGATCGAGTTTATCCATGCCAGCCGACGTTGCCTGATTCAACAGAGGGAGATTGGTGCACACAGTGC
GTCCTTTGCGCAGGCGCTACGGGCCGCATTGCGGGAGGATCCCGATGTCATTCTGCTGGGCGAATTGCGCGATACGGAGA
CGATTCGGCTGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGTTGTCGACGCTGCATACACGTAGTGCATCGCAGGCG
GTCGATCGTCTGATTGACGTCTTTCCCGGTGAAGAGAAAGCCTATGTTCGCAGCCAGTTAGCTACCTGCTTGCAGGCCGT
GGTGACACAAAGGCTGTTGCCCGCTGCGCAAGGTGGCCGAATTGCGCTTTATGAAGTGCTGACGGCGACGGCGGCGGTCA
GTAACCTGATTCGGGAAGGGAAAACGCACCAGCTTCCCGGCCTGATTCAAACCGGAGCCGCTGCGGGAATGCAAACGTTC
GAGCAAAGCTACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.751

97.654

0.496
  pilT Vibrio cholerae strain A1552

50.751

97.654

0.496
  pilT Neisseria meningitidis 8013

49.851

98.24

0.49
  pilT Neisseria gonorrhoeae MS11

49.552

98.24

0.487
  pilT Pseudomonas stutzeri DSM 10701

48.802

97.947

0.478
  pilT Acinetobacter baylyi ADP1

48.802

97.947

0.478
  pilT Acinetobacter nosocomialis M2

48.503

97.947

0.475
  pilT Legionella pneumophila strain Lp02

48.503

97.947

0.475
  pilT Legionella pneumophila strain ERS1305867

48.503

97.947

0.475
  pilT Pseudomonas aeruginosa PAK

48.503

97.947

0.475
  pilT Acinetobacter baumannii D1279779

48.503

97.947

0.475
  pilT Acinetobacter baumannii strain A118

48.503

97.947

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.567

100

0.437
  pilU Vibrio cholerae strain A1552

38.728

100

0.393
  pilU Pseudomonas stutzeri DSM 10701

39.583

98.534

0.39
  pilU Acinetobacter baylyi ADP1

38.272

95.015

0.364