Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H5A29_RS14615 Genome accession   NZ_CP059958
Coordinates   3292567..3293583 (+) Length   338 a.a.
NCBI ID   WP_119872027.1    Uniprot ID   -
Organism   Pectobacterium brasiliense strain IPO:4065 NAK:223     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3287567..3298583
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5A29_RS14580 (H5A29_14560) - 3287792..3288052 (-) 261 WP_015841595.1 type II toxin-antitoxin system ParD family antitoxin -
  H5A29_RS14585 (H5A29_14565) hemW 3288281..3289423 (-) 1143 WP_039530837.1 radical SAM family heme chaperone HemW -
  H5A29_RS14590 (H5A29_14570) - 3289416..3290009 (-) 594 WP_119872025.1 XTP/dITP diphosphatase -
  H5A29_RS14595 (H5A29_14575) yggU 3290027..3290317 (-) 291 WP_010279110.1 DUF167 family protein YggU -
  H5A29_RS14600 (H5A29_14580) - 3290314..3290868 (-) 555 WP_010279107.1 YggT family protein -
  H5A29_RS14605 (H5A29_14585) proC 3290917..3291738 (-) 822 WP_100018556.1 pyrroline-5-carboxylate reductase -
  H5A29_RS14610 (H5A29_14590) - 3291833..3292546 (-) 714 WP_119872026.1 YggS family pyridoxal phosphate-dependent enzyme -
  H5A29_RS14615 (H5A29_14595) pilT 3292567..3293583 (+) 1017 WP_119872027.1 type IV pilus twitching motility protein PilT Machinery gene
  H5A29_RS14620 (H5A29_14600) - 3293552..3295384 (-) 1833 WP_119872028.1 sensor domain-containing diguanylate cyclase -
  H5A29_RS14625 (H5A29_14605) - 3295911..3297482 (-) 1572 WP_119872029.1 methyl-accepting chemotaxis protein -
  H5A29_RS14630 (H5A29_14610) nsrR 3297791..3298216 (+) 426 WP_010279087.1 nitric oxide-sensing transcriptional repressor NsrR -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36611.80 Da        Isoelectric Point: 6.8190

>NTDB_id=472621 H5A29_RS14615 WP_119872027.1 3292567..3293583(+) (pilT) [Pectobacterium brasiliense strain IPO:4065 NAK:223]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTSDQVAQWCDAWLAPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSAQPSLAALQAPPVLSTLLEKPNGLILVTGATGSGKSTTLAAMLGALNDSSDRHVITLE
DPIEFIHASRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQRLFPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=472621 H5A29_RS14615 WP_119872027.1 3292567..3293583(+) (pilT) [Pectobacterium brasiliense strain IPO:4065 NAK:223]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCGGT
GCTGCGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGTCGGATCAGGTGGCACAATGGTGCGATG
CCTGGCTCGCACCCGCTCAGCGCGAGCAACTGCGGCAGGCTGGTCAGGTGGACGGCGCGCTAATGCTGCCCGATGGACAA
CGCCTGCGGGTCAATGTATTTCGCCAGCGGGAAGGGCTGTCTGCGGCGCTACGTATTATCCCATCCGCTCAGCCTTCATT
GGCGGCGCTACAGGCACCACCCGTTCTCTCGACGCTGCTGGAGAAACCGAACGGACTGATCCTAGTTACCGGTGCAACGG
GGAGCGGTAAATCCACGACGCTGGCGGCCATGCTTGGGGCGTTAAACGACAGCAGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTTATCCATGCCAGCCGACGTTGCCTGATTCAACAGAGGGAGATTGGTGCGCACAGTGCGTCCTTTGC
GCAGGCGCTACGTGCTGCATTGCGGGAGGATCCCGATGTTATTCTGCTGGGCGAATTGCGCGATACGGAGACGATTCGAC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGTTGTCGACGCTGCATACACGTAGTGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCCGGTGAAGAGAAAGCCTATGTTCGCAGCCAGTTAGCTACCTGTTTGCAGGCCGTGGTGACGCA
AAGGCTGTTTCCCGCTGCGCAAGGTGGTCGAATTGCGCTTTATGAAGTGCTGACGGCGACGGCGGCAGTCAGTAACCTGA
TTCGGGAAGGGAAAACGCACCAGCTTCCCGGCCTGATTCAAACCGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.212

97.633

0.5
  pilT Vibrio cholerae strain A1552

51.212

97.633

0.5
  pilT Neisseria meningitidis 8013

50.301

98.225

0.494
  pilT Neisseria gonorrhoeae MS11

50

98.225

0.491
  pilT Pseudomonas stutzeri DSM 10701

49.245

97.929

0.482
  pilT Pseudomonas aeruginosa PAK

49.245

97.929

0.482
  pilT Acinetobacter baylyi ADP1

48.943

97.929

0.479
  pilT Legionella pneumophila strain Lp02

48.943

97.929

0.479
  pilT Legionella pneumophila strain ERS1305867

48.943

97.929

0.479
  pilT Acinetobacter nosocomialis M2

48.64

97.929

0.476
  pilT Acinetobacter baumannii D1279779

48.64

97.929

0.476
  pilT Acinetobacter baumannii strain A118

48.64

97.929

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.433

99.704

0.423
  pilU Vibrio cholerae strain A1552

39.067

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

39.64

98.521

0.391
  pilU Acinetobacter baylyi ADP1

38.509

95.266

0.367