Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H5A38_RS04125 Genome accession   NZ_CP059956
Coordinates   922342..923358 (+) Length   338 a.a.
NCBI ID   WP_205546956.1    Uniprot ID   -
Organism   Pectobacterium brasiliense strain IPO:4132 NAK:239     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 917342..928358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5A38_RS04095 (H5A38_04095) hemW 918056..919198 (-) 1143 WP_205546958.1 radical SAM family heme chaperone HemW -
  H5A38_RS04100 (H5A38_04100) rdgB 919191..919784 (-) 594 WP_039497787.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  H5A38_RS04105 (H5A38_04105) yggU 919802..920092 (-) 291 WP_010279110.1 DUF167 family protein YggU -
  H5A38_RS04110 (H5A38_04110) - 920089..920643 (-) 555 WP_010295003.1 YggT family protein -
  H5A38_RS04115 (H5A38_04115) proC 920692..921513 (-) 822 WP_205547286.1 pyrroline-5-carboxylate reductase -
  H5A38_RS04120 (H5A38_04120) - 921608..922321 (-) 714 WP_205546957.1 YggS family pyridoxal phosphate-dependent enzyme -
  H5A38_RS04125 (H5A38_04125) pilT 922342..923358 (+) 1017 WP_205546956.1 type IV pilus twitching motility protein PilT Machinery gene
  H5A38_RS04130 (H5A38_04130) - 923327..925159 (-) 1833 WP_080163333.1 sensor domain-containing diguanylate cyclase -
  H5A38_RS04135 (H5A38_04135) - 925686..927257 (-) 1572 WP_205546955.1 methyl-accepting chemotaxis protein -
  H5A38_RS04140 (H5A38_04140) nsrR 927585..928010 (+) 426 WP_014916532.1 nitric oxide-sensing transcriptional repressor NsrR -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36635.82 Da        Isoelectric Point: 6.6533

>NTDB_id=472564 H5A38_RS04125 WP_205546956.1 922342..923358(+) (pilT) [Pectobacterium brasiliense strain IPO:4132 NAK:239]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTSDQVAQWCDAWLEPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSAQPSLAALQAPPVLSTLLEKPNGLILVTGATGSGKSTTLAAMLGALNDSSDRHVITLE
DPIEFIHASRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQRLLPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=472564 H5A38_RS04125 WP_205546956.1 922342..923358(+) (pilT) [Pectobacterium brasiliense strain IPO:4132 NAK:239]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCGGT
GCTGCGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGTCGGATCAGGTGGCACAATGGTGCGATG
CCTGGCTCGAACCCGCTCAGAGAGAGCAACTGCGGCAGGCTGGGCAGGTGGACGGGGCATTAATGCTGCCCGATGGACAA
CGCCTGCGGGTGAATGTGTTTCGTCAGCGGGAAGGGCTGTCTGCGGCGCTACGTATTATCCCGTCCGCTCAGCCTTCATT
GGCGGCGCTACAGGCACCACCCGTTCTCTCGACGCTGCTGGAGAAACCGAACGGACTGATCCTAGTTACCGGTGCAACGG
GGAGCGGTAAATCTACGACGCTGGCGGCCATGCTTGGGGCGTTAAACGACAGCAGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTTATCCATGCCAGCCGACGTTGCCTGATTCAACAGAGGGAGATTGGTGCACACAGTGCGTCCTTTGC
GCAGGCGCTACGGGCCGCATTGCGGGAGGATCCCGATGTCATTCTGCTGGGCGAATTGCGCGATACGGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGTTGTCGACGCTGCATACACGTAGTGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCCGGTGAAGAGAAAGCCTATGTTCGCAGCCAGTTAGCTACCTGCTTGCAGGCCGTGGTGACGCA
AAGGCTGTTGCCCGCTGCGCAAGGTGGCCGAATTGCGCTTTATGAAGTGCTGACGGCGACGGCGGCGGTCAGTAACCTGA
TTCGGGAAGGGAAAACGCACCAGCTTCCCGGCCTGATTCAAACCGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.515

97.633

0.503
  pilT Vibrio cholerae strain A1552

51.515

97.633

0.503
  pilT Neisseria meningitidis 8013

50.602

98.225

0.497
  pilT Neisseria gonorrhoeae MS11

50.301

98.225

0.494
  pilT Pseudomonas stutzeri DSM 10701

49.547

97.929

0.485
  pilT Pseudomonas aeruginosa PAK

49.245

97.929

0.482
  pilT Legionella pneumophila strain Lp02

49.245

97.929

0.482
  pilT Legionella pneumophila strain ERS1305867

49.245

97.929

0.482
  pilT Acinetobacter baylyi ADP1

49.245

97.929

0.482
  pilT Acinetobacter nosocomialis M2

48.943

97.929

0.479
  pilT Acinetobacter baumannii D1279779

48.943

97.929

0.479
  pilT Acinetobacter baumannii strain A118

48.943

97.929

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.73

99.704

0.426
  pilU Vibrio cholerae strain A1552

39.359

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

39.64

98.521

0.391
  pilU Acinetobacter baylyi ADP1

38.509

95.266

0.367