Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EJP617_RS08760 Genome accession   NC_017445
Coordinates   1926581..1927588 (+) Length   335 a.a.
NCBI ID   WP_041474411.1    Uniprot ID   -
Organism   Erwinia sp. Ejp617     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1921581..1932588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJP617_RS20180 - 1921759..1921995 (+) 237 Protein_1707 IS1 family transposase -
  EJP617_RS08735 (EJP617_17440) - 1922156..1923385 (+) 1230 WP_014543299.1 trypsin-like peptidase domain-containing protein -
  EJP617_RS08740 (EJP617_17450) hemW 1923450..1924586 (-) 1137 WP_014543300.1 radical SAM family heme chaperone HemW -
  EJP617_RS08745 (EJP617_17460) rdgB 1924579..1925172 (-) 594 WP_014543301.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  EJP617_RS08750 (EJP617_17470) - 1925304..1925843 (-) 540 WP_004168536.1 YggT family protein -
  EJP617_RS08755 (EJP617_17480) - 1925852..1926565 (-) 714 WP_041474410.1 YggS family pyridoxal phosphate-dependent enzyme -
  EJP617_RS08760 (EJP617_17490) pilT 1926581..1927588 (+) 1008 WP_041474411.1 type IV pilus twitching motility protein PilT Machinery gene
  EJP617_RS08765 (EJP617_17500) - 1927598..1928239 (-) 642 WP_014543304.1 NrsF family protein -
  EJP617_RS08770 (EJP617_17510) - 1928226..1928792 (-) 567 WP_014543305.1 sigma-70 family RNA polymerase sigma factor -
  EJP617_RS08775 (EJP617_17520) - 1928875..1930113 (-) 1239 WP_014543306.1 thioredoxin family protein -
  EJP617_RS08780 (EJP617_17530) - 1930139..1930702 (-) 564 WP_014543307.1 peroxiredoxin -
  EJP617_RS08785 (EJP617_17540) ruvX 1930844..1931269 (-) 426 WP_014543308.1 Holliday junction resolvase RuvX -
  EJP617_RS08790 (EJP617_17550) - 1931266..1931829 (-) 564 WP_012669218.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 36543.94 Da        Isoelectric Point: 6.9260

>NTDB_id=47204 EJP617_RS08760 WP_041474411.1 1926581..1927588(+) (pilT) [Erwinia sp. Ejp617]
MDIEDMVALSVKHNAGDLHLCSGHSPFWRCDGRLEAMADRPVIQDKWLESVAARWLSSAQHQELTLNGQVDFALTLGDGV
RLRANLFRQRYGLSLALRLIASQCPPLASLHLPQVTQSLLQVEEGLILITGATGSGKSTTLAALIDEINQRQRRHILTLE
DPVEFLHHSAQSLIQQREKGQHFTRFEAGLRAALREDPDVILLGELRDAETIRLALTAAETGHLVLATLHTRGAAQAVDR
LVDVFPGDEKNLVRSQLAGSLQAVIAQRLVAAVQGRVALFEVLVNGPAVANLIREGKTHQLPGLMQTGCQQGMQTFEQSL
AGRQKMGMVAISPSA

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=47204 EJP617_RS08760 WP_041474411.1 1926581..1927588(+) (pilT) [Erwinia sp. Ejp617]
ATGGATATCGAGGACATGGTGGCCCTTAGTGTAAAGCATAATGCGGGGGATCTGCACCTCTGCAGTGGACATTCGCCATT
CTGGCGCTGTGATGGGCGCCTGGAGGCGATGGCCGATCGGCCCGTCATACAGGATAAATGGCTGGAAAGCGTTGCTGCGC
GCTGGCTAAGTTCCGCTCAACATCAGGAATTGACGCTGAACGGCCAGGTGGATTTTGCCCTTACGCTGGGCGACGGCGTG
CGTCTGCGCGCCAATCTTTTTCGTCAGCGGTACGGGCTGTCGCTGGCGCTGCGTCTGATTGCCAGCCAGTGCCCGCCGCT
GGCATCATTGCACCTACCGCAAGTCACTCAATCTCTGTTACAGGTGGAAGAGGGGCTTATTCTGATTACCGGCGCGACCG
GCAGCGGCAAGTCGACCACGCTGGCCGCGCTGATCGACGAGATCAACCAGCGGCAGCGGCGGCATATTCTGACGCTGGAA
GACCCGGTGGAATTTCTCCATCACAGCGCGCAATCGCTGATCCAGCAGCGTGAGAAAGGCCAGCATTTTACCCGCTTTGA
AGCTGGACTGCGTGCGGCGTTGCGCGAAGATCCCGATGTGATCCTGCTGGGGGAGCTGCGCGATGCGGAAACCATCCGGC
TGGCGTTGACGGCCGCCGAGACCGGGCATCTGGTGCTGGCAACGCTGCATACCCGTGGTGCCGCGCAGGCGGTGGATCGG
CTGGTGGATGTTTTTCCGGGCGATGAAAAAAATTTGGTACGCAGCCAGCTGGCGGGAAGCTTACAGGCGGTGATTGCCCA
GCGCCTGGTGGCAGCCGTTCAGGGCAGGGTGGCGCTGTTTGAAGTGCTGGTGAACGGCCCGGCGGTGGCTAATTTAATCC
GCGAAGGGAAAACCCATCAGCTGCCCGGCCTGATGCAGACCGGCTGCCAGCAGGGGATGCAGACCTTCGAGCAAAGCCTG
GCCGGGCGGCAAAAGATGGGGATGGTAGCTATCTCGCCGTCGGCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(60-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

48.494

99.104

0.481
  pilT Neisseria gonorrhoeae MS11

48.193

99.104

0.478
  pilT Vibrio cholerae strain A1552

48.438

95.522

0.463
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.438

95.522

0.463
  pilT Pseudomonas stutzeri DSM 10701

45.619

98.806

0.451
  pilT Acinetobacter baylyi ADP1

44.94

100

0.451
  pilT Acinetobacter baumannii D1279779

45.317

98.806

0.448
  pilT Acinetobacter baumannii strain A118

45.317

98.806

0.448
  pilT Acinetobacter nosocomialis M2

45.317

98.806

0.448
  pilT Legionella pneumophila strain ERS1305867

46.106

95.821

0.442
  pilT Legionella pneumophila strain Lp02

46.106

95.821

0.442
  pilT Pseudomonas aeruginosa PAK

44.713

98.806

0.442
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.444

96.716

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.062

96.119

0.385
  pilU Acinetobacter baylyi ADP1

38.226

97.612

0.373
  pilU Vibrio cholerae strain A1552

39.032

92.537

0.361

Multiple sequence alignment