Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H3303_RS29135 Genome accession   NZ_CP059738
Coordinates   6575159..6576193 (-) Length   344 a.a.
NCBI ID   WP_007953348.1    Uniprot ID   J3FB13
Organism   Pseudomonas sp. 29A     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6570159..6581193
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3303_RS29115 (H3303_29115) - 6570612..6571883 (+) 1272 WP_175555546.1 dihydroorotase -
  H3303_RS29120 (H3303_29120) - 6572206..6573192 (+) 987 WP_016985975.1 lipid II-degrading bacteriocin -
  H3303_RS29125 (H3303_29125) - 6573710..6574141 (-) 432 WP_122596578.1 TM2 domain-containing protein -
  H3303_RS29130 (H3303_29130) - 6574390..6575007 (+) 618 WP_016985978.1 C40 family peptidase -
  H3303_RS29135 (H3303_29135) pilT 6575159..6576193 (-) 1035 WP_007953348.1 type IV pilus twitching motility protein PilT Machinery gene
  H3303_RS29140 (H3303_29140) - 6576250..6576936 (+) 687 WP_175555547.1 YggS family pyridoxal phosphate-dependent enzyme -
  H3303_RS29145 (H3303_29145) proC 6576960..6577778 (+) 819 WP_175555548.1 pyrroline-5-carboxylate reductase -
  H3303_RS29150 (H3303_29150) - 6577789..6578379 (+) 591 WP_095186992.1 YggT family protein -
  H3303_RS29155 (H3303_29155) - 6578376..6578672 (+) 297 WP_175555549.1 DUF167 domain-containing protein -
  H3303_RS29160 (H3303_29160) - 6579106..6580245 (+) 1140 WP_016983244.1 homoserine O-acetyltransferase -
  H3303_RS29165 (H3303_29165) metW 6580253..6580873 (+) 621 WP_007913638.1 methionine biosynthesis protein MetW -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38071.74 Da        Isoelectric Point: 7.0157

>NTDB_id=471702 H3303_RS29135 WP_007953348.1 6575159..6576193(-) (pilT) [Pseudomonas sp. 29A]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLAMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVTKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=471702 H3303_RS29135 WP_007953348.1 6575159..6576193(-) (pilT) [Pseudomonas sp. 29A]
ATGGATATCACTGAACTGCTGGCCTTCAGCGCCAAACAGGGAGCTTCCGACCTGCACCTGTCGGCCGGTCTGCCGCCGAT
GATCCGTGTCGATGGCGATGTGCGGCGGATCAATCTGCCAGCGCTCGACCACAAGCAAGTGCACGAACTGATCTACGACA
TCATGAACGACACCCAGCGCGTCGACTTCGAGAAACATCTGGAAACCGACTTTTCCTTCGAAGTGCCGGGTGTCGCGCGT
TTCCGGGTTAATGCCTTTAACCAGAACCGAGGTGCGGGCGCCGTATTCCGTACGATTCCGTCGAAAGTGCTGAGCATGGA
AGACTTAGCCATGGGCGATGTCTTTCGCAAGATCACCGATGCGCCGCGAGGGCTGGTGCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACACTGGCGGCGATGATCGACTACCTCAATACCCATCGCCACCACCACATTCTGACGATTGAAGAC
CCGATCGAATTTGTTCATGAGTCGCGCAAATGCCTGATCAATCAGCGCGAAGTTCACCGTGATACCCGCAGTTTCGCTAC
GGCTTTGCGTTCAGCGCTGCGCGAAGACCCGGATGTGATTCTGGTTGGCGAGATGCGTGACCTGGAAACCATTCGCCTGG
CGCTGACCGCTGCCGAGACTGGGCATTTGGTGTTCGGCACCTTGCACACCACGTCGGCGGCGAAAACCATCGACCGCGTG
GTCGACGTATTCCCCGGTGACGAGAAGTCGATGGTGCGCTCGATGCTGTCGGAGTCGTTACTGGCGGTGGTCTCGCAGAC
GTTGATCAAGAAGATCGGCGGCGGGCGGGTGGCGGCGCACGAGATCATGCTGGGGACGTCAGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATTCAGACGGGGGGATCGTTGGGGATGCAGACACTGGACATGTGCCTG
AAGGATCTGGTGACCAAGGGTTTGATCAGTCGCGAACATGCGCGGGAGAAGGCACGCACGCCGGATAATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J3FB13

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

87.791

100

0.878
  pilT Pseudomonas stutzeri DSM 10701

87.791

100

0.878
  pilT Acinetobacter nosocomialis M2

79.651

100

0.797
  pilT Acinetobacter baumannii D1279779

79.651

100

0.797
  pilT Acinetobacter baumannii strain A118

79.651

100

0.797
  pilT Acinetobacter baylyi ADP1

77.616

100

0.776
  pilT Legionella pneumophila strain Lp02

72.485

98.256

0.712
  pilT Legionella pneumophila strain ERS1305867

72.485

98.256

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

39.71

100

0.398