Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilT   Type   Machinery gene
Locus tag   H0H24_RS17770 Genome accession   NZ_CP059729
Coordinates   3738713..3739750 (-) Length   345 a.a.
NCBI ID   WP_000355489.1    Uniprot ID   A0A009PTR1
Organism   Acinetobacter baumannii strain AbCTX13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 3715100..3801583 3738713..3739750 within 0


Gene organization within MGE regions


Location: 3715100..3801583
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0H24_RS17650 (H0H24_17625) - 3715129..3715944 (-) 816 WP_024432392.1 arginyltransferase -
  H0H24_RS17655 (H0H24_17630) aat 3715963..3716694 (-) 732 WP_000854785.1 leucyl/phenylalanyl-tRNA--protein transferase -
  H0H24_RS17660 (H0H24_17635) trxB 3716814..3717761 (-) 948 WP_001276144.1 thioredoxin-disulfide reductase -
  H0H24_RS17665 (H0H24_17640) - 3718031..3721063 (+) 3033 WP_000127827.1 DNA translocase FtsK -
  H0H24_RS17670 (H0H24_17645) rhtC 3721212..3721832 (+) 621 WP_000959259.1 threonine export protein RhtC -
  H0H24_RS17675 (H0H24_17650) - 3721875..3722162 (-) 288 WP_001218560.1 PA4642 family protein -
  H0H24_RS17680 (H0H24_17655) minE 3722246..3722518 (-) 273 WP_000896934.1 cell division topological specificity factor MinE -
  H0H24_RS17685 (H0H24_17660) minD 3722521..3723333 (-) 813 WP_001074362.1 septum site-determining protein MinD -
  H0H24_RS17690 (H0H24_17665) minC 3723404..3724126 (-) 723 WP_000763675.1 septum site-determining protein MinC -
  H0H24_RS17695 (H0H24_17670) - 3724248..3725294 (-) 1047 WP_001181638.1 hypothetical protein -
  H0H24_RS17700 (H0H24_17675) - 3725310..3726236 (-) 927 WP_000100973.1 acyltransferase -
  H0H24_RS17705 (H0H24_17680) - 3726481..3727134 (+) 654 WP_001202415.1 OmpA family protein -
  H0H24_RS17710 (H0H24_17685) rep 3727328..3729367 (+) 2040 WP_000093035.1 DNA helicase Rep -
  H0H24_RS17715 (H0H24_17690) dut 3729391..3729843 (+) 453 WP_000868152.1 dUTP diphosphatase -
  H0H24_RS17720 (H0H24_17695) - 3730043..3731461 (+) 1419 WP_001102845.1 phosphomannomutase/phosphoglucomutase -
  H0H24_RS17725 (H0H24_17700) argB 3731476..3732384 (+) 909 WP_001135419.1 acetylglutamate kinase -
  H0H24_RS17730 (H0H24_17705) - 3732505..3733287 (+) 783 WP_000890284.1 GNAT family N-acetyltransferase -
  H0H24_RS17735 (H0H24_17710) - 3733396..3734244 (+) 849 WP_000336554.1 class II glutamine amidotransferase -
  H0H24_RS17740 (H0H24_17715) - 3734497..3734952 (+) 456 WP_000782976.1 bacteriohemerythrin -
  H0H24_RS17745 (H0H24_17720) - 3735105..3736172 (+) 1068 WP_029776981.1 membrane protein -
  H0H24_RS17750 (H0H24_17725) - 3736172..3736495 (+) 324 WP_000442585.1 RnfH family protein -
  H0H24_RS17755 (H0H24_17730) - 3736550..3736948 (-) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  H0H24_RS17760 (H0H24_17735) fur 3737060..3737497 (+) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  H0H24_RS17765 (H0H24_17740) pilU 3737567..3738685 (-) 1119 WP_000347036.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H0H24_RS17770 (H0H24_17745) pilT 3738713..3739750 (-) 1038 WP_000355489.1 type IV pilus twitching motility protein PilT Machinery gene
  H0H24_RS17775 (H0H24_17750) - 3739877..3740569 (+) 693 WP_024432389.1 YggS family pyridoxal phosphate-dependent enzyme -
  H0H24_RS17780 (H0H24_17755) - 3740770..3744366 (-) 3597 WP_000698797.1 AAA family ATPase -
  H0H24_RS17785 (H0H24_17760) - 3744376..3745644 (-) 1269 WP_032061991.1 exonuclease SbcCD subunit D -
  H0H24_RS17790 (H0H24_17765) - 3745808..3746281 (+) 474 WP_000974354.1 OsmC family protein -
  H0H24_RS17795 (H0H24_17770) - 3746355..3746732 (-) 378 WP_001091956.1 VOC family protein -
  H0H24_RS17800 (H0H24_17775) - 3747112..3747564 (+) 453 WP_000164152.1 ABZJ_00895 family protein -
  H0H24_RS17805 (H0H24_17780) - 3747654..3748559 (+) 906 WP_001039349.1 TIGR01777 family oxidoreductase -
  H0H24_RS17810 (H0H24_17785) - 3748551..3749666 (-) 1116 WP_000546678.1 FAD-binding oxidoreductase -
  H0H24_RS17815 (H0H24_17790) hemB 3749692..3750705 (-) 1014 WP_004748084.1 porphobilinogen synthase -
  H0H24_RS17820 (H0H24_17795) - 3750848..3751345 (+) 498 WP_010590410.1 thioesterase family protein -
  H0H24_RS17825 (H0H24_17800) - 3751615..3752766 (+) 1152 WP_000128703.1 EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit -
  H0H24_RS17830 (H0H24_17805) - 3752773..3754296 (+) 1524 WP_000857095.1 DHA2 family efflux MFS transporter permease subunit -
  H0H24_RS17835 (H0H24_17810) ggt 3754600..3756585 (+) 1986 WP_001042935.1 gamma-glutamyltransferase -
  H0H24_RS17845 (H0H24_17820) - 3757288..3758517 (+) 1230 WP_032071232.1 tyrosine-type recombinase/integrase -
  H0H24_RS17850 (H0H24_17825) - 3758667..3759293 (+) 627 WP_000151566.1 hypothetical protein -
  H0H24_RS17855 (H0H24_17830) - 3759421..3759636 (+) 216 WP_000153154.1 hypothetical protein -
  H0H24_RS17860 (H0H24_17835) - 3759639..3759848 (+) 210 WP_001016479.1 helix-turn-helix domain-containing protein -
  H0H24_RS17865 (H0H24_17840) - 3759845..3760399 (+) 555 WP_002003158.1 BRO family protein -
  H0H24_RS17870 (H0H24_17845) - 3760392..3760757 (+) 366 WP_001046482.1 hypothetical protein -
  H0H24_RS19875 - 3760770..3761072 (+) 303 WP_000787402.1 hypothetical protein -
  H0H24_RS17875 (H0H24_17850) - 3761187..3761465 (+) 279 WP_001064707.1 hypothetical protein -
  H0H24_RS17880 (H0H24_17855) - 3761477..3763909 (+) 2433 Protein_3510 DUF927 domain-containing protein -
  H0H24_RS17885 (H0H24_17860) - 3764242..3764445 (+) 204 WP_001984265.1 single-stranded DNA-binding protein -
  H0H24_RS17890 (H0H24_17865) - 3764582..3766024 (+) 1443 WP_000003532.1 hypothetical protein -
  H0H24_RS17895 (H0H24_17870) - 3766367..3767041 (+) 675 WP_000560650.1 DUF3800 domain-containing protein -
  H0H24_RS17900 (H0H24_17875) - 3767041..3767229 (+) 189 WP_000642900.1 hypothetical protein -
  H0H24_RS17905 (H0H24_17880) - 3767285..3767950 (+) 666 WP_000515964.1 hypothetical protein -
  H0H24_RS17915 (H0H24_17890) - 3768924..3769970 (+) 1047 WP_000027065.1 nucleoid-associated protein -
  H0H24_RS17920 (H0H24_17895) - 3769973..3771178 (+) 1206 WP_024432374.1 hypothetical protein -
  H0H24_RS17925 (H0H24_17900) tnpC 3771333..3772892 (-) 1560 Protein_3518 IS66 family transposase -
  H0H24_RS17930 (H0H24_17905) tnpB 3773023..3773298 (-) 276 WP_203463190.1 IS66 family insertion sequence element accessory protein TnpB -
  H0H24_RS17935 (H0H24_17910) - 3773295..3773676 (-) 382 Protein_3520 transposase -
  H0H24_RS17940 (H0H24_17915) tnpC 3775557..3777115 (-) 1559 Protein_3521 IS66 family transposase -
  H0H24_RS17945 (H0H24_17920) tnpB 3777244..3777516 (-) 273 WP_203463191.1 IS66 family insertion sequence element accessory protein TnpB -
  H0H24_RS19880 - 3777513..3777895 (-) 383 Protein_3523 transposase -
  H0H24_RS17955 (H0H24_17930) - 3778012..3779559 (+) 1548 WP_000124669.1 class I SAM-dependent DNA methyltransferase -
  H0H24_RS17960 (H0H24_17935) - 3779556..3780701 (+) 1146 WP_000637753.1 restriction endonuclease subunit S -
  H0H24_RS17965 (H0H24_17940) - 3780752..3781291 (+) 540 WP_225480425.1 DUF2939 domain-containing protein -
  H0H24_RS17970 (H0H24_17945) - 3781593..3784862 (+) 3270 WP_024432358.1 type I restriction endonuclease subunit R -
  H0H24_RS17975 (H0H24_17950) - 3785348..3786820 (-) 1473 WP_000626990.1 N-6 DNA methylase -
  H0H24_RS17980 (H0H24_17955) - 3786804..3787409 (-) 606 WP_000477039.1 restriction endonuclease subunit S -
  H0H24_RS17985 (H0H24_17960) - 3787429..3787611 (-) 183 WP_000797544.1 hypothetical protein -
  H0H24_RS17990 (H0H24_17965) - 3787967..3788881 (+) 915 WP_024432357.1 GIY-YIG nuclease family protein -
  H0H24_RS17995 (H0H24_17970) - 3789150..3789434 (-) 285 WP_000675260.1 hypothetical protein -
  H0H24_RS18000 (H0H24_17975) - 3790072..3791418 (-) 1347 WP_024432356.1 OprD family outer membrane porin -
  H0H24_RS18005 (H0H24_17980) - 3791497..3792882 (-) 1386 WP_000182789.1 MFS transporter -
  H0H24_RS18010 (H0H24_17985) - 3793055..3793999 (-) 945 WP_000050872.1 dihydrodipicolinate synthase family protein -
  H0H24_RS18015 (H0H24_17990) - 3794186..3795076 (+) 891 WP_001105541.1 LysR family transcriptional regulator -
  H0H24_RS18020 (H0H24_17995) - 3795213..3795734 (+) 522 WP_003653184.1 sigma-70 family RNA polymerase sigma factor -
  H0H24_RS18025 (H0H24_18000) - 3795735..3796751 (+) 1017 WP_001240510.1 FecR domain-containing protein -
  H0H24_RS18030 (H0H24_18005) - 3796891..3800064 (+) 3174 WP_004736030.1 TonB-dependent receptor domain-containing protein -
  H0H24_RS18035 (H0H24_18010) - 3800255..3801046 (+) 792 WP_002004304.1 Slam-dependent surface lipoprotein -

Sequence


Protein


Download         Length: 345 a.a.        Molecular weight: 38312.92 Da        Isoelectric Point: 6.7271

>NTDB_id=471623 H0H24_RS17770 WP_000355489.1 3738713..3739750(-) (pilT) [Acinetobacter baumannii strain AbCTX13]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KGLVARGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=471623 H0H24_RS17770 WP_000355489.1 3738713..3739750(-) (pilT) [Acinetobacter baumannii strain AbCTX13]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCGTCCGATTTACATCTCTCTGCTGGCATGCCACCAAT
GATTCGTGTTGACGGTGAAGTTCGTCGTATTAACTTACCAGCCTTAGAACATAAAGATGTCCACCGTCTTGTTTACGACA
TCATGAATGATAAACAGCGCCGTGACTATGAAGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCTAATGTTGCGCGT
TTCCGTGTTAACGCATTTAACCAAAACCGTGGTGCTGGTGCAGTATTTCGTACCATTCCATCTAAAGTCCTCACCATGGA
AGATCTAGGTCTAGGACAAATCTTTAAAGATATTTGCGACTATCCTCGTGGCATTGTACTGGTGACAGGACCAACAGGTT
CGGGTAAATCAACTACGCTTGCAGCGATGCTCGATTATATTAACGAAAACCGCTATGACCATATTTTAACGGTGGAAGAT
CCAATCGAATTTGTACATCAATCTAAGAAATGTCTCATTAACCAGCGTGAAGTGCATCGTGACACACACGGCTTTAATGA
AGCACTTCGCTCAGCACTGCGTGAAGACCCTGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAAACTATTCGCTTGG
CATTAACCGCTGCCGAAACAGGTCACTTGGTGTTTGGTACACTCCACACCACGTCGGCAGCAAAAACTATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTTCGTGCCATGCTATCAGAATCTTTACAAGCGGTTATTTCACAAAC
CCTGCTTAAAAAGAATGGTGGTGGCCGTGTCGCAGCACATGAGATCATGATTGGTATTCCGGCTATTCGTAACTTAATTC
GTGAAAACAAAGTCGCGCAAATGTACTCAGCCATTCAAACTGGTGCCAATCATGGCATGACTACCCTCGACCAAAGTTTA
AAAGGCTTAGTCGCTCGCGGTGTGATTAGTCCACAAACCGCACGTACTGCGGCAAAACAGCCTGAATCATTCTTATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A009PTR1

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

100

100

1

  pilT Acinetobacter baumannii D1279779

100

100

1

  pilT Acinetobacter nosocomialis M2

99.71

100

0.997

  pilT Acinetobacter baylyi ADP1

88.953

99.71

0.887

  pilT Pseudomonas aeruginosa PAK

84.302

99.71

0.841

  pilT Pseudomonas stutzeri DSM 10701

84.012

99.71

0.838

  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754

  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754

  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.726

97.391

0.699

  pilT Vibrio cholerae strain A1552

71.726

97.391

0.699

  pilT Neisseria meningitidis 8013

68.116

100

0.681

  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678

  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522

  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409

  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394

  pilU Acinetobacter baylyi ADP1

38.728

100

0.388