Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HBNCFIEN_RS05145 Genome accession   NZ_CP059576
Coordinates   1236469..1237503 (+) Length   344 a.a.
NCBI ID   WP_182393006.1    Uniprot ID   A0A7G4REP7
Organism   Legionella sp. PC997     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1231469..1242503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBNCFIEN_RS05115 (HBNCFIEN_01033) - 1231860..1232054 (-) 195 WP_182393000.1 hypothetical protein -
  HBNCFIEN_RS05120 (HBNCFIEN_01034) - 1232193..1233638 (-) 1446 WP_182393001.1 hypothetical protein -
  HBNCFIEN_RS05125 (HBNCFIEN_01035) - 1233947..1234228 (-) 282 WP_182393002.1 hypothetical protein -
  HBNCFIEN_RS05130 (HBNCFIEN_01036) - 1234390..1234956 (-) 567 WP_182393003.1 DUF2845 domain-containing protein -
  HBNCFIEN_RS05135 (HBNCFIEN_01037) - 1235003..1235578 (-) 576 WP_182393004.1 YggT family protein -
  HBNCFIEN_RS05140 (HBNCFIEN_01038) - 1235635..1236321 (-) 687 WP_182393005.1 YggS family pyridoxal phosphate-dependent enzyme -
  HBNCFIEN_RS05145 (HBNCFIEN_01039) pilT 1236469..1237503 (+) 1035 WP_182393006.1 type IV pilus twitching motility protein PilT Machinery gene
  HBNCFIEN_RS05150 (HBNCFIEN_01040) rph 1237713..1238420 (-) 708 WP_182393007.1 ribonuclease PH -
  HBNCFIEN_RS05155 - 1238676..1238813 (+) 138 WP_182393008.1 hypothetical protein -
  HBNCFIEN_RS05160 (HBNCFIEN_01041) - 1238900..1239769 (+) 870 WP_182393009.1 MC/SLC25 family protein -
  HBNCFIEN_RS05165 (HBNCFIEN_01042) - 1239964..1240830 (+) 867 WP_182393010.1 YicC/YloC family endoribonuclease -
  HBNCFIEN_RS05170 (HBNCFIEN_01043) gmk 1240836..1241465 (+) 630 WP_182393615.1 guanylate kinase -
  HBNCFIEN_RS05175 (HBNCFIEN_01044) rpoZ 1241497..1241700 (+) 204 WP_010653170.1 DNA-directed RNA polymerase subunit omega -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38369.35 Da        Isoelectric Point: 6.3136

>NTDB_id=470902 HBNCFIEN_RS05145 WP_182393006.1 1236469..1237503(+) (pilT) [Legionella sp. PC997]
MDIAELLAFSVKNKSSDLHLSAGLPPMIRVDGDLRKINLPALEHKEVMKIVYDVMNDRQRKEFEEYLEVDFSFEIANLAR
FRVNVFNQLRGAAAVFRTIPSEILGMDDLGLPPIFEEMASYPKGLVLITGPTGSGKSTTLAAIIDYINANRYDHILTVED
PIEFVHQSKKCLLNQREVHRDTLSFNAALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTNSATKTINRI
IDVFPAEEKSMVRSMLSESLQAVVAQTLLKKNGGGRVAALEIMICTGAIRNLIREDKVAQMYSSIQTGQGKGMQTLDQHL
TELVKKNIISIYTAREVAIDKNLF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=470902 HBNCFIEN_RS05145 WP_182393006.1 1236469..1237503(+) (pilT) [Legionella sp. PC997]
ATGGACATAGCGGAACTATTGGCGTTTTCAGTAAAAAATAAATCCTCAGACTTACATCTTTCGGCAGGTTTGCCTCCAAT
GATTCGAGTTGATGGGGATTTACGTAAAATCAATTTACCCGCCCTGGAGCATAAAGAGGTAATGAAAATTGTCTATGATG
TAATGAATGACAGGCAGAGAAAAGAATTTGAAGAATATTTAGAGGTTGATTTTTCTTTTGAAATCGCAAATCTAGCCCGT
TTTAGGGTGAATGTGTTTAATCAATTACGTGGCGCCGCGGCAGTTTTTCGTACCATCCCTTCCGAAATTTTAGGCATGGA
TGATCTAGGACTACCTCCTATTTTTGAAGAAATGGCTAGTTATCCTAAGGGATTAGTATTGATAACTGGTCCTACAGGCT
CAGGTAAAAGTACTACATTGGCTGCGATCATCGATTACATTAATGCAAATCGTTACGATCATATTTTAACAGTTGAAGAT
CCTATAGAGTTTGTACATCAAAGTAAGAAATGTTTGTTAAACCAACGGGAAGTTCATCGTGATACGCTGAGTTTTAATGC
GGCTTTACGTTCGGCACTTCGCGAGGATCCGGATGTGATTCTAGTCGGGGAATTGCGGGATTTGGAGACAATCCGCTTGG
CAATGACTGCTGCGGAAACCGGACACCTTGTATTTGGGACCTTACATACCAATTCAGCAACCAAAACCATCAATAGAATT
ATTGATGTGTTTCCTGCTGAAGAAAAATCGATGGTGCGTTCTATGTTATCCGAATCTTTGCAAGCCGTAGTAGCACAAAC
TTTATTGAAAAAAAATGGTGGGGGACGGGTTGCTGCTTTGGAAATTATGATTTGTACCGGTGCGATACGAAACTTAATTC
GTGAAGATAAAGTGGCACAAATGTACTCTTCAATCCAAACAGGTCAAGGCAAAGGAATGCAAACTTTGGATCAGCATTTA
ACTGAGTTGGTGAAGAAAAATATTATCTCTATTTATACAGCACGTGAAGTGGCTATTGATAAAAATTTATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G4REP7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

88.372

100

0.884
  pilT Legionella pneumophila strain ERS1305867

88.372

100

0.884
  pilT Acinetobacter nosocomialis M2

77.219

98.256

0.759
  pilT Acinetobacter baumannii D1279779

77.219

98.256

0.759
  pilT Acinetobacter baumannii strain A118

77.219

98.256

0.759
  pilT Acinetobacter baylyi ADP1

76.331

98.256

0.75
  pilT Pseudomonas stutzeri DSM 10701

74.556

98.256

0.733
  pilT Pseudomonas aeruginosa PAK

74.26

98.256

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.389

95.64

0.654
  pilT Vibrio cholerae strain A1552

68.389

95.64

0.654
  pilT Neisseria meningitidis 8013

65.487

98.547

0.645
  pilT Neisseria gonorrhoeae MS11

65.192

98.547

0.642
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.5
  pilU Vibrio cholerae strain A1552

41.994

96.221

0.404
  pilU Pseudomonas stutzeri DSM 10701

42.593

94.186

0.401
  pilU Acinetobacter baylyi ADP1

38.663

100

0.387