Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H3L92_RS08370 Genome accession   NZ_CP059570
Coordinates   1768574..1769701 (+) Length   375 a.a.
NCBI ID   WP_085365174.1    Uniprot ID   A0A1X3DEU8
Organism   Neisseria dentiae strain DSM 19151     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1763574..1774701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3L92_RS08355 (H3L92_08355) - 1764584..1765603 (+) 1020 WP_085365171.1 lysozyme inhibitor LprI family protein -
  H3L92_RS08360 (H3L92_08360) - 1765906..1767375 (-) 1470 WP_085365172.1 DASS family sodium-coupled anion symporter -
  H3L92_RS08365 (H3L92_08365) - 1767552..1768265 (-) 714 WP_085365243.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  H3L92_RS08370 (H3L92_08370) pilU 1768574..1769701 (+) 1128 WP_085365174.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H3L92_RS08375 (H3L92_08375) tig 1769945..1771255 (+) 1311 WP_085365175.1 trigger factor -
  H3L92_RS08380 (H3L92_08380) clpP 1771454..1772080 (+) 627 WP_085365176.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -
  H3L92_RS08385 (H3L92_08385) murJ 1772323..1773867 (+) 1545 WP_085365177.1 murein biosynthesis integral membrane protein MurJ -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 42213.42 Da        Isoelectric Point: 6.3463

>NTDB_id=470835 H3L92_RS08370 WP_085365174.1 1768574..1769701(+) (pilU) [Neisseria dentiae strain DSM 19151]
MNENPLPAKEEIYSLLKTLNKYKGSDLFITANFPPAMKLDGKITRITETPLTPERCMEIAFSIMSPKQMDEFMNTNECNF
AISLPDTSRFRVNAMVQRGATALVFRTITSNIPKFETLNLPPVLQKIAMQKRGLVIFVGGTGSGKSTSLASMIDYRNENS
HDHIITIEDPIEFVHQHKNCIITQREVGVDTENWFAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHAN
STNQALDRIINFFPEERRVQLLTDLSLNLQAFISQRLVPREGGKGRVAAVEVLLNSPLIAELIHNGDVHSIKEIMKKSQS
MGMQTFDQALYELYENGHISFQDAIKNADSAHDLRLDIQLRSRRAQNAGPDLELI

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=470835 H3L92_RS08370 WP_085365174.1 1768574..1769701(+) (pilU) [Neisseria dentiae strain DSM 19151]
ATGAACGAGAATCCGCTGCCGGCCAAAGAAGAAATCTATTCATTACTGAAAACACTCAACAAATACAAAGGCTCCGACCT
TTTCATCACCGCCAACTTTCCGCCGGCCATGAAGCTCGACGGCAAAATCACGCGCATCACCGAAACACCGCTCACGCCCG
AGCGCTGTATGGAAATCGCCTTTTCGATTATGTCGCCCAAACAGATGGACGAATTCATGAACACCAACGAGTGCAACTTC
GCCATCAGCCTGCCCGACACCAGCCGTTTCCGCGTCAACGCCATGGTTCAGCGCGGTGCCACCGCTCTCGTGTTCCGCAC
CATCACCAGCAATATCCCCAAATTTGAAACGCTCAACCTGCCGCCCGTTTTGCAGAAAATCGCCATGCAGAAACGCGGCC
TGGTCATTTTCGTGGGCGGCACCGGTTCGGGCAAATCCACCTCGCTGGCCTCGATGATCGACTACCGCAACGAAAACAGC
CACGACCACATCATCACCATCGAAGACCCCATCGAATTCGTGCACCAGCACAAAAACTGCATCATCACCCAGCGCGAAGT
GGGCGTGGACACCGAAAACTGGTTCGCCGCCCTGAAAAACACCCTCCGCCAAGCTCCCGACGTGATACTGATCGGCGAAA
TCCGCGACCGCGAAACGATGGACTACGCCATCGCCTTTGCCGAAACCGGCCACCTGTGCATGGCCACCCTGCACGCCAAC
AGCACCAACCAGGCACTCGACCGCATCATCAACTTCTTCCCCGAAGAACGCCGCGTGCAGCTGCTTACCGACCTGTCGCT
CAACCTGCAAGCCTTCATCTCGCAGCGTCTGGTTCCGCGCGAGGGCGGCAAAGGCCGCGTGGCCGCTGTGGAAGTGCTGC
TCAACTCGCCGCTGATTGCCGAACTGATCCACAACGGCGACGTGCACTCGATTAAAGAAATCATGAAAAAATCGCAGAGC
ATGGGCATGCAAACCTTCGACCAAGCCTTATACGAGCTGTATGAAAACGGCCATATCAGCTTTCAAGACGCCATCAAAAA
CGCCGATTCCGCCCACGACCTGCGCCTCGACATCCAACTGCGCAGCCGCCGCGCACAAAACGCCGGGCCGGATCTGGAGC
TGATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1X3DEU8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.795

93.867

0.571
  pilU Acinetobacter baylyi ADP1

57.349

92.533

0.531
  pilU Vibrio cholerae strain A1552

53.482

95.733

0.512
  pilT Legionella pneumophila strain Lp02

43.713

89.067

0.389
  pilT Legionella pneumophila strain ERS1305867

43.713

89.067

0.389
  pilT Acinetobacter baumannii strain A118

43.413

89.067

0.387
  pilT Acinetobacter nosocomialis M2

43.413

89.067

0.387
  pilT Acinetobacter baumannii D1279779

43.413

89.067

0.387
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.604

90.133

0.384
  pilT Acinetobacter baylyi ADP1

42.353

90.667

0.384
  pilT Pseudomonas aeruginosa PAK

42.012

90.133

0.379
  pilT Pseudomonas stutzeri DSM 10701

42.012

90.133

0.379
  pilT Neisseria meningitidis 8013

40.87

92

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.042

88.8

0.373
  pilT Vibrio cholerae strain A1552

42.042

88.8

0.373
  pilT Neisseria gonorrhoeae MS11

40.407

91.733

0.371