Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H3L95_RS10100 Genome accession   NZ_CP059566
Coordinates   2112045..2113091 (+) Length   348 a.a.
NCBI ID   WP_003759783.1    Uniprot ID   A0A0C1GQQ0
Organism   Neisseria sicca strain DSM 17713     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2107045..2118091
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3L95_RS10080 (H3L95_10080) dksA 2108927..2109343 (-) 417 WP_003744416.1 RNA polymerase-binding protein DksA -
  H3L95_RS10085 (H3L95_10085) - 2109537..2110091 (-) 555 WP_003759775.1 YggT family protein -
  H3L95_RS10090 (H3L95_10090) proC 2110094..2110891 (-) 798 WP_003759777.1 pyrroline-5-carboxylate reductase -
  H3L95_RS10095 (H3L95_10095) - 2111158..2111865 (-) 708 WP_003759781.1 YggS family pyridoxal phosphate-dependent enzyme -
  H3L95_RS10100 (H3L95_10100) pilT 2112045..2113091 (+) 1047 WP_003759783.1 type IV pilus twitching motility protein PilT Machinery gene
  H3L95_RS10105 (H3L95_10105) - 2113261..2114493 (+) 1233 WP_003759784.1 PilT/PilU family type 4a pilus ATPase -
  H3L95_RS10110 (H3L95_10110) - 2114863..2115699 (-) 837 WP_003759786.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  H3L95_RS10115 (H3L95_10115) - 2115701..2115817 (-) 117 Protein_1973 IS5/IS1182 family transposase -
  H3L95_RS10120 (H3L95_10120) - 2115876..2116058 (-) 183 WP_003759788.1 hypothetical protein -
  H3L95_RS10125 (H3L95_10125) - 2116282..2116818 (+) 537 WP_003759792.1 porin family protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38114.58 Da        Isoelectric Point: 7.3729

>NTDB_id=470741 H3L95_RS10100 WP_003759783.1 2112045..2113091(+) (pilT) [Neisseria sicca strain DSM 17713]
MQITDLLAFGVKNKASDLHLSAGMSPMIRVHGDIRRINLPEMSAEEVGNMVTSVMNDHQRKLFQQDLEVDFSFELPNVAR
FRVNAFTSNRGPAAVFRTIPSAVLTLEDLRAPRIFQKIADNPRGLVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
PIEFVHQSKKALVNQRELHQHTHSFANALRSALREDPDVILVGEMRDPETIGLALTAAETGHLVFGTLHTTGAAKTVDRI
VDVFPAGEKEMVRSMLSESLRAVISQTLLKTRDGNGRVAAHEILISTPAVRNLIRENKIAQINSALQTGQSHGMQTLDQA
LQTLVRQGTISPDVARSKAQNSDSMTIV

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=470741 H3L95_RS10100 WP_003759783.1 2112045..2113091(+) (pilT) [Neisseria sicca strain DSM 17713]
ATGCAGATTACCGACTTACTCGCTTTCGGCGTCAAAAACAAAGCTTCCGACCTTCACTTAAGCGCCGGAATGTCCCCCAT
GATCCGCGTTCACGGCGATATCCGCCGCATCAACCTGCCCGAAATGTCCGCCGAAGAAGTCGGCAACATGGTTACTTCGG
TCATGAACGACCACCAGCGCAAACTGTTCCAACAAGATTTGGAAGTGGACTTCTCCTTCGAGCTGCCCAACGTCGCCCGC
TTCCGTGTCAACGCATTTACCTCCAACCGCGGCCCTGCCGCCGTCTTCCGTACCATTCCGAGCGCCGTATTGACGCTGGA
AGACCTGCGCGCCCCGCGTATTTTCCAAAAAATCGCCGACAACCCGCGCGGCTTGGTATTGGTAACCGGCCCGACCGGTT
CCGGTAAATCCACCACGCTGGCGGCGATGATCAACTACATCAACGAAACCCAACCCGCACACATACTCACCATCGAAGAC
CCGATCGAGTTCGTCCACCAAAGTAAAAAAGCCCTCGTCAACCAGCGCGAGCTGCACCAACACACCCACAGCTTCGCCAA
CGCCCTGCGTTCCGCATTGCGTGAAGACCCCGACGTAATCCTCGTAGGCGAGATGCGCGACCCCGAAACCATCGGCCTCG
CGCTGACTGCCGCCGAAACCGGCCACTTGGTATTCGGTACGCTGCACACCACCGGCGCCGCCAAAACCGTGGACCGTATC
GTTGACGTATTCCCGGCCGGCGAAAAAGAAATGGTGCGTTCGATGTTGTCCGAATCCCTGCGCGCAGTTATTTCGCAAAC
CCTGCTGAAAACCCGCGACGGCAACGGCCGTGTCGCCGCACACGAAATCCTCATTTCCACCCCCGCAGTGCGCAACCTGA
TCCGCGAAAACAAAATCGCCCAAATCAACTCCGCGCTGCAAACCGGTCAATCACACGGCATGCAAACCCTCGACCAAGCG
CTGCAAACCCTCGTCCGCCAAGGCACCATCAGCCCCGACGTAGCCCGTAGCAAAGCGCAAAACAGCGACAGCATGACCAT
CGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C1GQQ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

87.896

99.713

0.876
  pilT Neisseria gonorrhoeae MS11

87.32

99.713

0.871
  pilT Acinetobacter nosocomialis M2

71.014

99.138

0.704
  pilT Acinetobacter baumannii D1279779

71.014

99.138

0.704
  pilT Acinetobacter baumannii strain A118

71.014

99.138

0.704
  pilT Pseudomonas stutzeri DSM 10701

70.145

99.138

0.695
  pilT Pseudomonas aeruginosa PAK

70.294

97.701

0.687
  pilT Acinetobacter baylyi ADP1

68.406

99.138

0.678
  pilT Legionella pneumophila strain Lp02

66.569

97.989

0.652
  pilT Legionella pneumophila strain ERS1305867

66.569

97.989

0.652
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.706

97.701

0.632
  pilT Vibrio cholerae strain A1552

64.706

97.701

0.632
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.622

97.414

0.503
  pilU Pseudomonas stutzeri DSM 10701

41.954

100

0.42
  pilU Vibrio cholerae strain A1552

39.368

100

0.394
  pilU Acinetobacter baylyi ADP1

40.361

95.402

0.385