Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H3L98_RS09400 Genome accession   NZ_CP059563
Coordinates   1835371..1836411 (-) Length   346 a.a.
NCBI ID   WP_027021800.1    Uniprot ID   -
Organism   Conchiformibius steedae strain DSM 2580     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1830371..1841411
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3L98_RS09375 (H3L98_09385) - 1830484..1831365 (-) 882 WP_027021795.1 prephenate dehydrogenase -
  H3L98_RS09380 (H3L98_09390) waaF 1831365..1832366 (-) 1002 WP_034333137.1 lipopolysaccharide heptosyltransferase II -
  H3L98_RS09385 (H3L98_09395) - 1832363..1833502 (-) 1140 WP_027021797.1 5-(carboxyamino)imidazole ribonucleotide synthase -
  H3L98_RS09390 (H3L98_09400) - 1833499..1833939 (-) 441 WP_027021798.1 peroxiredoxin -
  H3L98_RS09395 (H3L98_09405) - 1834031..1835200 (-) 1170 WP_420838896.1 PilT/PilU family type 4a pilus ATPase -
  H3L98_RS09400 (H3L98_09410) pilT 1835371..1836411 (-) 1041 WP_027021800.1 type IV pilus twitching motility protein PilT Machinery gene
  H3L98_RS09405 (H3L98_09415) - 1836523..1837221 (-) 699 WP_027021801.1 pseudouridine synthase -
  H3L98_RS09410 (H3L98_09420) - 1837225..1837587 (-) 363 WP_156932247.1 hypothetical protein -
  H3L98_RS09415 (H3L98_09425) - 1837739..1839157 (-) 1419 WP_027021803.1 sodium:proton antiporter -
  H3L98_RS09420 (H3L98_09430) - 1839398..1839742 (-) 345 WP_027021804.1 hypothetical protein -
  H3L98_RS09425 (H3L98_09435) - 1840087..1841019 (-) 933 WP_027021805.1 branched-chain amino acid transaminase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 37900.45 Da        Isoelectric Point: 7.6533

>NTDB_id=470662 H3L98_RS09400 WP_027021800.1 1835371..1836411(-) (pilT) [Conchiformibius steedae strain DSM 2580]
MQITDLLAFGVKNKASDLHLSAGLPPMIRVHGDIRRINLPEMSSEDVGNMISSVMNDQQRKSYQQNLETDFSFELPSVAR
FRVNAFNTNRGPAAVFRTIPSKVLTLEELKAPRVFQKIADTPRGLVLVTGPTGSGKSTTLAAMIDYVNQNHPGHILTIED
PIEFVHQSKKSLVNQRELHEHTHSFANALKSALREDPDIILVGEMRDPETIGLALTAAETGHLVFGTLHTTSAAKTVDRI
IDVFPAGEKEMVRSMLSESLRAVIAQTLMKTKDGNGRVAAHEILISTAAVRNLIRENKIAQLGSVLQTGQNFGMQTLDQC
LQDLVRRGVVSLEAARAKAANPDMIS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=470662 H3L98_RS09400 WP_027021800.1 1835371..1836411(-) (pilT) [Conchiformibius steedae strain DSM 2580]
ATGCAAATTACCGACCTGCTTGCATTCGGCGTGAAAAACAAAGCGTCCGACTTACACCTTTCCGCCGGGCTGCCGCCCAT
GATTCGCGTTCACGGCGATATCCGCCGCATCAACCTGCCAGAGATGTCTTCCGAAGATGTCGGCAATATGATTAGCTCGG
TGATGAACGACCAGCAGCGCAAAAGCTATCAGCAAAATCTGGAAACCGACTTCTCGTTTGAGCTGCCCAGCGTGGCGCGT
TTCCGTGTGAATGCGTTTAACACCAACCGCGGACCTGCTGCCGTGTTCCGTACCATTCCCAGCAAAGTGCTGACTTTGGA
AGAACTGAAAGCACCGCGCGTGTTCCAAAAAATTGCCGATACCCCGCGCGGGTTGGTGTTGGTTACTGGACCCACGGGTT
CGGGTAAATCCACCACCTTGGCGGCGATGATTGACTATGTAAACCAAAACCATCCCGGTCATATCTTAACCATTGAAGAC
CCGATTGAATTTGTGCATCAAAGTAAAAAATCACTTGTCAATCAGCGCGAATTGCACGAACATACGCACAGTTTTGCCAA
TGCCTTGAAATCCGCCCTGCGTGAAGACCCCGATATTATTTTGGTGGGCGAGATGCGCGACCCCGAAACCATCGGTTTGG
CACTGACTGCCGCCGAAACGGGTCACTTGGTGTTCGGCACCCTGCACACCACTTCTGCCGCCAAAACCGTTGACCGTATT
ATTGACGTGTTCCCCGCAGGCGAAAAGGAAATGGTGCGTTCCATGCTTTCCGAATCACTGCGTGCGGTGATTGCACAAAC
GCTGATGAAAACCAAAGACGGCAACGGACGTGTGGCGGCGCATGAAATTCTGATTTCTACCGCAGCGGTGCGTAACCTGA
TTCGCGAAAACAAAATTGCCCAGCTTGGCTCGGTACTGCAAACCGGTCAAAACTTCGGTATGCAAACCCTTGACCAATGT
TTGCAGGATTTGGTACGCCGTGGCGTGGTGTCTTTGGAAGCAGCTCGTGCCAAAGCCGCCAACCCCGATATGATTTCCTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

81.792

100

0.818
  pilT Neisseria meningitidis 8013

81.214

100

0.812
  pilT Acinetobacter baumannii D1279779

71.429

99.133

0.708
  pilT Acinetobacter baumannii strain A118

71.429

99.133

0.708
  pilT Acinetobacter nosocomialis M2

71.429

99.133

0.708
  pilT Pseudomonas aeruginosa PAK

71.429

99.133

0.708
  pilT Pseudomonas stutzeri DSM 10701

71.137

99.133

0.705
  pilT Acinetobacter baylyi ADP1

69.679

99.133

0.691
  pilT Legionella pneumophila strain Lp02

66.667

99.711

0.665
  pilT Legionella pneumophila strain ERS1305867

66.667

99.711

0.665
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.385

97.688

0.639
  pilT Vibrio cholerae strain A1552

65.385

97.688

0.639
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.585

98.844

0.5
  pilU Pseudomonas stutzeri DSM 10701

44.478

96.821

0.431
  pilU Vibrio cholerae strain A1552

43.385

93.931

0.408
  pilU Acinetobacter baylyi ADP1

39.244

99.422

0.39