Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H2677_RS04245 Genome accession   NZ_CP059558
Coordinates   885016..886053 (-) Length   345 a.a.
NCBI ID   WP_004960020.1    Uniprot ID   -
Organism   Acinetobacter junii strain YR7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 880016..891053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2677_RS04215 (H2677_04215) - 880676..881131 (+) 456 WP_004913914.1 bacteriohemerythrin -
  H2677_RS04220 (H2677_04220) - 881370..882449 (+) 1080 WP_212639201.1 hypothetical protein -
  H2677_RS04225 (H2677_04225) - 882449..882772 (+) 324 WP_126131884.1 RnfH family protein -
  H2677_RS04230 (H2677_04230) - 882828..883226 (-) 399 WP_004913908.1 outer membrane protein assembly factor BamE -
  H2677_RS04235 (H2677_04235) fur 883338..883775 (+) 438 WP_004952725.1 ferric iron uptake transcriptional regulator -
  H2677_RS04240 (H2677_04240) pilU 883872..884990 (-) 1119 WP_004913902.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H2677_RS04245 (H2677_04245) pilT 885016..886053 (-) 1038 WP_004960020.1 type IV pilus twitching motility protein PilT Machinery gene
  H2677_RS04250 (H2677_04250) - 886181..886867 (+) 687 WP_212639202.1 YggS family pyridoxal phosphate-dependent enzyme -
  H2677_RS04255 (H2677_04255) - 886864..890460 (-) 3597 WP_212639203.1 AAA family ATPase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38437.01 Da        Isoelectric Point: 6.4330

>NTDB_id=470589 H2677_RS04245 WP_004960020.1 885016..886053(-) (pilT) [Acinetobacter junii strain YR7]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEDLETDFSFEVPNIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGTIFKDICDYPRGIVLVTGPTGSGKSTTLAAMIDYINEHRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLVRENKVAQMYSAIQTGANYGMTTLDQSL
KNLVSRGIISPQTARTVAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=470589 H2677_RS04245 WP_004960020.1 885016..886053(-) (pilT) [Acinetobacter junii strain YR7]
ATGGATATTACAGAATTACTCGCGTTTTCAGTAAAAAACGGTGCTTCGGACTTACACCTTTCAGCGGGTATGCCACCAAT
GATTCGCGTGGATGGTGAAGTTCGCCGTATCAATTTACCAGCATTAGAACATAAAGATGTACACCGTTTAGTGTACGACA
TTATGAATGACAAACAACGTCGAGATTACGAAGAAGATCTAGAAACGGACTTTTCATTTGAAGTACCAAACATTGCCCGT
TTCCGTGTAAACGCATTTAACCAAAACCGTGGTGCGGGTGCTGTATTCCGTACCATTCCATCTAAAGTCTTAACCATGGA
AGACTTGGGTTTAGGAACAATTTTCAAAGATATTTGTGACTACCCGCGTGGTATTGTTCTGGTCACTGGTCCAACGGGTT
CAGGTAAATCAACGACATTGGCTGCAATGATTGATTATATTAATGAGCATCGTTATGACCATATTTTAACGGTTGAAGAC
CCAATCGAATTTGTACATCAATCAAAGAAATGTTTGATTAACCAGCGTGAAGTGCATCGTGATACACATGGCTTTAACGA
AGCACTTCGTTCAGCCCTTCGTGAAGACCCTGATATTATCCTAGTCGGTGAGATGCGTGACCTAGAGACAATTCGTCTTG
CTCTAACCGCAGCAGAAACAGGTCACTTAGTATTTGGTACTCTGCATACCACCTCAGCAGCAAAAACCATTGACCGTGTG
ATCGACGTATTCCCTGCCGAAGAAAAAGATATGGTTCGCGCGATGTTGTCCGAATCATTACAAGCAGTAATTTCACAAAC
ATTGTTGAAAAAGAATGGCGGTGGTCGTGTTGCAGCGCATGAGATCATGATCGGTATTCCTGCGATTCGTAACCTTGTTC
GTGAAAACAAAGTTGCACAGATGTACTCTGCGATTCAGACAGGTGCTAACTACGGCATGACCACACTCGATCAAAGTCTT
AAAAACTTAGTTTCGAGAGGCATCATTAGCCCTCAAACAGCTCGTACTGTTGCTAAACAACCTGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

97.101

100

0.971
  pilT Acinetobacter baumannii D1279779

96.812

100

0.968
  pilT Acinetobacter baumannii strain A118

96.812

100

0.968
  pilT Acinetobacter baylyi ADP1

88.372

99.71

0.881
  pilT Pseudomonas aeruginosa PAK

82.849

99.71

0.826
  pilT Pseudomonas stutzeri DSM 10701

82.558

99.71

0.823
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.238

97.391

0.684
  pilT Vibrio cholerae strain A1552

70.238

97.391

0.684
  pilT Neisseria meningitidis 8013

67.826

100

0.678
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.675
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.513
  pilU Vibrio cholerae strain A1552

41.791

97.101

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.101

0.397
  pilU Acinetobacter baylyi ADP1

39.017

100

0.391