Detailed information NTG0469918

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: JSQ43_RS04385
• Genome accession: NZ_CP069709
• Coordinates: 822176..823156 (+)
• Length: 326 a.a.
• NCBI ID: WP_071999886.1
• Uniprot ID: -
• Organism: Escherichia coli strain EA7
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 817176..828156

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JSQ43_RS04355 (JSQ43_04355)	yggM	817691..818698 (+)	1008	WP_000745244.1	DUF1202 family protein	-
JSQ43_RS04360 (JSQ43_04360)	hemW	818853..819989 (-)	1137	WP_032256677.1	radical SAM family heme chaperone HemW	-
JSQ43_RS04365 (JSQ43_04365)	rdgB	819982..820575 (-)	594	WP_001174735.1	XTP/dITP diphosphatase	-
JSQ43_RS04370 (JSQ43_04370)	yggU	820583..820873 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
JSQ43_RS04375 (JSQ43_04375)	yggT	820870..821436 (-)	567	WP_032237483.1	osmotic shock tolerance protein YggT	-
JSQ43_RS04380 (JSQ43_04380)	yggS	821454..822158 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
JSQ43_RS04385 (JSQ43_04385)	pilT	822176..823156 (+)	981	WP_071999886.1	type IV pilus twitching motility protein PilT	Machinery gene
JSQ43_RS04390 (JSQ43_04390)	ruvX	823331..823747 (-)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
JSQ43_RS04395 (JSQ43_04395)	yqgE	823747..824310 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
JSQ43_RS04400 (JSQ43_04400)	gshB	824419..825369 (-)	951	WP_000593273.1	glutathione synthase	-
JSQ43_RS04405 (JSQ43_04405)	rsmE	825382..826113 (-)	732	WP_001300912.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
JSQ43_RS04410 (JSQ43_04410)	endA	826193..826900 (-)	708	WP_000286500.1	deoxyribonuclease I	-
JSQ43_RS04415 (JSQ43_04415)	yggI	826995..827492 (-)	498	WP_072663006.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35976.14 Da
• Isoelectric Point: 5.7980

>NTDB_id=469918 JSQ43_RS04385 WP_071999886.1 822176..823156(+) (pilT) [Escherichia coli strain EA7]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=469918 JSQ43_RS04385 WP_071999886.1 822176..823156(+) (pilT) [Escherichia coli strain EA7]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATACGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGGAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGA
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.476	100	0.488
pilT	Neisseria gonorrhoeae MS11	48.171	100	0.485
pilT	Acinetobacter baylyi ADP1	46.789	100	0.469
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	46.177	100	0.463
pilT	Legionella pneumophila strain ERS1305867	44.954	100	0.451
pilT	Legionella pneumophila strain Lp02	44.954	100	0.451
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.824	97.546	0.408
pilU	Vibrio cholerae strain A1552	39.514	100	0.399
pilU	Pseudomonas stutzeri DSM 10701	38.554	100	0.393
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362