Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FOLKNPGA_RS05890 Genome accession   NZ_CP059400
Coordinates   1351229..1352263 (+) Length   344 a.a.
NCBI ID   WP_182352120.1    Uniprot ID   -
Organism   Legionella sp. PC1000     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1346229..1357263
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOLKNPGA_RS05860 (FOLKNPGA_01201) - 1346518..1346802 (-) 285 WP_182352114.1 hypothetical protein -
  FOLKNPGA_RS05865 (FOLKNPGA_01202) - 1346891..1348357 (-) 1467 WP_182352115.1 hypothetical protein -
  FOLKNPGA_RS05870 (FOLKNPGA_01204) - 1348671..1348952 (-) 282 WP_182352116.1 hypothetical protein -
  FOLKNPGA_RS05875 (FOLKNPGA_01205) - 1349139..1349705 (-) 567 WP_182352117.1 DUF2845 domain-containing protein -
  FOLKNPGA_RS05880 (FOLKNPGA_01206) - 1349752..1350327 (-) 576 WP_182352118.1 YggT family protein -
  FOLKNPGA_RS05885 (FOLKNPGA_01207) - 1350385..1351071 (-) 687 WP_182352119.1 YggS family pyridoxal phosphate-dependent enzyme -
  FOLKNPGA_RS05890 (FOLKNPGA_01208) pilT 1351229..1352263 (+) 1035 WP_182352120.1 type IV pilus twitching motility protein PilT Machinery gene
  FOLKNPGA_RS05895 (FOLKNPGA_01209) rph 1352457..1353164 (-) 708 WP_182352121.1 ribonuclease PH -
  FOLKNPGA_RS05900 (FOLKNPGA_01210) - 1353704..1354570 (+) 867 WP_182352122.1 YicC/YloC family endoribonuclease -
  FOLKNPGA_RS05905 (FOLKNPGA_01211) gmk 1354576..1355205 (+) 630 WP_182352775.1 guanylate kinase -
  FOLKNPGA_RS05910 (FOLKNPGA_01212) rpoZ 1355237..1355440 (+) 204 WP_010653170.1 DNA-directed RNA polymerase subunit omega -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38337.26 Da        Isoelectric Point: 6.5931

>NTDB_id=469375 FOLKNPGA_RS05890 WP_182352120.1 1351229..1352263(+) (pilT) [Legionella sp. PC1000]
MDIAELLAFSVKNKSSDLHLSAGLPPMIRVDGDLRKINLPALEHKEVIKIIYDVMNDRQRKEFEEFLETDFSFEITNLAR
FRVNVFNQLRGAAAVFRTIPSEILSMNDLGLPAIFEEMASYPKGLVLITGPTGSGKSTTLAAIIDYINSNRYDHILTVED
PIEFVHQSKKCLLNQREVHRDTLSFNAALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTNSATKTINRI
IDVFPAEEKSMVRSMLSESLQAVVAQSLLKKNGGGRVAALEIMICTGAIRNLIREDKVAQMYSSIQTGQAKGMQTLDQHL
TELVKKNIISTHTARDMAIDKALF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=469375 FOLKNPGA_RS05890 WP_182352120.1 1351229..1352263(+) (pilT) [Legionella sp. PC1000]
ATGGATATAGCAGAACTATTGGCGTTTTCAGTAAAAAATAAATCATCAGATTTGCATCTTTCTGCAGGATTACCTCCAAT
GATTCGTGTGGATGGCGATTTGCGCAAAATCAATTTACCAGCCTTGGAACATAAAGAGGTAATAAAAATCATCTATGATG
TGATGAATGACCGGCAAAGGAAAGAATTTGAAGAATTTTTGGAAACTGATTTTTCTTTTGAAATAACTAATCTAGCCCGT
TTTAGGGTGAACGTATTTAATCAACTGCGTGGGGCAGCAGCTGTTTTTCGTACTATTCCTTCTGAAATTTTAAGTATGAA
TGATTTAGGATTACCGGCTATTTTTGAAGAAATGGCCAGTTATCCTAAAGGATTGGTACTTATTACTGGTCCAACAGGAT
CAGGAAAAAGCACGACTCTGGCTGCCATCATCGATTACATCAACTCAAATCGTTATGATCACATTTTAACTGTGGAGGAT
CCTATAGAGTTTGTACATCAAAGCAAAAAATGTTTGTTAAATCAACGCGAGGTTCATCGAGATACACTGAGCTTTAATGC
TGCTTTACGTTCTGCATTACGTGAGGATCCTGATGTGATTTTGGTGGGCGAGTTACGCGATCTGGAAACGATTCGTTTAG
CAATGACCGCCGCGGAAACCGGGCATTTAGTTTTTGGCACCTTACATACTAATTCTGCAACCAAAACCATCAATAGGATC
ATTGATGTGTTCCCCGCTGAGGAAAAATCCATGGTGCGTTCGATGTTATCTGAATCACTGCAGGCTGTAGTCGCGCAATC
CTTATTGAAGAAAAATGGTGGTGGGCGAGTTGCTGCTTTGGAAATTATGATTTGTACCGGTGCGATACGAAATTTAATTC
GTGAAGATAAAGTGGCACAAATGTATTCTTCTATCCAAACAGGTCAGGCAAAAGGCATGCAAACCTTAGATCAGCATTTA
ACTGAACTGGTGAAAAAAAATATAATTTCTACTCATACCGCACGTGATATGGCAATTGATAAGGCTTTATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

90.116

100

0.901
  pilT Legionella pneumophila strain ERS1305867

90.116

100

0.901
  pilT Acinetobacter nosocomialis M2

76.923

98.256

0.756
  pilT Acinetobacter baumannii D1279779

76.923

98.256

0.756
  pilT Acinetobacter baumannii strain A118

76.923

98.256

0.756
  pilT Acinetobacter baylyi ADP1

76.331

98.256

0.75
  pilT Pseudomonas stutzeri DSM 10701

74.852

98.256

0.735
  pilT Pseudomonas aeruginosa PAK

74.556

98.256

0.733
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.389

95.64

0.654
  pilT Vibrio cholerae strain A1552

68.389

95.64

0.654
  pilT Neisseria meningitidis 8013

65.487

98.547

0.645
  pilT Neisseria gonorrhoeae MS11

65.192

98.547

0.642
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.497
  pilU Vibrio cholerae strain A1552

41.791

97.384

0.407
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

39.157

96.512

0.378