Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HZF06_RS09915 Genome accession   NZ_CP059378
Coordinates   2032290..2033345 (+) Length   351 a.a.
NCBI ID   WP_181603384.1    Uniprot ID   -
Organism   Clostridium intestinale strain Lx1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2027290..2038345
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HZF06_RS09895 (HZF06_09895) - 2027857..2029080 (+) 1224 WP_181603382.1 U32 family peptidase -
  HZF06_RS09900 (HZF06_09900) udk 2029092..2029712 (+) 621 WP_181603383.1 uridine kinase -
  HZF06_RS09905 (HZF06_09905) - 2029764..2031368 (+) 1605 WP_243186749.1 penicillin-binding transpeptidase domain-containing protein -
  HZF06_RS09910 (HZF06_09910) sigK 2031440..2032144 (+) 705 WP_021802200.1 RNA polymerase sporulation sigma factor SigK -
  HZF06_RS09915 (HZF06_09915) pilT 2032290..2033345 (+) 1056 WP_181603384.1 type IV pilus twitching motility protein PilT Machinery gene
  HZF06_RS09920 (HZF06_09920) - 2033521..2034795 (+) 1275 WP_181603385.1 glycosyltransferase family 2 protein -
  HZF06_RS09925 (HZF06_09925) ftsZ 2035372..2036469 (+) 1098 WP_181603386.1 cell division protein FtsZ -
  HZF06_RS09930 (HZF06_09930) - 2036640..2037443 (+) 804 WP_181603387.1 sigma-E processing peptidase SpoIIGA -
  HZF06_RS09935 (HZF06_09935) sigE 2037459..2038166 (+) 708 WP_021802195.1 RNA polymerase sporulation sigma factor SigE -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38849.97 Da        Isoelectric Point: 7.0282

>NTDB_id=469156 HZF06_RS09915 WP_181603384.1 2032290..2033345(+) (pilT) [Clostridium intestinale strain Lx1]
MYVLVELLSKTLELGASDLHLTVGIPPVIRVNGGLIKIGSSNLTPVDTEKFAKEILEEKFTEYSQVGEYDTSYSIAGLCR
FRVNVFKQRNSDALALRVIALKIPTLQELSHPPVLKELTEKKRGLVLVTGPTGSGKSTTLAAMINEVNHNREAHVITLED
PIEFLHKHDKCIINQREIGKDSNSYQNALRAILREDPDVILVGEMRDLETIAIAITAAETGHLVFSTLHTIGAAKTIDRI
VDVFPPHQQQQIRIQLSAVLQGIVSQQLVPTADKQSRVAALEVMITTPGIQNIIREGKTHQIESSIQTGSKYGMKTMDMA
LAELYRKGVITQETALSYAVDRETIKRIMLL

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=469156 HZF06_RS09915 WP_181603384.1 2032290..2033345(+) (pilT) [Clostridium intestinale strain Lx1]
ATGTACGTTTTAGTTGAACTGTTAAGTAAAACATTAGAGTTAGGGGCATCAGATTTGCATCTTACAGTAGGAATACCACC
TGTAATTAGAGTTAATGGTGGATTGATTAAAATAGGAAGTTCTAATCTAACTCCTGTAGACACCGAAAAATTTGCAAAAG
AAATACTGGAAGAAAAATTCACAGAATATAGTCAAGTAGGTGAATACGATACATCTTATTCAATTGCTGGATTATGTAGA
TTTAGAGTGAATGTATTTAAACAAAGAAATTCAGACGCATTAGCACTTAGAGTTATTGCATTAAAGATACCCACACTGCA
AGAGCTCAGCCATCCACCTGTATTAAAAGAGTTAACAGAGAAAAAGAGAGGATTAGTTCTAGTTACTGGGCCAACTGGAA
GCGGTAAATCTACTACTTTAGCTGCGATGATTAATGAGGTAAATCATAACAGAGAAGCTCATGTAATTACTTTAGAAGAT
CCTATAGAATTTTTACATAAACATGATAAGTGTATAATTAATCAAAGAGAAATAGGAAAAGATTCAAATAGCTATCAAAA
TGCTTTAAGAGCAATTTTAAGAGAAGACCCTGATGTTATTCTAGTTGGAGAAATGAGAGATCTTGAAACCATAGCTATAG
CAATAACAGCTGCTGAAACAGGACATTTAGTCTTTTCAACATTACATACTATTGGAGCTGCAAAAACTATAGATAGAATA
GTAGATGTTTTCCCTCCACACCAGCAACAACAAATAAGAATTCAATTGTCAGCAGTTTTACAAGGAATTGTTTCTCAACA
ATTGGTACCAACAGCTGATAAACAAAGTAGAGTAGCAGCATTAGAAGTTATGATTACTACCCCAGGAATTCAAAACATAA
TAAGAGAAGGAAAAACCCATCAAATTGAATCATCAATACAAACTGGAAGCAAATATGGAATGAAGACTATGGATATGGCT
TTAGCTGAGTTATATAGAAAAGGTGTTATTACCCAAGAGACAGCCTTAAGTTATGCCGTTGATAGAGAGACTATTAAAAG
AATTATGTTGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

50.882

96.866

0.493
  pilT Legionella pneumophila strain ERS1305867

50.882

96.866

0.493
  pilT Acinetobacter baylyi ADP1

49.563

97.721

0.484
  pilT Pseudomonas aeruginosa PAK

50.298

95.726

0.481
  pilT Acinetobacter baumannii D1279779

48.688

97.721

0.476
  pilT Acinetobacter nosocomialis M2

48.688

97.721

0.476
  pilT Acinetobacter baumannii strain A118

48.688

97.721

0.476
  pilT Neisseria meningitidis 8013

48.105

97.721

0.47
  pilT Pseudomonas stutzeri DSM 10701

49.249

94.872

0.467
  pilT Neisseria gonorrhoeae MS11

47.813

97.721

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.11

98.575

0.464
  pilT Vibrio cholerae strain A1552

49.085

93.447

0.459
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.085

93.447

0.459
  pilU Pseudomonas stutzeri DSM 10701

43.931

98.575

0.433
  pilU Vibrio cholerae strain A1552

39.943

99.145

0.396
  pilU Acinetobacter baylyi ADP1

40.525

97.721

0.396