Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H0S58_RS10470 Genome accession   NZ_CP059079
Coordinates   2240400..2241521 (+) Length   373 a.a.
NCBI ID   WP_053579732.1    Uniprot ID   -
Organism   Acinetobacter sp. TTH0-4 strain 1BD1     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2235400..2246521
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0S58_RS10460 (H0S58_10465) - 2238526..2239212 (-) 687 WP_195726485.1 YggS family pyridoxal phosphate-dependent enzyme -
  H0S58_RS10465 (H0S58_10470) pilT 2239337..2240374 (+) 1038 WP_053579733.1 type IV pilus twitching motility protein PilT Machinery gene
  H0S58_RS10470 (H0S58_10475) pilU 2240400..2241521 (+) 1122 WP_053579732.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H0S58_RS10475 (H0S58_10480) fur 2241576..2242013 (-) 438 WP_053579731.1 ferric iron uptake transcriptional regulator -
  H0S58_RS10480 (H0S58_10485) - 2242126..2242524 (+) 399 WP_053579730.1 outer membrane protein assembly factor BamE -
  H0S58_RS10485 (H0S58_10490) - 2242569..2242892 (-) 324 WP_193891958.1 RnfH family protein -
  H0S58_RS10490 (H0S58_10495) - 2242892..2243974 (-) 1083 WP_195726486.1 hypothetical protein -
  H0S58_RS10495 (H0S58_10500) - 2244115..2244564 (-) 450 WP_092719351.1 bacteriohemerythrin -
  H0S58_RS10500 (H0S58_10505) - 2244903..2245751 (-) 849 WP_092719348.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 42015.33 Da        Isoelectric Point: 6.7815

>NTDB_id=467048 H0S58_RS10470 WP_053579732.1 2240400..2241521(+) (pilU) [Acinetobacter sp. TTH0-4 strain 1BD1]
MDFNDLLNLMIQKQASDLFLTADVEPSMKINGQILPIAKTKLTGEIVGQLLNSIMSDKQRKEFAETRECNFAINNRDKTA
RFRVSAFQQRDHPGMVLRRIETVIPTMDELKLPPILKELAMTKRGIIIFVGATGTGKSTSLASIIGYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHNQLFMDLSLNLRAMVAQQLIPTIDGNSRRAAIEILINSPLISDLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALFDLYKAKQITYKDALKHADSPNDLRLQIKLSEEGADRLMHAGTNITFDGQS

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=467048 H0S58_RS10470 WP_053579732.1 2240400..2241521(+) (pilU) [Acinetobacter sp. TTH0-4 strain 1BD1]
ATGGATTTTAACGACTTACTCAATTTGATGATTCAAAAACAAGCATCAGACTTATTTCTCACTGCTGATGTTGAACCTTC
AATGAAAATTAATGGTCAGATTCTTCCGATTGCAAAAACAAAGCTGACAGGAGAAATAGTGGGTCAGCTTTTAAATTCAA
TCATGAGTGACAAGCAACGTAAAGAATTTGCTGAAACTCGTGAATGTAACTTTGCCATTAATAATCGTGATAAAACTGCG
CGTTTTCGTGTCAGTGCTTTTCAACAGCGTGATCATCCTGGCATGGTATTACGTCGTATCGAAACGGTTATTCCAACTAT
GGATGAGCTCAAGTTACCGCCTATTTTAAAAGAACTGGCGATGACTAAACGCGGTATTATCATCTTTGTAGGAGCAACGG
GAACAGGTAAATCAACGTCTTTGGCTTCTATTATTGGTTATCGTAACCATAATTCCAAAGGTCATATCATTACCATTGAA
GATCCGATTGAATTTATTCATGAACATGCGGGCTGTATTATTACCCAGCGTGAAGTCGGTATTGATACAGACTCTTTTGA
AATTGCGTTAAAGAATACCTTACGTCAAGCACCCGATGTAATTTTGATTGGGGAAATTCGTTCACGTGAAACCATGGACT
ATGCCATTGCCTTTGCTGAAACGGGTCACTTAGTATTTGCAACGCTGCATGCCAACAACGCCAACCAAGCTATTGATCGT
ATTATTCATTTCTTTGAAGCTGACCGTCATAACCAACTCTTTATGGACTTGTCGCTTAATTTAAGAGCCATGGTGGCTCA
GCAGTTGATTCCGACCATAGATGGCAATTCACGTCGAGCGGCCATCGAAATTCTGATTAACTCTCCATTAATTTCAGACC
TGATCCGCAAAGGAGATGTGCATGAAATTAAGGATTTGATGAAGCGCTCACGCGAACTGGGTATGCAAACTTTTGACCAA
GCTTTATTTGATTTATATAAGGCCAAGCAAATTACCTACAAAGATGCACTGAAACATGCGGACTCACCAAACGATTTGCG
TTTACAAATCAAACTCTCAGAAGAAGGGGCTGATCGTTTAATGCATGCTGGCACCAATATTACCTTTGATGGTCAATCTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.397

100

0.764
  pilU Pseudomonas stutzeri DSM 10701

70.141

95.174

0.668
  pilU Vibrio cholerae strain A1552

53.243

99.196

0.528
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.353

92.761

0.402
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.886

94.102

0.375
  pilT Vibrio cholerae strain A1552

39.886

94.102

0.375
  pilT Pseudomonas aeruginosa PAK

40.462

92.761

0.375
  pilT Acinetobacter baumannii strain A118

40.525

91.957

0.373
  pilT Acinetobacter baumannii D1279779

40.525

91.957

0.373
  pilT Acinetobacter baylyi ADP1

40.525

91.957

0.373
  pilT Acinetobacter nosocomialis M2

40.525

91.957

0.373
  pilT Legionella pneumophila strain Lp02

41.846

87.131

0.365
  pilT Legionella pneumophila strain ERS1305867

41.846

87.131

0.365
  pilT Neisseria meningitidis 8013

39.306

92.761

0.365
  pilT Neisseria gonorrhoeae MS11

39.306

92.761

0.365
  pilT Pseudomonas stutzeri DSM 10701

39.017

92.761

0.362