Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FY034_RS07040 Genome accession   NZ_CP058409
Coordinates   1537076..1538167 (+) Length   363 a.a.
NCBI ID   WP_012469712.1    Uniprot ID   B3EAD2
Organism   Trichlorobacter lovleyi strain IAE     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1532076..1543167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FY034_RS07025 (FY034_07025) - 1532539..1534413 (+) 1875 WP_265554709.1 DUF2723 domain-containing protein -
  FY034_RS07030 (FY034_07030) aroE 1534403..1535284 (+) 882 WP_265554711.1 shikimate dehydrogenase -
  FY034_RS07035 (FY034_07035) pilB 1535357..1537063 (+) 1707 WP_265554712.1 type IV-A pilus assembly ATPase PilB -
  FY034_RS07040 (FY034_07040) pilT 1537076..1538167 (+) 1092 WP_012469712.1 type IV pilus twitching motility protein PilT Machinery gene
  FY034_RS07045 (FY034_07045) - 1538196..1539410 (+) 1215 WP_265554715.1 type II secretion system F family protein -
  FY034_RS07050 (FY034_07050) - 1539414..1541063 (+) 1650 WP_265554716.1 two-component system sensor histidine kinase NtrB -
  FY034_RS07055 (FY034_07055) - 1541038..1542408 (+) 1371 WP_265554719.1 sigma-54-dependent transcriptional regulator -

Sequence

Protein


Download         Length: 363 a.a.        Molecular weight: 40617.94 Da        Isoelectric Point: 7.4814

>NTDB_id=464133 FY034_RS07040 WP_012469712.1 1537076..1538167(+) (pilT) [Trichlorobacter lovleyi strain IAE]
MANLHELLKTLVEAGGSDLHITTNTQPQIRVDGKLQKLDIPPLNAVETKQLCYSVLTDTQKHKFEEENELDLSFGVKGLS
RFRGNIFVQRGAVAGVFRVIPYKILSFEELGLPPVVKELAEKPRGLILVTGPTGSGKSTTLASIIDFININRKEHIVTIE
DPIEYLHPHKGCLVNQREVGADTKGFKNALKYVLRQDPDVVLVGELRDLETIEAALTLSETGHLCLATLHTNSCAQTINR
IVDVFPPYQQTQIRAQLSFVLEGVMSQALIPKIGGGRVMSLEIMVPNPAIRNLIREDKVHQIYSQMQVGQEKFGMQTMNQ
SLYSLFSRRLITLDDAMGRTSDPDELRQMINNPTMGMRQQPRR

Nucleotide


Download         Length: 1092 bp        

>NTDB_id=464133 FY034_RS07040 WP_012469712.1 1537076..1538167(+) (pilT) [Trichlorobacter lovleyi strain IAE]
ATGGCTAATCTTCACGAACTTCTGAAAACCCTGGTGGAAGCAGGTGGGTCTGACCTTCACATTACCACCAATACCCAGCC
TCAAATCAGGGTGGATGGTAAGTTGCAAAAGCTCGATATACCACCGTTAAATGCGGTGGAGACCAAGCAGCTCTGCTATT
CGGTGCTGACTGACACCCAAAAACACAAATTTGAAGAGGAAAACGAGCTTGATCTTTCTTTCGGGGTCAAGGGGTTATCC
CGTTTCCGGGGAAACATCTTTGTGCAGCGCGGTGCGGTGGCAGGGGTTTTCCGGGTTATTCCCTACAAGATCCTGTCTTT
TGAAGAGCTTGGCCTGCCACCGGTTGTTAAGGAGCTGGCAGAAAAGCCCCGTGGTCTGATTCTGGTGACCGGACCTACCG
GTAGCGGTAAATCAACCACCCTGGCTTCAATCATTGACTTCATTAACATCAACCGGAAAGAACACATTGTCACCATTGAG
GATCCGATTGAATACCTGCATCCCCACAAAGGCTGTCTGGTGAATCAACGTGAGGTCGGAGCCGATACCAAGGGGTTTAA
AAACGCCCTCAAGTACGTTCTGCGTCAGGACCCTGACGTCGTACTTGTTGGCGAATTACGGGATCTTGAGACGATTGAGG
CTGCCCTGACCCTGTCCGAAACCGGTCACCTCTGTCTGGCGACTCTGCATACCAACTCCTGTGCCCAGACCATCAACCGG
ATTGTGGATGTATTCCCCCCCTATCAGCAGACCCAGATTCGTGCCCAACTCTCCTTTGTGCTGGAAGGGGTGATGTCCCA
GGCGCTGATTCCCAAGATCGGCGGTGGCAGGGTTATGTCGCTCGAAATCATGGTGCCGAACCCTGCAATCCGCAACCTTA
TCAGGGAAGACAAGGTCCACCAGATCTACTCCCAGATGCAGGTCGGCCAGGAAAAATTCGGGATGCAGACCATGAACCAG
TCGCTCTATTCGCTTTTTTCCCGGAGACTGATTACCCTTGATGATGCCATGGGGCGCACCTCTGATCCGGACGAATTGAG
GCAGATGATCAACAATCCAACAATGGGGATGCGTCAGCAACCGCGCCGATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B3EAD2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.562

98.072

0.496
  pilT Legionella pneumophila strain Lp02

54.545

90.909

0.496
  pilT Legionella pneumophila strain ERS1305867

54.545

90.909

0.496
  pilT Pseudomonas aeruginosa PAK

51.744

94.766

0.49
  pilT Acinetobacter nosocomialis M2

50.581

94.766

0.479
  pilT Acinetobacter baumannii strain A118

50.291

94.766

0.477
  pilT Pseudomonas stutzeri DSM 10701

50.291

94.766

0.477
  pilT Acinetobacter baumannii D1279779

50.291

94.766

0.477
  pilT Acinetobacter baylyi ADP1

50

94.766

0.474
  pilT Neisseria meningitidis 8013

48.397

94.49

0.457
  pilT Neisseria gonorrhoeae MS11

47.953

94.215

0.452
  pilT Vibrio cholerae strain A1552

48.632

90.634

0.441
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.632

90.634

0.441
  pilU Pseudomonas stutzeri DSM 10701

41.667

95.868

0.399
  pilU Vibrio cholerae strain A1552

41.092

95.868

0.394
  pilU Acinetobacter baylyi ADP1

40

96.419

0.386