Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HW877_RS20625 Genome accession   NZ_CP058353
Coordinates   4299467..4300489 (-) Length   340 a.a.
NCBI ID   WP_048796770.1    Uniprot ID   -
Organism   Serratia marcescens strain ESE2014     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4294467..4305489
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HW877_RS20595 (HW877_20580) - 4295268..4295780 (+) 513 WP_033639394.1 SprT family zinc-dependent metalloprotease -
  HW877_RS20600 (HW877_20585) endA 4295882..4296577 (+) 696 WP_015379043.1 deoxyribonuclease I -
  HW877_RS20605 (HW877_20590) rsmE 4296647..4297378 (+) 732 WP_244757804.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HW877_RS20610 (HW877_20595) gshB 4297389..4298339 (+) 951 WP_016930084.1 glutathione synthase -
  HW877_RS20615 (HW877_20600) - 4298485..4299048 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  HW877_RS20620 (HW877_20605) ruvX 4299048..4299470 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  HW877_RS20625 (HW877_20610) pilT 4299467..4300489 (-) 1023 WP_048796770.1 type IV pilus twitching motility protein PilT Machinery gene
  HW877_RS20630 (HW877_20615) - 4300510..4301217 (+) 708 WP_089185024.1 YggS family pyridoxal phosphate-dependent enzyme -
  HW877_RS20635 (HW877_20620) proC 4301237..4302058 (+) 822 WP_033639391.1 pyrroline-5-carboxylate reductase -
  HW877_RS20640 (HW877_20625) - 4302090..4302644 (+) 555 WP_004937436.1 YggT family protein -
  HW877_RS20645 (HW877_20630) yggU 4302641..4302931 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  HW877_RS20650 (HW877_20635) - 4302950..4303543 (+) 594 WP_033651321.1 XTP/dITP diphosphatase -
  HW877_RS20655 (HW877_20640) hemW 4303536..4304678 (+) 1143 WP_033635947.1 radical SAM family heme chaperone HemW -
  HW877_RS20660 (HW877_20645) - 4304715..4305149 (-) 435 WP_244757805.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36474.75 Da        Isoelectric Point: 7.2889

>NTDB_id=464033 HW877_RS20625 WP_048796770.1 4299467..4300489(-) (pilT) [Serratia marcescens strain ESE2014]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANVFQQSAGISLALRRIAGQSPSLAELAAPAIVPALLRRDDGLILVTGATGSGKSTTLAAMIDEINRHQQRHVLTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGECAGERAGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=464033 HW877_RS20625 WP_048796770.1 4299467..4300489(-) (pilT) [Serratia marcescens strain ESE2014]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGGGCGGGGAG
CGGCTGCGGGCCAACGTTTTTCAACAAAGCGCGGGGATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATTGTCCCGGCGTTGCTGCGGCGCGACGACGGGCTTATCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCACGTTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCATACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGTCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGCGGACGCGTGGCGATCTTCGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGAGAGTGCGCGGGGGAAAGGGCGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Neisseria meningitidis 8013

50

100

0.5
  pilT Acinetobacter baylyi ADP1

51.057

97.353

0.497
  pilT Pseudomonas aeruginosa PAK

48.974

100

0.491
  pilT Acinetobacter nosocomialis M2

50.606

97.059

0.491
  pilT Acinetobacter baumannii strain A118

50.606

97.059

0.491
  pilT Acinetobacter baumannii D1279779

50.606

97.059

0.491
  pilT Neisseria gonorrhoeae MS11

49.408

99.412

0.491
  pilT Pseudomonas stutzeri DSM 10701

48.378

99.706

0.482
  pilT Legionella pneumophila strain Lp02

48.235

100

0.482
  pilT Legionella pneumophila strain ERS1305867

48.235

100

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.737

100

0.45
  pilU Pseudomonas stutzeri DSM 10701

38.348

99.706

0.382
  pilU Vibrio cholerae strain A1552

39.571

95.882

0.379
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379