Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JNN66_RS04340 Genome accession   NZ_CP068823
Coordinates   904009..904989 (+) Length   326 a.a.
NCBI ID   WP_075862143.1    Uniprot ID   -
Organism   Escherichia coli strain RIVM_C029494     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 899009..909989
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JNN66_RS04310 (JNN66_04310) - 899387..900373 (-) 987 WP_001564022.1 TRAP transporter substrate-binding protein -
  JNN66_RS04315 (JNN66_04315) hemW 900686..901822 (-) 1137 WP_075862142.1 radical SAM family heme chaperone HemW -
  JNN66_RS04320 (JNN66_04320) rdgB 901815..902408 (-) 594 WP_001174738.1 XTP/dITP diphosphatase -
  JNN66_RS04325 (JNN66_04325) yggU 902416..902706 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  JNN66_RS04330 (JNN66_04330) yggT 902703..903269 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  JNN66_RS04335 (JNN66_04335) yggS 903287..903991 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  JNN66_RS04340 (JNN66_04340) pilT 904009..904989 (+) 981 WP_075862143.1 type IV pilus twitching motility protein PilT Machinery gene
  JNN66_RS04345 (JNN66_04345) ruvX 905202..905618 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  JNN66_RS04350 (JNN66_04350) yqgE 905618..906181 (-) 564 WP_001339287.1 YqgE/AlgH family protein -
  JNN66_RS04355 (JNN66_04355) gshB 906290..907240 (-) 951 WP_075862144.1 glutathione synthase -
  JNN66_RS04360 (JNN66_04360) rsmE 907253..907984 (-) 732 WP_001300912.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  JNN66_RS04365 (JNN66_04365) endA 908064..908771 (-) 708 WP_000286500.1 deoxyribonuclease I -
  JNN66_RS04370 (JNN66_04370) yggI 908866..909363 (-) 498 WP_023908971.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35929.12 Da        Isoelectric Point: 5.5860

>NTDB_id=463288 JNN66_RS04340 WP_075862143.1 904009..904989(+) (pilT) [Escherichia coli strain RIVM_C029494]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRTILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPIE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLVNTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=463288 JNN66_RS04340 WP_075862143.1 904009..904989(+) (pilT) [Escherichia coli strain RIVM_C029494]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGACAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAAAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTATTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCCGATGTGATATTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTAGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGGAGTTTACGGGCAGTTTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGGTTAACACACCCGCAGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGACGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

45.566

100

0.457
  pilT Legionella pneumophila strain Lp02

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

97.546

0.414
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

37.346

99.387

0.371