Detailed information NTG0462686

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: HWH77_RS03180
• Genome accession: NZ_CP058216
• Coordinates: 663926..664540 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain LF01
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 658926..669540

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HWH77_RS03150 (HWH77_03155)	rpsT	659226..659492 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
HWH77_RS03155 (HWH77_03160)	holA	659509..660550 (-)	1042	Protein_632	DNA polymerase III subunit delta	-
HWH77_RS03160 (HWH77_03165)	-	660590..660744 (-)	155	Protein_633	hypothetical protein	-
HWH77_RS03165 (HWH77_03170)	-	660785..660919 (+)	135	WP_003152870.1	YqzM family protein	-
HWH77_RS03170 (HWH77_03175)	comEC	660938..663289 (-)	2352	WP_069473513.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
HWH77_RS03175 (HWH77_03180)	-	663290..663859 (-)	570	WP_003152868.1	ComE operon protein 2	-
HWH77_RS03180 (HWH77_03185)	comEA	663926..664540 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
HWH77_RS03185 (HWH77_03190)	comER	664599..665420 (+)	822	WP_014305449.1	late competence protein ComER	-
HWH77_RS03190 (HWH77_03195)	-	665489..666226 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
HWH77_RS03195 (HWH77_03200)	rsfS	666223..666579 (-)	357	WP_003152864.1	ribosome silencing factor	-
HWH77_RS03200 (HWH77_03205)	yqeK	666576..667157 (-)	582	WP_014305452.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
HWH77_RS03205 (HWH77_03210)	-	667147..667716 (-)	570	WP_058906195.1	nicotinate-nucleotide adenylyltransferase	-
HWH77_RS03210 (HWH77_03215)	yhbY	667727..668017 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
HWH77_RS03215 (HWH77_03220)	aroE	668011..668853 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=462686 HWH77_RS03180 WP_003152867.1 663926..664540(-) (comEA) [Bacillus velezensis strain LF01]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=462686 HWH77_RS03180 WP_003152867.1 663926..664540(-) (comEA) [Bacillus velezensis strain LF01]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGAAGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363