Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HVX68_RS18050 Genome accession   NZ_CP057620
Coordinates   3800551..3801531 (-) Length   326 a.a.
NCBI ID   WP_181822040.1    Uniprot ID   -
Organism   Citrobacter sp. RHB21-C01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3795551..3806531
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HVX68_RS18020 (HVX68_18025) - 3796300..3796800 (+) 501 WP_181822036.1 SprT family zinc-dependent metalloprotease -
  HVX68_RS18025 (HVX68_18030) endA 3796892..3797599 (+) 708 WP_181822037.1 deoxyribonuclease I -
  HVX68_RS18030 (HVX68_18035) rsmE 3797671..3798402 (+) 732 WP_181822038.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HVX68_RS18035 (HVX68_18040) gshB 3798415..3799362 (+) 948 WP_181822039.1 glutathione synthase -
  HVX68_RS18040 (HVX68_18045) - 3799538..3800101 (+) 564 WP_042290400.1 YqgE/AlgH family protein -
  HVX68_RS18045 (HVX68_18050) ruvX 3800101..3800517 (+) 417 WP_042290401.1 Holliday junction resolvase RuvX -
  HVX68_RS18050 (HVX68_18055) pilT 3800551..3801531 (-) 981 WP_181822040.1 type IV pilus twitching motility protein PilT Machinery gene
  HVX68_RS18055 (HVX68_18060) - 3801549..3802253 (+) 705 WP_060570948.1 YggS family pyridoxal phosphate-dependent enzyme -
  HVX68_RS18060 (HVX68_18065) - 3802272..3802838 (+) 567 WP_181822041.1 YggT family protein -
  HVX68_RS18065 (HVX68_18070) yggU 3802835..3803125 (+) 291 WP_042290406.1 DUF167 family protein YggU -
  HVX68_RS18070 (HVX68_18075) - 3803133..3803726 (+) 594 WP_181822042.1 XTP/dITP diphosphatase -
  HVX68_RS18075 (HVX68_18080) hemW 3803719..3804855 (+) 1137 WP_181822043.1 radical SAM family heme chaperone HemW -
  HVX68_RS18080 (HVX68_18085) - 3805162..3806148 (+) 987 WP_181822044.1 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35878.09 Da        Isoelectric Point: 7.2104

>NTDB_id=461985 HVX68_RS18050 WP_181822040.1 3800551..3801531(-) (pilT) [Citrobacter sp. RHB21-C01]
MNMEEIVALSVKHNVSDLHLSNAWPARWRKRGRLEAAPFPIPDMDSLLAQWLDARQLAVFQQEGQLDFAVTLTGNQRLRA
SAFRQQRGISLALRLLPSRCATLEALTTPAALPELLASENGLILVTGATGSGKSTTLAAMVDWLNRHTDGHILTLEDPIE
YIYASQRCLIQQREIGMHCSSFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDT
FPAQEKEPVRSQLAGSLRAVLSQKLEPDKQEGRVALFELLINTPATGNLIREGKTHQLPHVIQTGQQAGMMTFAQSWQQR
QAQGRV

Nucleotide


Download         Length: 981 bp        

>NTDB_id=461985 HVX68_RS18050 WP_181822040.1 3800551..3801531(-) (pilT) [Citrobacter sp. RHB21-C01]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTAAGCAATGCGTGGCCGGCACG
ATGGCGCAAGCGAGGACGGCTTGAGGCGGCGCCATTCCCTATACCGGATATGGACAGCCTGTTGGCGCAATGGCTTGATG
CGCGACAGCTGGCGGTGTTTCAGCAGGAAGGGCAGCTTGATTTCGCCGTCACGCTTACGGGTAACCAACGCTTGCGGGCC
AGCGCCTTTCGTCAACAGCGGGGGATATCGCTGGCGCTGCGCTTGTTGCCCTCCCGTTGCGCCACCCTGGAAGCGCTGAC
GACGCCTGCGGCACTGCCTGAACTGCTTGCCAGCGAGAACGGACTGATACTGGTGACCGGAGCGACGGGCAGCGGTAAAT
CCACCACCTTGGCGGCAATGGTGGACTGGCTCAACCGCCATACCGACGGACATATTCTGACGCTGGAAGATCCCATTGAG
TACATTTACGCCAGCCAACGCTGCCTGATTCAACAGCGTGAGATCGGTATGCATTGCTCATCGTTTGCCGCGGGATTACG
CGCGGCGCTGCGGGAAGATCCAGACGTTATTCTGCTCGGGGAACTGCGGGACAGCGAGACCATCCGCCTGGCGCTGACGG
CGGCGGAAACCGGGCATCTGGTATTGGCGACGCTACATACCCGCGGCGCGGCGCAGGCCGTCGAGCGGCTGGTGGATACC
TTTCCGGCGCAGGAAAAAGAGCCGGTGCGCAGCCAACTGGCGGGCAGTCTGCGCGCCGTTCTGTCGCAAAAGCTGGAGCC
CGACAAGCAGGAAGGGCGCGTTGCGCTGTTTGAACTGCTGATCAACACCCCAGCGACGGGAAATTTAATTCGTGAGGGCA
AAACGCACCAACTACCCCATGTCATTCAGACCGGACAGCAGGCAGGAATGATGACTTTTGCCCAGAGTTGGCAGCAGCGT
CAGGCGCAGGGGCGAGTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.632

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.632

100

0.491
  pilT Neisseria meningitidis 8013

47.576

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.273

100

0.479
  pilT Pseudomonas stutzeri DSM 10701

46.505

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.201

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

45.593

100

0.46
  pilT Acinetobacter baumannii strain A118

45.593

100

0.46
  pilT Acinetobacter nosocomialis M2

45.289

100

0.457
  pilT Legionella pneumophila strain ERS1305867

46.226

97.546

0.451
  pilT Legionella pneumophila strain Lp02

46.226

97.546

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.271

97.239

0.411
  pilU Vibrio cholerae strain A1552

39.752

98.773

0.393
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Acinetobacter baylyi ADP1

37.538

99.693

0.374